Age determination of Aedes cantans using the ovarian oil injection technique

Hoc, T. Q.; Charlwood, Jacques Derek

doi:10.1111/j.1365-2915.1990.tb00281.x

Cited by 33 publications

(20 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Dissections for studying follicular dilatations were performed either on the same night of capture or on the following day, in order to decrease the number of recently oviposited females. In females that have recently oviposited, i.e., types A or B ovariolar stalks where encountered, Polovodova's technique can only be applied to follicles that have degenerated in an early stage of egg development [34]. Several of these non-functional follicles had to be visualized for a good estimate to be made.…”

Section: Methodsmentioning

confidence: 99%

Survivorship of Anopheles darlingi (Diptera: Culicidae) in Relation with Malaria Incidence in the Brazilian Amazon

2011

View full text Add to dashboard Cite

We performed a longitudinal study of adult survival of Anopheles darlingi, the most important vector in the Amazon, in a malarigenous frontier zone of Brazil. Survival rates were determined from both parous rates and multiparous dissections. Anopheles darlingi human biting rates, daily survival rates and expectation of life where higher in the dry season, as compared to the rainy season, and were correlated with malaria incidence. The biting density of mosquitoes that had survived long enough for completing at least one sporogonic cycle was related with the number of malaria cases by linear regression. Survival rates were the limiting factor explaining longitudinal variations in Plasmodium vivax malaria incidence and the association between adult mosquito survival and malaria was statistically significant by logistic regression (P<0.05). Survival rates were better correlated with malaria incidence than adult mosquito biting density. Mathematical modeling showed that P. falciparum and P. malariae were more vulnerable to changes in mosquito survival rates because of longer sporogonic cycle duration, as compared to P. vivax, which could account for the low prevalence of the former parasites observed in the study area. Population modeling also showed that the observed decreases in human biting rates in the wet season could be entirely explained by decreases in survival rates, suggesting that decreased breeding did not occur in the wet season, at the sites where adult mosquitoes were collected. For the first time in the literature, multivariate methods detected a statistically significant inverse relation (P<0.05) between the number of rainy days per month and daily survival rates, suggesting that rainfall may cause adult mortality.

show abstract

Section: Methodsmentioning

confidence: 99%

Survivorship of Anopheles darlingi (Diptera: Culicidae) in Relation with Malaria Incidence in the Brazilian Amazon

2011

View full text Add to dashboard Cite

show abstract

“…the rate of females which have had a blood meal and have laid eggs (as determined using the ovarian tracheole dissection by Detinova [81]). The reliability of this method, especially in older females is subject to discussion [82]. Age composition of female An.…”

Section: Introductionmentioning

confidence: 99%

Ecology of Anopheles darlingi Root with respect to vector importance: a review

Hiwat

Bretas²

2011

Parasites Vectors

169

194

View full text Add to dashboard Cite

Anopheles darlingi is one of the most important malaria vectors in the Americas. In this era of new tools and strategies for malaria and vector control it is essential to have knowledge on the ecology and behavior of vectors in order to evaluate appropriateness and impact of control measures. This paper aims to provide information on the importance, ecology and behavior of An. darlingi. It reviews publications that addressed ecological and behavioral aspects that are important to understand the role and importance of An. darlingi in the transmission of malaria throughout its area of distribution. The results show that Anopheles darlingi is especially important for malaria transmission in the Amazon region. Although numerous studies exist, many aspects determining the vectorial capacity of An. darlingi, i.e. its relation to seasons and environmental conditions, its gonotrophic cycle and longevity, and its feeding behavior and biting preferences, are still unknown. The vector shows a high degree of variability in behavioral traits. This makes it difficult to predict the impact of ongoing changes in the environment on the mosquito populations. Recent studies indicate a good ability of An. darlingi to adapt to environments modified by human development. This allows the vector to establish populations in areas where it previously did not exist or had been controlled to date. The behavioral variability of the vector, its adaptability, and our limited knowledge of these impede the establishment of effective control strategies. Increasing our knowledge of An. darlingi is necessary.

show abstract

“…The ovarian dissection technique, which is usually used for determination of the chronological age of mosquitoes, has some technical limitation such as the proportion of diagnostic ovarioles in the ovary declines with age and/or 1 1 1 2 2 2 2 2 2 2 3 3 3 3 3 too few diagnostic ovarioles may occur in some mosquito populations [22]. The current study was conducted to determine whether pteridine compounds were suitable for age estimation of A. stephensi.…”

Section: Discussionmentioning

confidence: 99%

Age-dependance of pteridines in the malaria vector, Anopheles stephensi

2017

View full text Add to dashboard Cite

Abstract:Determining the accurate age of malaria vectors is crucial to measure the risk of malaria transmission. A group of fluorescent chemicals derived from a pyrimidine-pyrazine ring structure known as pteridines from the head, thorax and whole body of adult female Anopheles stephensi were identified and evaluated as a tool for chronological and physiological age determination of malaria vectors. The female mosquitoes were collected from an insectary colony at an interval of every 5 days, up to 30 days, and the pteridines of head, thorax and whole body were detected fluorometrically by high-pressure liquid chromatography (HPLC) using excitation and emission wavelengths of 365 and 455 nm, respectively. In addition, alteration of the pteridines compounds was compared between blood and sugar fed mosquito groups. Although four pteridines including pterin-6-carboxylic acid, biopterin, xanthopterin and isoxanthopterin were detected, some of them were absent in the head or thorax of mosquitoes. Levels of all four pteridines were similarly decreased in a linear manner throughout 30 days. No significant difference in alteration of pteridine compounds was observed between the two groups of blood or sugar fed mosquitoes. This result indicates that diet has a little effect on pteridines alteration. Age determination based on pteridines, as an age-grading technique, could be used for field collected mosquitoes, which have either sugar or blood meal. In addition, analyzing total pteridine fluorescence from only whole body could be a convenient method to estimate the age.

show abstract

Age determination of Aedes cantans using the ovarian oil injection technique

Cited by 33 publications

References 8 publications

Survivorship of Anopheles darlingi (Diptera: Culicidae) in Relation with Malaria Incidence in the Brazilian Amazon

Survivorship of Anopheles darlingi (Diptera: Culicidae) in Relation with Malaria Incidence in the Brazilian Amazon

Ecology of Anopheles darlingi Root with respect to vector importance: a review

Age-dependance of pteridines in the malaria vector, Anopheles stephensi

Contact Info

Product

Resources

About