Age-Dependent Changes in Glutathione-S-Transferase: Correlation with Total Plasma Antioxidant Potential and Red Cell Intracellular Glutathione

Abstract

The correlation between antioxidant capacity and oxidative damage during aging has been reported in several tissues in different species. Glutathione-S-transferases (GST) can metabolise endogenous and exogenous toxins and carcinogens by catalysing the conjugation of diverse electrophiles with reduced glutathione (GSH). We observe a significant (P < 0.001) increase in plasma GST activity as a function of human age (r = 0.5675). A significant (P < 0.001) positive correlation (r = 0.8979) is observed between GST activity and total plasma antioxidant potential measured as ferric reducing ability of the plasma (FRAP). GST activity and red cell intracellular GSH also show a significant positive correlation (r = 0.7014). We hypothesize that the increased activity of plasma GST is a manifestation of increased generation of ROS and a concomitant decrease in the level of plasma antioxidant capacity during aging.

Oxidative stress and thiols depletion impair tibia fracture healing in young men with type 2 diabetes

“…Increased level of GST seen in DTF persons with diabetes could be a manifestation of increased ROS generation as a result of prolonged hyperglycemia as well as a significant decreasing in GSH in mentioned group [48]. The correlation analysis revealed that GST and GSH are in a reverse correlation (r = -0.67, P < 0.01), which is in accord with previous studies [48,49]. Obviously, GST is highly likely to have a protective role in patients with diabetes after a tibia fracture but the increase in GST would not be sufficient enough to tackle ROS.…”

Association of glutathione-S-transferase with patients of type 2 diabetes mellitus with and without nephropathy

“…The correlation analysis revealed that GST and GSH are negatively correlated which is in sync with a previous study by Maurya and Rizvi 2010 [31]. As for the correlation of GST, OS markers and hyperglycemia, HbA1c is positively associated with increase in the activity of GST and MDA.…”

Age-Related Changes in Hepatic Activity and Expression of Detoxification Enzymes in Male Rats

“…On the other hand, elevation in liver and brain GST activities in male Wistar rats during aging have been reported by Kim et al [50] and Oztürk and Gümüşlü [51]. Also increase in plasma GST activity as a function of human age was described [52]. Elevation in GST activity/expression observed in this study could be explained as a compensatory mechanism against increased formation of oxidative stress products (e.g., organic hydroperoxides) accompanying process of aging.…”