Age-related Changes in Calbindin-D28k, Parvalbumin, and Calretinin Immunoreactivity in the Dog Main Olfactory Bulb

Abstract

Expression and age-related changes of calbindin-D28k (CB), parvalbumin (PV), and calretinin (CR) in the main olfactory bulb of the dog were investigated by immunohistochemistry and western blot analysis. Neurons that expressed these calcium-binding proteins showed a characteristic laminar distribution. Most of CB-immunoreactive neurons were observed in the glomerular layer (GL) and the inner sublayer of the external plexiform layer (EPL). Most of PV-immunoreactive neurons were observed in the outer sublayer of the EPL. CR-immunoreactive neurons were mainly distributed in the GL and the granule cell layer. With regard to age-related changes, CB-immunoreactive neurons in the GL were stable among all age groups; however, in the EPL they decreased with age. PV-immunoreactive neurons decreased in middle-aged and aged groups. However, CR-immunoreactive neurons were not decreased in middle-aged and aged groups. These results suggest that CB-immunoreactive neurons in the EPL were most sensitive to aging, and that their reduction may be related to aging in the dog.

Parvalbumin expression in the claustrum of the adult dog. An immunohistochemical and topographical study with comparative notes on the structure of the nucleus

“…An investigation in the dog prefrontal cortex revealed that there was no relationship between density of PV-ir neurons and either aging or neuropathological status (Pugliese et al, 2004). Choi et al (2010) reported in the dog main olfactory bulb an age related changes of calcium binding proteins. In particular, they showed a significant decrease of PV-ir neurons between young adult and middle-aged groups while a reduction was not seen between middle-aged and aged group.…”

<b>Caracterização morfológica e celular da zona subventricular e da corrente rostral migratória em encéfalos de fetos caninos</b>

“…Neste estudo, não foram encontradas células CALB-ir na CG do feto de cão, porém em cães adultos e roedores adultos é relatada a presença de interneurônios CALB-ir na CG CHOI et al, 2010), o que pode significar que os neurônios CALB-ir da CG aparecem numa idade mais tardia. Em um estudo usando 3 espécies de macacos adultos, também não foi encontrada marcação para a CALB na CG (ALONSO et al, 2001).…”

“…Na CPE de cães adultos é relatada a presença de um grande número de interneurônios CALB-ir que formam uma densa rede, cujo número de células decai conforme o cão envelhece, mas não foram encontradas células tufosas CALB-ir (CHOI et al, 2010). Também em roedores, foram encontrados neurônios CALB-ir na CPE .…”

“…A literatura demonstra que em cães adultos, foram encontradas poucas células CALR-ir nesta camada (CHOI et al, 2010).…”

“…Em cães adultos também foi encontrada uma grande quantidade de células CALR-ir na CCG (CHOI et al, 2010). O presente estudo demonstrou em feto de cães a presença dos neurônios CALR-ir em todas as camadas do BOP.…”