Age-dependent frequency of unconventional T cells in a healthy adult Caucasian population: a combinational study of invariant natural killer T cells, γδ T cells, and mucosa-associated invariant T cells

Singh, Parvind; Száraz-Széles, Marianna; Mezei, J. Zs.; Baráth, Sándor; Hevessy, Zsuzsanna

doi:10.1007/s11357-022-00515-5

Cited by 15 publications

(14 citation statements)

References 71 publications

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“…Even without stim, the accumulation of gd T cells were found to be higher in adult compared with newborn (~2.5 fold) and elder (~3 fold, Figure 1B). Age associated alterations in γδ T cell compartment are well studied [71][72][73] . γδ T cells showed an association during viral infections like Cytomegalovirus 74,75 , influenza 76 or COVID-19 [77][78][79] .…”

Section: Discussionmentioning

confidence: 99%

Immune profiling of age and adjuvant-specific activation of human blood mononuclear cells in vitro

Dowling,

Schüller,

Barman

et al. 2023

Preprint

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Vaccination reduces morbidity and mortality due to infections, but efficacy may be limited due to distinct immunogenicity at the extremes of age. This raises the possibility of employing adjuvants to enhance immunogenicity and protection. Early IFNγ production is a hallmark of effective vaccine immunogenicity in adults serving as a biomarker that may predict effective adjuvanticity. We utilized mass cytometry (CyTOF) to dissect the source of adjuvant-induced cytokine production in human blood mononuclear cells (BMCs) from newborns (~39-week-gestation), adults (~18-63 years old) and elders (>65 years of age) after stimulation with pattern recognition receptors agonist (PRRa) adjuvants. Dimensionality reduction analysis of CyTOF data mapped the BMC compartment, elucidated age-specific immune responses and profiled PRR-mediated activation of monocytes and DCs upon adjuvant stimulation. Furthermore, we demonstrated PRRa adjuvants mediated innate IFNγ induction and mapped NK cells as the key source of TLR7/8 agonist (TLR7/8a) specific innate IFNγ responses. Hierarchical clustering analysis revealed age and TLR7/8a-specific accumulation of innate IFNγ producing γδ T cells. Our study demonstrates the application of mass cytometry and cutting-edge computational approaches to characterize immune responses across immunologically distinct age groups and may inform identification of the bespoke adjuvantation systems tailored to enhance immunity in distinct vulnerable populations.

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Section: Discussionmentioning

confidence: 99%

Immune profiling of age and adjuvant-specific activation of human blood mononuclear cells in vitro

Dowling,

Schüller,

Barman

et al. 2023

Preprint

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“…Aging leads to an involution of the function of the immune system which is essentially linked to a lower replicative response to antigenic stimulation and to a decrease in the function of natural killer lymphocyte cells [122][123][124]. Furthermore, with age, we witness the physiological involution of the thymus with a consequent numerical and functional reduction in T-lymphocytes [125,126].…”

Section: Changing In Physiological and Immune Status In The Elderlymentioning

confidence: 94%

Clustering Disease of Clostridioides Difficile Infection: Implication for the Management in Internal Medicine

Crispino

2023

Microbiology Research

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Clostridioides difficile is a bacterium responsible for a healthcare-associated gastrointestinal infection, primarily affecting people who have undergone prolonged antibiotic treatment or who have compromised immune systems. The CD is of particular concern due to its high recurrence rates and the potential for serious outcomes, including life-threatening conditions such as pseudomembranous colitis, septic shock, and all associated conditions. Since this infection is a disease associated with other health conditions, a general vision of the problems is necessary which aims to obtain a general overview of the manifestations that generally correlate with care. Clinical reasoning following the disease-clustering method is able to produce a categorization process by grouping the possible correlations of the various conditions or factors underlying diseases on the basis of certain similarities or common models. The clustering process is performed using data analysis techniques which, by statically correlating each other, give an exact dimension of all the information related to a particular disease. In the case of CD, reasoning based on disease clustering has better clarified the practices, appropriateness in infection control, judicious use of antibiotics, and research into therapeutic and preventive strategies. This review, taking advantage of the clustering strategy, aimed to analyze the contingent conditions of the infection under examination, to reduce the incidence and impact of CD, having as its mission the improvement of the results deriving from the contrast of all those correlated pathological conditions to healthcare for the improvement of public health.

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“…However, they share some common upregulated and downregulated genes that are different from those in conventional T cells and might provide a clue concerning the relationship between these cells [ 7 ]. MAIT cells recognize the microbial riboflavin-derivative antigens that are presented by the nonpolymorphic MHC class I-like protein MR1 [ 8 ] and are abundant in the liver, representing up to 45% of liver T cells [ 9 ] and up to 10% of T cells in peripheral blood (PB) [ 10 ]; no significant difference was found between sex (male and female) [ 11 ]. The roles of Vα7.2 + /CD161 − T cells are not clearly understood.…”

Section: Introductionmentioning

confidence: 99%

Investigating Vα7.2+/CD161− T Cell and MAIT Cell Profiles Using Flow Cytometry in Healthy Subjects and Subjects with Atopic Dermatitis

Singh,

Gaspar,

Szegedi

et al. 2024

IJMS

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This study investigates the roles of mucosal-associated invariant T (MAIT) cells and Vα7.2+/CD161− T cells in skin diseases, focusing on atopic dermatitis. MAIT cells, crucial for bridging innate and adaptive immunity, were analyzed alongside Vα7.2+/CD161− T cells in peripheral blood samples from 14 atopic dermatitis patients and 10 healthy controls. Flow cytometry and machine learning algorithms were employed for a comprehensive analysis. The results indicate a significant decrease in MAIT cells and CD69 subsets in atopic dermatitis, coupled with elevated CD38 and polyfunctional MAIT cells producing TNFα and Granzyme B (TNFα+/GzB+). Vα7.2+/CD161− T cells in atopic dermatitis exhibited a decrease in CD8 and IFNγ-producing subsets but an increase in CD38 activated and IL-22-producing subsets. These results highlight the distinctive features of MAIT cells and Vα7.2+/CD161− T cells and their different roles in the pathogenesis of atopic dermatitis and provide insights into their potential roles in immune-mediated skin diseases.

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Age-dependent frequency of unconventional T cells in a healthy adult Caucasian population: a combinational study of invariant natural killer T cells, γδ T cells, and mucosa-associated invariant T cells

Cited by 15 publications

References 71 publications

Immune profiling of age and adjuvant-specific activation of human blood mononuclear cells in vitro

Immune profiling of age and adjuvant-specific activation of human blood mononuclear cells in vitro

Clustering Disease of Clostridioides Difficile Infection: Implication for the Management in Internal Medicine

Investigating Vα7.2+/CD161− T Cell and MAIT Cell Profiles Using Flow Cytometry in Healthy Subjects and Subjects with Atopic Dermatitis

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