Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna

Mumtaz, Hammad; Dallas, Mark; Begonia, Mark T.; Lara-Castillo, Nuria; Scott, Jeannine M.; Johnson, Mark; Thiagarajan, Ganesh

doi:10.1016/j.bonr.2020.100266

Cited by 17 publications

(26 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most rodent studies use bending and torsion on long bones to mimic the typical loading modes in patients ( Fritton et al, 2000 ). Monotonic bending is a major whole-bone measurement of bone ductility and can be performed in three-point or four-point bending ( Liodaki et al, 2017 ; Zhang et al, 2017 ; Mumtaz et al, 2020 ; Zhang et al, 2020 ). While torsion tests are often used to evaluate fracture healing, fracture toughness tests ( Poundarik et al, 2012 ), time-dependent tests ( Lynch and Silva, 2008 ) and viscoelastic tests ( Maruyama et al, 2015 ) are also common in fracture studies, as they are more sensitive to bone matrix changes.…”

Section: Pre-clinical Measurements In Orthopedic Trauma: Connection Tmentioning

confidence: 99%

Modeling Complex Orthopedic Trauma in Rodents: Bone, Muscle and Nerve Injury and Healing

et al. 2021

View full text Add to dashboard Cite

Orthopedic injury can occur from a variety of causes including motor vehicle collision, battlefield injuries or even falls from standing. Persistent limb pain is common after orthopedic injury or surgery and presents a unique challenge, as the initiating event may result in polytrauma to bone, muscle, and peripheral nerves. It is imperative that we understand the tissue-specific and multicellular response to this unique type of injury in order to best develop targeted treatments that improve healing and regeneration. In this Mini Review we will first discuss current rodent models of orthopedic trauma/complex orthotrauma. In the second section, we will focus on bone-specific outcomes including imaging modalities, biomechanical testing and immunostaining for markers of bone healing/turnover. In the third section, we will discuss muscle-related pathology including outcome measures of fibrosis, muscle regeneration and tensile strength measurements. In the fourth section, we will discuss nervous system-related pathology including outcome measures of pain-like responses, both reflexive and non-reflexive. In all sections we will consider parallels between preclinical outcome measures and the functional and mechanistic findings of the human condition.

show abstract

Section: Pre-clinical Measurements In Orthopedic Trauma: Connection Tmentioning

confidence: 99%

Modeling Complex Orthopedic Trauma in Rodents: Bone, Muscle and Nerve Injury and Healing

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The formula for calculating the moment of inertia corresponding to the circular section of bone is given as follows (Mumtaz et al, 2020):…”

Section: Three Point Bending Failure Theory Of the Whole Bonementioning

confidence: 99%

“…The moment of inertia of hollow elliptical section is (Draper and Goodship, 2003 To calculate the ultimate bending stress, strain and flexural modulus according to the force-displacement curves, the maximum force in the force-displacement curve is defined as the failure point. Then the ultimate bending stress, bending strain and flexural modulus of the bone are respectively as follows (Cui and Liu, 1997;1998;Jungmann et al, 2011;Mumtaz et al, 2020):…”

Section: Three Point Bending Failure Theory Of the Whole Bonementioning

confidence: 99%

“…Flexural strength is one of the main testing index of bone mechanical properties to evaluate bone strength (Ayagara et al, 2019). Compared with the tensile strength, the flexural strength is simple and easy to obtain and the test success rate is high in the laboratory (Ferreño et al, 2017;Mumtaz et al, 2020). Bone bending test is divided into whole bone bending test and standard specimen bending test with the rectangular cross section.…”

Section: Introductionmentioning

confidence: 99%

“…Three-point bending test of the whole bone is one of biomechanical test methods for bone injury (Ridha and Thurner, 2013). The aim of this paper is to determine the influence of the loading rate on failure characteristics, model and parameters of the cattle bones with the same breed, age, nutritional condition and food structure (Mumtaz et al, 2020). Taking the bovine femur as a special research topic, the effect of loading rate on the characteristics of bovine bone and fracture is discussed by analysing the results of bending test.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Experimental Investigation on the Failure Mechanism of Bovine Bone by Three-point Bending Tests

Li¹,

Gao²,

Li³

et al. 2020

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

Bone damage is a common type of sport injury. The loading rate affects the type of fracture and the degree of soft tissue injury. In view of the problem of whole bone bending damage and failure, this paper introduces the test method of bovine bone bending and the mechanism of bending failure under different loading rates is analyzed through experimental research and theoretical analysis. The results of three-point bending failure test of bovine bone are given and the influence of loading rate on bending failure is discussed. The results show that there are three main deformation and failure modes of bovine bone under different loading rates, namely the mode of fracture failure under high loading rate, the mode of shear failure under medium loading rate and the mode of local wedge failure under low loading rate. With the increase of loading rate, the greater the failure force is applied on the bone, the smaller the destructive deflection of the bone will be. The maximum strain of bone decreases with the increase of loading rate, while the flexural modulus of bone increases gradually, which indicates that the loading rate has an effect on the brittleness of the whole bone. For the bending model of the whole bovine bone, the prediction result of elliptical cross-section method is more in accordance with the actual situation than that of circular cross-section method.

show abstract

A study of the influence of genetic variance and sex on the density and thickness of the calvarial bone in collaborative cross mice

Kaspersky,

Levy,

Nashef

et al. 2023

Anim Models and Exp Med

View full text Add to dashboard Cite

BackgroundBone microarchitecture is affected by multiple genes, each having a small effect on the external appearance. It is thus challenging to characterize the genes and their specific effect on bone thickness and porosity. The purpose of this study was to assess the heritability and the genetic variation effect, as well as the sex effect on the calvarial bone thickness (Ca.Th) and calvarial porosity (%PoV) using the Collaborative Cross (CC) mouse population.MethodsIn the study we examined the parietal bones of 56 mice from 9 lines of CC mice. Morphometric parameters were evaluated using microcomputed tomography (μCT) and included Ca.Th and %PoV. We then evaluated heritability, genetic versus environmental variance and the sex effect for these parameters.ResultsOur morphometric analysis showed that Ca.Th and %PoV are both significantly different among the CC lines with a broad sense heritability of 0.78 and 0.90, respectively. The sex effect within the lines was significant in line IL111 and showed higher values of Ca.Th and %PoV in females compared to males. In line IL19 there was a borderline sex effect in Ca.Th in which males showed higher values than females.ConclusionsThese results stress the complexity of sex and genotype interactions controlling Ca.Th and %PoV, as the skeletal sexual dimorphism was dependent on the genetic background. This study also shows that the CC population is a powerful tool for establishing the genetic effect on these traits.

show abstract

Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna

Cited by 17 publications

References 25 publications

Modeling Complex Orthopedic Trauma in Rodents: Bone, Muscle and Nerve Injury and Healing

Modeling Complex Orthopedic Trauma in Rodents: Bone, Muscle and Nerve Injury and Healing

Experimental Investigation on the Failure Mechanism of Bovine Bone by Three-point Bending Tests

A study of the influence of genetic variance and sex on the density and thickness of the calvarial bone in collaborative cross mice

Contact Info

Product

Resources

About