Age-related changes in reactive oxygen species production in rat brain homogenates

Driver, Amy S.; Kodavanti, Prasada Rao S.; Mundy, William R.

doi:10.1016/s0892-0362(99)00069-0

Cited by 313 publications

(155 citation statements)

References 49 publications

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“…The fluorescence was measured using Multimode plate reader (Thermo scientific, USA) with excitation at 480 nm and emission at 530 nm. Background fluorescence was corrected by the inclusion of parallel blanks, and data were expressed as pmol DCF formed/min/mg protein [15].…”

Section: Estimation Of Reactive Oxygen Species and Hydroperoxidesmentioning

confidence: 99%

Attenuation of adjuvant-induced arthritis by dietary sesamol via modulation of inflammatory mediators, extracellular matrix degrading enzymes and antioxidant status

et al. 2012

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Purpose The dietary sesamol is one of the important constituent of sesame seed that has been mainly claimed to combat cardiovascular disease and diabetes, which are the major secondary complications of arthritis. Thus, the present study was designed to evaluate the anti-arthritic, anti-inflammatory and anti-stress potentials of sesamol. Methods Arthritis was induced using Freund's complete adjuvant to hind paw of experimental rats. The physical and biochemical alterations and its recovery by sesamol were assessed by measuring enzymatic and non-enzymatic mediators. Arthritis-induced inflammation, oxidative stress and their protective by sesamol were measured by determining the levels of pro-inflammatory cytokines and oxidative stress markers. Results In the present study, sesamol was demonstrated to alleviate arthritis-induced cartilage degeneration by mitigating augmented serum levels of hyaluronidase and matrix metalloproteinases (MMP-13, MMP-3 and MMP-9). It also protected bone resorption by reducing the elevated levels of bone joint exoglycosidases, cathepsin D and tartarate-resistant acid phosphatases. Sesamol also abrogated the non-enzymatic inflammatory markers (TNF, IL1b, IL-6, COX-2, PGE 2 , ROS, and H 2 O 2 ,) effectively. In addition, sesamol neutralizes arthritis-induced oxidative stress by restoring the levels of reactive oxygen species, lipid and hydro peroxides and sustained antioxidant homeostasis by re-establishing altered activities of superoxide dismutase, catalase and glutathione-s-transferase. Conclusion Taken together, the study demonstrated the anti-arthritic, anti-inflammatory, anti-oxidative stress and chondro-protective potentials of sesamol in vivo. Thus, sesamol could be a single bullet that can fight arthritis as well as the secondary complications of arthritis such as cardio vascular disorders and diabetes.

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Section: Estimation Of Reactive Oxygen Species and Hydroperoxidesmentioning

confidence: 99%

Attenuation of adjuvant-induced arthritis by dietary sesamol via modulation of inflammatory mediators, extracellular matrix degrading enzymes and antioxidant status

et al. 2012

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“…ROS was measured based on the methods of Driver et al (2000) with slight modifications. Homogenates (20 ll) were pipetted into 96-well plates and allowed to warm to room temperature for 5 min.…”

Section: Ros Measurementmentioning

confidence: 99%

Effects of hexachlorobenzene on antioxidant status of liver and brain of common carp (Cyprinus carpio)

2006

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“…These findings could reflect the little known fact that changes in intrinsic antioxidant defense mechanisms accompany normal brain development (Nanda et al, 1996) and may contribute to the age-dependent brain sensitivity to oxidative stress (Driver et al, 2000). While further studies are needed to resolve this ambiguity, it may be speculated that the maturation of the antioxidant system occurs at different discrete points in time in males and females.…”

Section: Markers Of Oxidative Stress In the Hypergravity Exposed Rat mentioning

confidence: 97%

Potential role of oxidative stress in mediating the effect of altered gravity on the developing rat cerebellum

Sajdel-Sulkowska

Nguon

Sulkowski

et al. 2007

Advances in Space Research

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We have previously reported that perinatal exposure to hypergravity affects cerebellar structure and motor coordination in rat neonates. In the present study, we explored the hypothesis that exposure to hypergravity results in oxidative stress that may contribute to the decrease in Purkinje cell number and the impairment of motor coordination in hypergravity-exposed rat neonates. To test this hypothesis we compared cerebellar oxidative stress marker 3-nitrotyrosine (3-NT; an index of oxidative protein modification) and 8-hydroxy-2'-deoxyguanosine (8-OH-dG; an index of oxidative DNA damage) between stationary control (SC) and rat neonates exposed to 1.65 G (HG) on a 24-ft centrifuge from gestational day (G) 8 to P21. The levels of 3-NT and 8-OH-dG were determined by specific ELISAs. We also compared the Purkinje cell number (stereorologically) and rotarod performance between the two groups. The levels of 3-NT were increased only in HG females on P6 and on P12 in the cerebellum, and only in HG females on P12 in the extracellabellar tissue. Limited cerebellar data suggests an increase in the levels of 8-OH-dG on P12 only in HG females. In extracerebellar tissue the increase in 8-OH-dG levels was observed in both HG males and HG females except on P6 when it was only observed in HG males. While preliminary, these data suggest that the effect of hypergravity on the developing brain is sexdependent and may involve oxidative stress. Oxidative stress may, in turn, contribute to the decrease Purkinje cell number and impaired motor behavior observed in hypergravity-exposed rats.

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Age-related changes in reactive oxygen species production in rat brain homogenates

Cited by 313 publications

References 49 publications

Attenuation of adjuvant-induced arthritis by dietary sesamol via modulation of inflammatory mediators, extracellular matrix degrading enzymes and antioxidant status

Attenuation of adjuvant-induced arthritis by dietary sesamol via modulation of inflammatory mediators, extracellular matrix degrading enzymes and antioxidant status

Effects of hexachlorobenzene on antioxidant status of liver and brain of common carp (Cyprinus carpio)

Potential role of oxidative stress in mediating the effect of altered gravity on the developing rat cerebellum

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