Age-Dependent Paraoxonase 1 (PON1) Activity and LDL Oxidation in Wistar Rats during Their Entire Lifespan

Kumar, Dinesh; Rizvi, Syed Ibrahim

doi:10.1155/2014/538049

Cited by 20 publications

(12 citation statements)

References 31 publications

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“…Additionally, analysis of PON-1 concentrations/activities with respect to age and BMI, as factors that can affect PON-1 synthesis, showed an inverse relation of these parameters to age in the group of AP patients. It remains in accordance with the results of the study conducted by Kumar et al, in which a strong relation of age and PON-1 activity was shown 41. It was noted that age was strongly related to the increase of blood level of lipid peroxidation markers, which could impair PON‑1 function as antioxidant or its synthesis dependent on lipoprotein metabolism 41.…”

Section: Discussionsupporting

confidence: 90%

Decreases in Paraoxonase-1 Activities Promote a Pro-inflammatory Effect of Lipids Peroxidation Products in Non-smoking and Smoking Patients with Acute Pancreatitis

et al. 2018

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Aim: The study investigated the extent to which tobacco smoke exposure causes changes in lipids biochemistry through measurement blood concentrations of: paraoxonase-1 (PON-1) activities as lipid-bound enzyme into cell membrane, concentration of malonyldialdehyde (MDA), protein adducts of 4-hydroxynonenal (HNE-adducts), oxidized low density lipoproteins (oxLDL), total cholesterol (CH) and high-density lipoprotein cholesterol (HDL). Additionally, the activity of P isoform of glutathione S-transferase (GST-π) was measured.Methods: Investigations were performed in the blood of patients with acute pancreatitis (AP) on the 1st, 3rd and 7th day of hospitalization and in healthy volunteers. The activities of PON-1 forms, GST-π were determined spectrophotometrically. Concentrations of PON-1, MDA, HNE-adducts, oxLDL, HDL, CH were measured using commercial tests.Results: Near 2-fold higher concentrations of MDA, HNE-adducts, oxLDL, correlating with inflammatory markers in AP patients compared to healthy subjects were demonstrated, which were accompanied by gradually increasing CH/HDL ratio during hospitalization. During hospital treatment, decreased activities of all PON-1 subtypes were observed in AP patients compared to healthy subjects, more pronounced in tobacco smokers. A decreased PON-1 phosphotriesterase activity in non-AP control group smokers compared to non-smokers was noted. In non-smoking AP patients GST-π activity normalized during hospitalization in contrast to smokers.Conclusions: GST-π and PON-1 phosphotriesterase activities seem to be a sensitive marker of pro/antioxidative imbalance in smokers. Lipids peroxidation products generated during AP can intensify preexisting inflammation. Increasing stay in the hospital was associated with worsening of lipids peroxidation markers and the parameters of lipid profile, in both non-smoking and smoking AP patients, what can indicate that the oxidative-inflammatory process are not extinguished.

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Section: Discussionsupporting

confidence: 90%

Decreases in Paraoxonase-1 Activities Promote a Pro-inflammatory Effect of Lipids Peroxidation Products in Non-smoking and Smoking Patients with Acute Pancreatitis

et al. 2018

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“…Lower levels of PON1 in C57BL/6J mice have been reported to result in lower levels of lipid metabolism intermediates, in comparison to suggesting a decrease in lipolysis and decreased fatty acid oxidation . A few reports show the role of polyphenols to stimulate PON1 . The maintenance of the enzyme‐SH group justifies the importance of plasma redox status as an important factor in the modulation of PON1 activity .…”

Section: Discussionmentioning

confidence: 99%

“…43 A few reports show the role of polyphenols to stimulate PON1. 44 The maintenance of the enzyme-SH group justifies the importance of plasma redox status as an important factor in the modulation of PON1 activity. 45 Our observation of hesperidin supplementation reversing the effect of hyperlipidaemic stress in atherogenic supplemented rats highlights the antioxidant role of hesperidin in protecting the -SH group of PON 1.…”

Section: Discussionmentioning

confidence: 99%

Hesperidin attenuates altered redox homeostasis in an experimental hyperlipidaemic model of rat

Kumar

Akhtar

Rizvi

2020

Clin Exp Pharma Physio

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Diets rich in saturated fats and cholesterol contribute to the incidence of hyperlipidaemia. An altered lipid profile is a major factor responsible for the development of CVD. Male Wistar rats were fed with a high‐fat diet (HFD) (suspension (w/v) of 0.5% cholesterol, 3% coconut oil and 0.25% cholic acid for 30 days) to induce an experimental hyperlipidaemic model. High‐fat diet fed rats were also supplemented with hesperidin (100 mg/kg body weight). The present study reports reactive oxygen species (ROS) production, oxidative stress parameters: malondialdehyde (MDA), protein carbonyl (PCO), oxidation of plasma protein (AOPP), and advance glycation end products (AGEs); antioxidant defence parameters: ferric reducing ability of plasma (FRAP), reduced glutathione (GSH), Paraoxonase‐1 (PON‐1), plasma membrane redox system (PMRS); general biochemical parameters: triglyceride, cholesterol, serum glutamic oxaloacetic transaminase (SGOT), and serum glutamic pyruvic transaminase (SGPT), fasting insulin, fasting glucose, homeostatic model assessment–insulin resistance (Homa‐IR) index, and inflammatory biomarkers: interleukin (IL)‐6 and tumour necrosis factor (TNF)‐α. Experimental hyperlipidaemia was found to be associated with significantly higher body weight (27.58%), cholesterol (140%), triglyceride (190%), and fasting glucose level (37%). Reactive oxygen species production (67%), MDA (28.9%), AOPP (31.42%), PCO (58.53%), and PMRS (156%), inflammatory markers, cytokines IL‐6 and TNF‐α, were elevated and GSH (50%), PON 1 (37.07%), and FRAP (26.58%) activity were significantly (P < .05) lower in the high‐fat diet group. Hesperidin supplementation protected HFD‐fed rats from oxidative damage. Our findings indicate that the supplementation of hesperidin provides protection against redox imbalance induced by hyperlipidaemia in rats.

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“…did not observe such a reduction in children [ 9 , 10 ]. This may be due to the decrease of enzyme activity with age [ 11 ]. Our results are consistent with those of Aslan et al .…”

Section: Discussionmentioning

confidence: 99%

Serum paraoxonase 1 activity in patients with iron deficiency anemia

Okuturlar¹,

Gedikbaşı²,

Akalın³

et al. 2016

aoms

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IntroductionIn this study we aimed to detect paraoxonase 1 (PON-1) activity in iron deficiency anemia (IDA) and to compare it with healthy controls by observing the change after iron therapy.Material and methodsIn this study, 50 adult patients with IDA and 40 healthy subjects were enrolled. All patients were analyzed at the beginning and after treatment according to laboratory assessments.ResultsMean paraoxonase and arylesterase activities in the iron deficiency anemia group were significantly lower than mean activities of the control group (102.4 ±19.2 U/l and 163.3 ±13.68 U/l, respectively and 157.3 ±26.4 U/l and 256.1 ±24.6 U/l, respectively; p = 0.0001 for both). Paraoxonase and arylesterase activities significantly increased after treatment for IDA (143.2 ±13.9 and 197.6 ±27.9 U/l, respectively, p = 0.0001). Mean activities after treatment with iron were significantly lower than mean activities in the control group (p = 0.002; p = 0.0001 respectively).ConclusionsParaoxonase and arylesterase activities in patients with IDA significantly increased after treatment with iron therapy. In adults IDA may also be one of the factors associated with increased risk of atherosclerosis.

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Age-Dependent Paraoxonase 1 (PON1) Activity and LDL Oxidation in Wistar Rats during Their Entire Lifespan

Cited by 20 publications

References 31 publications

Decreases in Paraoxonase-1 Activities Promote a Pro-inflammatory Effect of Lipids Peroxidation Products in Non-smoking and Smoking Patients with Acute Pancreatitis

Decreases in Paraoxonase-1 Activities Promote a Pro-inflammatory Effect of Lipids Peroxidation Products in Non-smoking and Smoking Patients with Acute Pancreatitis

Hesperidin attenuates altered redox homeostasis in an experimental hyperlipidaemic model of rat

Serum paraoxonase 1 activity in patients with iron deficiency anemia

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