Age-dependent Degradation of the Protein Adsorption Capacity of Titanium

Hori, Norio; Att, Wael; Ueno, Takeshi; Sato, Nobuaki; Yamada, Masahiro; Saruwatari, Lei; Suzuki, Takeo; Ogawa, Takahiro

doi:10.1177/0022034509339567

Cited by 120 publications

(108 citation statements)

References 19 publications

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“…This leads to an increase in hydrophobicity, which is considered an aging phenomenon 24,25) . It is also reported that the atomic percentage of carbon reaches 50 at% on hydrophobic titanium surfaces stored in air atmosphere 18,26) . The previous study showed that the specimens stored in air atmosphere more than 1 week had hydrophobicity 5) .…”

Section: Discussionmentioning

confidence: 94%

Influence of various superhydrophilic treatments of titanium on the initial attachment, proliferation, and differentiation of osteoblast-like cells

et al. 2015

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The purpose of this study was to investigate the influence of superhydrophilic treatments of titanium on the behavior of osteoblastlike cells. Superhydrophilic specimens were prepared with sandblast and acid-etching (DW), oxygen plasma (Plasma) and ultraviolet light (UV), and were stored in distilled water for 3 days immediately after these treatments. Specimens stored in air for 3 weeks were used as a control Air group. Initial cell attachment, proliferation, alkaline phosphatase activity, and osteocalcin secretion of mouse osteoblast-like cells MC3T3-E1 were enhanced more on superhydrophilic groups than were Air specimens. On confocal laser scanning microscope images of cell morphology, the expression of actin filaments was observed on the superhydrophilic groups, whereas relatively little actin filament expression was seen on the Air surfaces on all culture periods. These results indicate that DW, Plasma, or UV treatment has potential for the creation and maintenance of superhydrophilic surfaces and the enhancement of the initial attachment, proliferation, and differentiation of osteoblast-like cells.

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Section: Discussionmentioning

confidence: 94%

Influence of various superhydrophilic treatments of titanium on the initial attachment, proliferation, and differentiation of osteoblast-like cells

et al. 2015

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“…A series of studies revealed that osteoblast-affinity to titanium implants decreases over time. Furthermore, an implant biomechanical test demonstrated that the biomechanical strength of the bone-titanium integration for 4-week-old implants was less than half that for newly processed implants during the early healing stage at week 2 9,22) . This "aging-like change" of the biological potential of titanium may be one of the reasons why the currently used titanium implants never achieve the ideal complete osseointegration.…”

Section: Discussionmentioning

confidence: 99%

NaOCl-mediated biofunctionalization enhances bone-titanium integration

et al. 2015

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The aim of this study was to examine the effect of NaOCl pretreatment on the biomechanical fixation of implant at the early healing stage of a rat model. Polished titanium cylindrical implants and disks were prepared, and one-half of these samples were dual acidetched. Then, one-half of both surfaces were chemically-cleaned by pretreatment with 5% NaOCl solution for 24 h. Morphological analyses showed that there was no significant difference between before and after NaOCl treatment. The wettability measurement demonstrated that NaOCl treatment secondarily converted both titanium surfaces from hydrophobic to superhydrophilic, accompanied by the removal of hydrocarbons from the titanium surfaces. Biomechanical push-in test indicated that the bone-titanium integration strength of the NaOCl-treated implants were significantly greater than that of the untreated implants (p<0.05). These results showed that NaOCl pretreatment enhanced the osseointegration capability of titanium, indicating its potential for a simple chemical chair-side pretreatment method.

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“…Acta Biomater Our previous studies demonstrated an increase in the occupancy of carbon and a reduction in osteoblast attachment and protein absorption on bulk titanium surfaces over storage-time in a sterile atmosphere after surface preparation including machining and acid-etching, which was termed the "biological aging of titanium" [32][33][34]. This time-dependent degradation of biological activity on the titanium surface was statistically correlated with an increase in the occupancy of carbon.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

et al. 2010

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Ultraviolet (UV)-photofunctionalization of titanium substantially enhances the strength and quality of osseointegration by promoting osteogenic cellular attachment and proliferation. However, the mechanism underlying the initial interaction between the cells and the surface of the material remains to be elucidated, especially where the influence of surface roughness is excluded as a factor.We evaluated the effect of UV-photofunctionalization on the adhesive strength and cellular stiffness of a single osteoblast and its association with extent of cell spread, cytoskeletal development and focal adhesion assembly on a very smooth titanium surface. Rat bone marrow-derived osteoblasts were cultured on UV-treated or -untreated mirror-polished titanium disks. The mean critical shear force required to initiate detachment of a single osteoblast (n = 10) was over 2000 nN on a UV-treated surface at 3 hr incubation, which was 17 times greater than that on an untreated surface.The mean total energy required to complete the detachment of osteoblasts (n = 10) was consistently over 60 pJ on a UV-treated titanium surface after 24 hr culture, which was up to 42 times greater than that on an untreated surface. Cellular shear modulus, which represents cellular stiffness, was consistently greater on a UV-treated surface than on an untreated surface after 24 hr incubation (n = 10). This strengthening of cell adhesion and cellular mechanical properties on UV-treated titanium was accompanied by enhanced cell spread and actin fiber development and increased levels of vinculin expression. These results indicate that UV-photofunctionalization substantially strengthens osteoblast retention on titanium bulk material with no topographical features and that this is associated with enhancement of intracellular structural development during the cell adhesion process.

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Age-dependent Degradation of the Protein Adsorption Capacity of Titanium

Cited by 120 publications

References 19 publications

Influence of various superhydrophilic treatments of titanium on the initial attachment, proliferation, and differentiation of osteoblast-like cells

Influence of various superhydrophilic treatments of titanium on the initial attachment, proliferation, and differentiation of osteoblast-like cells

NaOCl-mediated biofunctionalization enhances bone-titanium integration

Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

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