Age-related alterations in inflammatory response during experimental autoimmune prostatitis

Mast Cells and Ethanol Consumption: Interactions in the Prostate, Epididymis and Testis of UChB Rats

“…23,28 Unlike the prostate and epididymis, testicular mast cells have different location between humans and rodents. Interestingly, Moró n et al 24 In humans, the increased density of mast cells that occurs in the case of prostate cancer seems to be a protection against the tumorigenesis, 32 indicating a favorable tumor phenotype and prognosis. 29 In contrast to the ethanol, animals treated with estradiol in neonatal phase showed a large amount of mast cells in testicular interstitium because of the steroid direct action on the mast cells.…”

“…30,31 The normal prostate is a full microenvironment of mast cells, and several therapies with both estrogens and androgens do not alter this pattern. 24 Mast cells are evident in normal testes of humans and rodents. Interestingly, Moró n et al 24 In humans, the increased density of mast cells that occurs in the case of prostate cancer seems to be a protection against the tumorigenesis, 32 indicating a favorable tumor phenotype and prognosis.…”

“…These dynamics of mast cells were achieved by the absence or presence of metachromatic granules outside the mast cells in the surrounding connective tissue, as described by Moró n et al 24 and Keith et al 25 Sections were semiserial at 4 lm of thickness on the LEICA RM 2165 microtome. Because the granules within mast cells contain heparin and sulfated glycosaminoglycan, they were stained metachromatically using toluidine blue to evaluate and quantify the intact and degranulated mast cells.…”

Effect of experimentally induced diabetes on adult albino ratsʼ ventral prostate gland and role of selenium

“…Some researchers clarified that mast cells would facilitate the arrival and maintenance of infiltrating mononuclear cells in prostate through degranulation and releasing their mediators [41]. Other researchers [42,43] referred the presence of inflammatory cells in diabetic glands to high levels of glucose caused by diabetes that can block the correct function of immunological cells.…”

Testosterone Therapy Differently Regulates the Anti‐ and Pro‐Inflammatory Cytokines in the Plasma and Prostate of Rats Submitted to Chronic Ethanol Consumption (UChB)

“…Because the granules within mast cells contain heparin and sulfated glycosaminoglycan, they were stained metachromatically using toluidine blue to evaluate and quantify the intact and degranulated mast cells. These dynamics of mast cells were achieved by the absence or presence of metachromatic granules outside the mast cells in the surrounding connective tissue, as described by Moron et al 22 and Keith et al 23 The number of intact and degranulated mast cells was measured in five animals of each experimental group, and the values were given in cells/mm 2 . Random images of 50 histological fields per experimental group (10 images/rat) were captured by digital photomicroscope Axiophot II -Zeiss using 409 objective, with each field covering an area of 0.085 mm 2 .…”