Age-related changes in the optic nerve of Sprague-Dawley rats: An ultrastructural and immunohistochemical study

Yassa, Hanan Dawood

doi:10.1016/j.acthis.2014.05.001

Cited by 8 publications

(4 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The primary antibodies (Table ) used in the present study are well‐characterized markers of ACs and RGCs in mouse retina. Astrocytes and Muller cells were labeled with an antibody against glial fibrillary acidic protein (GFAP) as described in previous studies (Yassa, ; Akopian et al, ). The Brn3a antibody has been shown to selectively label RGCs in the rodent retina (Nadal‐Nicolas et al, ; Galiando‐Romero et al, ).…”

Section: Methodsmentioning

confidence: 99%

Amacrine cells coupled to ganglion cells via gap junctions are highly vulnerable in glaucomatous mouse retinas

Akopian

Kumar

Ramakrishnan

et al. 2016

J of Comparative Neurology

View full text Add to dashboard Cite

We determined whether the structural and functional integrity of amacrine cells (ACs), the largest cohort of neurons in the mammalian retina, are affected in glaucoma. Intraocular injection of microbeads was made in mouse eyes to elevate intraocular pressure as a model of experimental glaucoma. Specific immunocytochemical markers were used to identify AC and displaced (d)ACs subpopulations in both the inner nuclear and ganglion cell layers, respectively, and to distinguish them from retinal ganglion cells (RGCs). Calretinin- and γ-aminobutyric acid (GABA)-immunoreactive (IR) cells were highly vulnerable to glaucomatous damage, whereas choline acetyltransferase (ChAT)-positive and glycinergic AC subtypes were unaffected. The AC loss began 4 weeks after initial microbead injection, corresponding to the time course of RGC loss. Recordings of electroretinogram (ERG) oscillatory potentials and scotopic threshold responses, which reflect AC and RGC activity, were significantly attenuated in glaucomatous eyes following a time course that matched that of the AC and RGC loss. Moreover, we found that it was the ACs coupled to RGCs via gap junctions that were lost in glaucoma, whereas uncoupled ACs were largely unaffected. Our results suggest that AC loss in glaucoma occurs secondary to RGC death through the gap junction-mediated bystander effect. J. Comp. Neurol., 2016. © 2016 Wiley Periodicals, Inc.

show abstract

Section: Methodsmentioning

confidence: 99%

Amacrine cells coupled to ganglion cells via gap junctions are highly vulnerable in glaucomatous mouse retinas

Akopian

Kumar

Ramakrishnan

et al. 2016

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…The optic nerve proteome included astrocytic proteins (glial fibrillary acidic protein, aquaporin‐4) , oligodendrocytic proteins (laminin, proteolipid protein, fibronectin) and myelin proteins (myelin basic protein, myelin‐associated glycoprotein) . Paranodal structural proteins included neurofascin and contactin, septin 2, α, β, and γ adducin, and endophilin.…”

Section: Unambiguous Protein Isoforms Identified In Human Optic Nervementioning

confidence: 99%

The proteome of normal human retrobulbar optic nerve and sclera

et al. 2016

View full text Add to dashboard Cite

The optic nerve is a white matter tract that conveys visual information to the brain. The sclera comprises the white, protective outer layer of the eye. A characterization of the proteome of normal human retrobulbar optic nerve and sclera may facilitate studies of the eye. We conducted a proteomic analysis of optic nerve and sclera from five adults. Proteins were fractionated using SDS-PAGE. After in-gel digestion, peptides were analyzed using LC-MS/MS on an Orbitrap Elite mass spectrometer. We identified 2711 non-redundant proteins in retrobulbar optic nerve and 1945 non-redundant proteins in sclera. Optic nerve proteins included proteins expressed by oligodendrocytes (laminin, proteolipid protein, fibronectin), myelin proteins (myelin basic protein, myelin-associated glycoprotein), and paranodal structural proteins (ankyrin β, spectrin). Sclera included 18 collagen protein chains, small leucine-rich proteoglycans (decorin, biglycan, lumican, keratocan, prolargin, fibromodulin, mimecan), non-collagenous glycoproteins (fibronectin, vitronectin, laminin), extracellular matrix proteins (thrombospondins 1-4, dystroglycan, transgelins 1-3), and integrins alpha-V, alpha-1 and 2, beta-1, -2, and -5. Twenty-one unambiguous alternative splicing protein isoforms were identified in optic nerve and ten unambiguous alternative splicing protein isoforms were identified in sclera. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD001581.

show abstract

“…To reduce errors for ROI placement and perform more accurate tractography, we placed ROIs in the center of the annulus of Zinn, which is a confluence of the extraocular muscles, to facilitate the identification of the ON pathways. Age-related ON changes in various animals have been previously reported (35)(36)(37)(38)(39)(40). In summary, age-related changes may be classified into four: (i) axonal changes, (ii) myelin abnormalities, (iii) changes in neuroglial cells, and (iv) connective tissue thickening.…”

Section: Discussionmentioning

confidence: 85%

In vivo Analysis of Normal Optic Nerve in an Elderly Population Using Diffusion Magnetic Resonance Imaging Tractography

et al. 2021

View full text Add to dashboard Cite

Purpose: To quantitatively investigate the microstructural properties of the optic nerve (ON) in vivo using diffusion magnetic resonance imaging (dMRI) tractography in an elderly population and to determine the differences between the ON diffusion properties stratified by basic demographics.Methods: We measured fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD), and axial diffusivity (AD) of the intraorbital ON in cognitively normal controls selected from the Alzheimer's Disease Neuroimaging Initiative 3 database (n =104; mean age = 73. 8 ± 8.1 years) using dMRI probabilistic tractography and evaluated the correlation between diffusion parameters and demographic factors. Diffusion parameters were measured in 20 equidistant nodes along the tract, and the data from proximal 70% (14 nodes) of the intraorbital ON were averaged.Results: The mean FA of the intraorbital ON was 0.392 ± 0.063, and the mean MD was 1.163 ± 0.165 μm2/s. The mean RD was 0.882 ± 0.152 μm2/s, and the mean AD was 1.693 ± 0.183 μm2/s. The multiple linear regression model showed a negative correlation between FA and age. FA in females was significantly higher than males, whereas RD in female was significantly lower.Conclusions: We measured the diffusion properties of the intraorbital ON using dMRI tractography in an elderly cognitively normal population. The diffusion properties detected by dMRI tractography may substantially reflect the microstructure of the ON.

show abstract

Age-related changes in the optic nerve of Sprague-Dawley rats: An ultrastructural and immunohistochemical study

Cited by 8 publications

References 44 publications

Amacrine cells coupled to ganglion cells via gap junctions are highly vulnerable in glaucomatous mouse retinas

Amacrine cells coupled to ganglion cells via gap junctions are highly vulnerable in glaucomatous mouse retinas

The proteome of normal human retrobulbar optic nerve and sclera

In vivo Analysis of Normal Optic Nerve in an Elderly Population Using Diffusion Magnetic Resonance Imaging Tractography

Contact Info

Product

Resources

About