Age-related changes in the immunoreactivity of the monocyte/macrophage system in rat molar pulp

Okiji, Takashi; Kosaka, T; Kamal, Abu Mohammed Mostafa; Kawashima, Nobuyuki; Suda, Hiroshi

doi:10.1016/0003-9969(95)00146-8

Cited by 23 publications

(20 citation statements)

References 12 publications

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“…Recent immunohistochemical studies have disclosed that cells expressing macrophage-related phenotypes are distributed in a remarkably great density in the dental pulp of both the human (jontell et al, 1987;Bergenholtz et al, 1996;Okiji et al, 1997a) and the rat (lontell-et al, 1988;Okiji et al, 1992Okiji et al, , 1996Ohshima et al, 1994), and these cells appear to be the most predominant immune cells of the dental pulp. These studies have also unveiled that, in addition to the "histiocytes", cells with characteristics comparable with those of DCs do exist in the pulp.…”

Section: Macrophagesmentioning

confidence: 99%

Immune Defense Mechanisms of the Dental Pulp

Jontell

Okiji

Dahlgren

et al. 1998

Critical Reviews in Oral Biology & Medicine

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204

169

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Defense reactions of the dentin/pulp complex involve a variety of biological systems, in which the immune system plays a pivotal role. The knowledge of the organization and function of pulpal immunocompetent cells has been sparse, but in recent years a significant body of information of immune mechanisms in general has provided a footing for substantial new knowledge of the immune mechanisms of the dental pulp. The identification of pulpal dendritic cells (DCs) has generated research activities which have led to a concept of how an antigenic challenge may evoke a pulpal inflammatory response. Although DCs are not able to identify foreign antigens specifically, they provide necessary signals to activate T-lymphocytes which in turn will orchestrate other immunocompetent cells to mount the local immune defense of the dental pulp. The purpose of this review is to accent the organization and function of pulpal DCs and other tissue and cellular components and to provide a basis for how they may interact to instigate pulpal defense mechanisms.

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Section: Macrophagesmentioning

confidence: 99%

Immune Defense Mechanisms of the Dental Pulp

Jontell

Okiji

Dahlgren

et al. 1998

Critical Reviews in Oral Biology & Medicine

Self Cite

204

169

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“…OX6 ϩ , ED1-positive, or ED2-positive dendritic cells or macrophages are scarce in the incisor and molar pulp of newborn rats but increase in number after 2 weeks in the molar and 1 week in the incisor (Jontell et al 1991;Okiji et al 1996). Dendritic cells in the enamel organ of the maturation zone of rat incisors are scarce in juvenile animals, whereas numerous dendritic cells are present in the adult animals (Takano et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Presence of Anti-cystatin C–positive Dendritic Cells or Macrophages and Localization of Cysteine Proteases in the Apical Bud of the Enamel Organ in the Rat Incisor

Nishikawa

2005

J Histochem Cytochem.

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S U M M A R Y Cystatin C, a cysteine protease inhibitor, was examined in the apical buds of rat incisors by immunohistochemistry, because in transition and maturation zones most of the dendritic cells in the papillary layer are anti-cystatin C-positive. Anti-cystatin C-labeled cells were sparse and localized to the proliferation and differentiation zones, constituting the apical bud of 5-week-old rat incisors. These cells were considered macrophages or dendritic cells, based on their reactivity with OX6 and ED1, as well as their ultrastructure. Basement membrane at the periphery of apical bud was also labeled by anti-cystatin C antibody. The apical buds included a few apoptotic fragments and weak reactivity with antibody to cathepsin L, a cysteine protease. Reactivity to anti-cystatin C and anti-cathepsin L antibodies was also detected in the apical bud of newborn rat incisors. These results suggest that the cystatin C-positive macrophages or dendritic cells are involved in normal incisor formation. They may be related to the clearance of apoptotic cells or protection from putative cysteine protease activity.

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“…Dendritic cells show no or little activity of acid phosphatase (ACP), whereas macrophages have phagocytic capability and exhibit ACP activity that is dependent on the phagocytic capability (Steinman et al 1986). Class II MHC antigen-expressing cells have been identified in the dental pulp of humans and rats by immunohistochemistry using HLA-DR and OX-6 antibodies (Jontell et al 1987(Jontell et al , 1988(Jontell et al , 1991(Jontell et al , 1994Ohshima et al 1994Ohshima et al , 1995Ohshima et al , 1999Okiji et al 1994Okiji et al , 1996Okiji et al , 1997Yoshiba et al 1996). Based on the criteria of the expression of class II MHC antigens and the activity of ACP, previous studies have indicated that, whereas macrophages are present in the central region of the dental pulp, dendritic cells are located in and around the odontoblastic layer.…”

Section: Introductionmentioning

confidence: 99%

Appearance and distribution of dendritic cells and macrophages in dental pulp during early postnatal morphogenesis of mouse mandibular first molars

Tsuruga

Sakakura

Yajima

et al. 1999

Histochemistry and Cell Biology

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Dendritic cells and macrophages were examined in dental pulp during the postnatal development of mouse mandibular first molars, by immuno- and enzyme histochemistry. F4/80 antibody against dendritic cells and macrophages demonstrated labeled cells predominantly in and around the odontoblastic layer during tooth development from postnatal day 0 (PN0) to PN5. Labeling with Mac-1, Mac-2, and MOMA-2 antibodies against macrophages showed varied distribution patterns. Mac-1-positive cells were not detected in the dental pulp. Mac-2-positive cells appeared in the dental pulp at PN0, but not in or around the odontoblastic layer, and disappeared by PN3. A few MOMA-2-positive cells were detected in the dental pulp during the period examined. The F4/80-positive cells in and around the odontoblastic layer did not exhibit acid phosphatase or non-specific esterase activities. In addition, the F4/80-positive cells showed continued expression of Fcgamma receptor, but not class II major histocompatibility complex (MHC). Other antibodies against dendritic cells (NLDC-145, MIDC-8, and 33D1) did not label the F4/80-positive cells. We concluded that the F4/80-positive and class II MHC-negative cells in and around the odontoblastic layer may be immature dendritic cells in the early stages before eruption, weaning, and crucial exposure to antigenic stimuli. They may not only act primarily as immunosurveillance cells, but also play a role in a regulatory function and differentiation of odontoblasts.

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Age-related changes in the immunoreactivity of the monocyte/macrophage system in rat molar pulp

Cited by 23 publications

References 12 publications

Immune Defense Mechanisms of the Dental Pulp

Immune Defense Mechanisms of the Dental Pulp

Presence of Anti-cystatin C–positive Dendritic Cells or Macrophages and Localization of Cysteine Proteases in the Apical Bud of the Enamel Organ in the Rat Incisor

Appearance and distribution of dendritic cells and macrophages in dental pulp during early postnatal morphogenesis of mouse mandibular first molars

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