Age-Related Changes in the Regenerative Potential of Adipose-Derived Stem Cells Isolated from the Prominent Fat Pads in Human Lower Eyelids

Ye, Xinhai; Liao, Caihe; Liu, Guangpeng; Xu, Yipin; Tan, Jian; Zhang, Song

doi:10.1371/journal.pone.0166590

Cited by 31 publications

(35 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For chondrogenic or osteogenic regeneration, the spontaneous adipogenic differentiation of ADSCs should be minimized as possible. Adipogenic, chondrogenic, and osteogenic differentiation was observed in passage 3 in many studies (29)(30)(31)(32)(33)(34). The results of the present study indicated that the impact of spontaneous adipogenic differentiation reduced sufficiently after passage 3, and the cells passaged more than three times were probably suitable for chondrogenic and osteogenic regeneration.…”

Section: Discussionsupporting

confidence: 58%

Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

Yang

Zhang

2018

Mol Med Report

View full text Add to dashboard Cite

Primary adipose-derived stem cells (ADSCs) are a mixture of cell types including preadipocytes having the ability to spontaneously differentiate into adipocytes. The aim of the present study was to compare the spontaneous adipogenic differentiation potential of ADSCs at different passages to determine whether it decreased with continuous cell passages. Mouse ADSCs (mADSCs) were harvested and cells from passages 1 to 5 were used for experiments. The proliferation of mADSCs at different passages was tested using the cell counting kit‑8 assay. Reverse transcription‑quantitative polymerase chain reaction was used to determine relative mRNA expression levels of CCAAT/enhancer binding protein α (Cebpa and C/EBPα) and peroxisome proliferator‑activated receptor γ (Pparg and PPARγ), and western blot analysis was used to investigate C/EBPα and PPARγ protein expression. The cells were cultured using DMEM. The fixed cells were then stained using Oil Red O on days 14 and 28, and the obtained extracted dye was monitored for absorbance. The 510 nm absorbance from passages 1 to 5 was observed to be statistically different. The relative expression levels of Cebpa and Pparg for mADSCs from passage 1 were significantly higher when compared with those for mADSCs from passages 2 to 5 on days 3, 5 and 7. However, no difference was identified in the expression levels of proteins C/EBPα and PPARγ for different passages. Although the mRNA expression levels of Pparg from passages 4 to 5 were significantly higher when compared with those from passage 1, the results of Oil Red O absorbance, mRNA expression levels of Cebpa, and the protein expression levels of C/EBPα and PPARγ exhibited no difference between mADSCs from passages 1 to 5 when cultured with induced adipogenic differentiation medium. Therefore, it was concluded that the spontaneous adipogenic differentiation potential of ADSCs decreased with continuous cell passages.

show abstract

Section: Discussionsupporting

confidence: 58%

Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

Yang

Zhang

2018

Mol Med Report

View full text Add to dashboard Cite

show abstract

“…We also found that the synovial membranes obtained from older osteoarthritis patients contained Muse-like cells. In general, although the somatic stem cell number declines with age [17], [18], [19], our results suggest that synovial Muse-like cells may be a potential autologous cell source for cartilage regeneration and regenerative therapy in older patients. Considering that the low yield of Muse-like cells may be caused by cell damage accompanying FACS sorting, an alternative procedure for preparation of SSEA-3-positive cells may improve the yield of Muse-like cells.…”

Section: Discussionmentioning

confidence: 97%

Multilineage-differentiating stress-enduring (Muse)-like cells exist in synovial tissue

Toyoda

Sato

Takahashi

et al. 2019

Regenerative Therapy

View full text Add to dashboard Cite

IntroductionCartilage regeneration is a promising therapy for restoring joint function in patients with cartilage defects. The limited availability of autologous chondrocytes or chondrogenic progenitor cells is an obstacle to its clinical application. We investigated the existence and chondrogenic potential of synovial membrane-derived multilineage-differentiating stress-enduring (Muse)-like cells as an alternative cell source for cartilage regeneration.MethodsCells positive for stage-specific embryonic antigen-3 (SSEA-3), a marker of Muse cells, were isolated from the synovial membranes of 6 of 8 patients (median age, 53.5 years; range 36–72 years) by fluorescence-activated cell sorting. SSEA-3-positive cells were cultured in methylcellulose to examine their ability to form Muse clusters that are similar to the embryoid bodies formed by human embryonic stem cells. Muse clusters were expanded and chondrogenic potential of M-cluster-derived MSCs examined using a pellet culture system. Chondrogenic differentiation was evaluated by proteoglycan, safranin O, toluidine blue and type II collagen staining. To evaluate the practicality of the procedure for isolating Muse-like cells, we compared chondrogenic potential of M-cluster derived MSCs with expanded cells derived from the clusters formed by unsorted synovial cells.ResultsSynovial membranes contained SSEA-3-positive cells that after isolation exhibited Muse-like characteristics such as forming clusters that expressed NANOG, OCT3/4, and SOX2. In the pellet culture system, cell pellets created from the M-cluster-derived MSCs exhibited an increase in wet weight, which implied an increase in extracellular matrix production, displayed metachromasia with toluidine blue and safranin O staining and were aggrecan-positive and type II collagen-positive by immunostaining. Unsorted synovial cells also formed clusters in methylcellulose culture, and the expanded cell population derived from them exhibited chondrogenic potential. The histological and immunohistochemical appearance of chondrogenic pellet created from unsorted synovial cell-derived cells were comparable with that from M-cluster-derived MSCs.ConclusionsMuse-like cells can be isolated from the human synovial membrane, even from older patients, and therefore may provide a source of multipotent cells for regenerative medicine. In addition, the cluster-forming cell population within synovial cells also has excellent chondrogenic potential. These cells may provide a more practical option for cartilage regeneration.

show abstract

“…Ye and collaborators compared ASCs isolated from orbital AT of old-versus-young donors, and found fewer progenitor cells, reduced proliferative rates, increased senescent features and decreased trilineage potential, despite no significant differences in overall cellular yield and immunophenotype [87]. Finally, a significant decrease in ASCs' yield and angiogenic capacity, has been demonstrated also in visceral fat depots of elder individuals [88].…”

Section: Age-related Changes In Ascs' Biology and Regenerative Propermentioning

confidence: 99%

Adipose-derived stem cell therapies for bone regeneration

Barba

Taranto

Lattanzi

2017

Expert Opinion on Biological Therapy

View full text Add to dashboard Cite

Cell-based therapies exploit the heterogeneous and self-sufficient biological environment of stem cells to restore, maintain and improve tissue functions. Adipose-derived stem cells (ASCs) are, to this aim, promising cell types thanks to advantageous isolation procedures, growth kinetics, plasticity and trophic properties. Specifically, bone regeneration represents a suitable, though often challenging, target setting to test and apply ASC-based therapeutic strategies. Areas covered: ASCs are extremely plastic and secrete bioactive peptides that mediate paracrine functions, mediating their trophic actions in vivo. Numerous preclinical studies demonstrated that ASCs improve bone healing. Clinical trials are ongoing to validate the clinical feasibility of these approaches. This review is intended to define the state-of-the-art on ASCs, encompassing the biological features that make them suitable for bone regenerative strategies, and to provide an update on existing preclinical and clinical applications. Expert opinion: ASCs offer numerous advantages over other stem cells in terms of feasibility of clinical translation. Data obtained from in vivo experimentation are encouraging, and clinical trials are ongoing. More robust validations are thus expected to be achieved during the next few years, and will likely pave the way to optimized patient-tailored treatments for bone regeneration.

show abstract

Age-Related Changes in the Regenerative Potential of Adipose-Derived Stem Cells Isolated from the Prominent Fat Pads in Human Lower Eyelids

Cited by 31 publications

References 33 publications

Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

Multilineage-differentiating stress-enduring (Muse)-like cells exist in synovial tissue

Adipose-derived stem cell therapies for bone regeneration

Contact Info

Product

Resources

About