Age changes of bones and joints in various strains of mice

Silberberg, Martin; Silberberg, Ruth

doi:10.1002/aja.1000680104

Cited by 72 publications

(27 citation statements)

References 13 publications

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“…Table 6 summarises the main findings from x-rays taken at 3 ages. Epiphyseal fusion is a slow process in rodents and difficult to detect even histologically (Silberberg & Silberberg, 1941). The olecranon process gave fewest doubtful results and showed an increase in epiphyses fused with age.…”

Section: Organ Weightsmentioning

confidence: 99%

Lifespan, age changes and tumour incidence in an ageing C57BL mouse colony

1976

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Detailed data from a colony of C57BL mice kept for research into ageing processes are given on weights of 13 organs between the ages of 6 and 30 months; pathological findings and x-ray results from animals aged 6, 24 and 30 months; age-specific tumour incidences in 24 organs in 3-month period up to 36 months of age. Results of previously published age-related studies of reproductive capacity, dental abnormalities and brain changes are summarised.

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Section: Organ Weightsmentioning

confidence: 99%

Lifespan, age changes and tumour incidence in an ageing C57BL mouse colony

1976

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“…The changes in the developing articular cartilage are referred to as the maturation process and are determined genetically, endocrinologically, by nutritional conditions (Silberger and Silberger 1941;Silberger et at. 1961) and, particularly in the postnatal period, by endogenous environmental factors (Ghadially 1978(Ghadially , 1981(Ghadially , 1982Perrin et al 1978).…”

Section: Ultrastructure Porcine Articular Cartilage Developmentmentioning

confidence: 99%

The Ultrastructure of Articular Cartilage in the Prenatal Pig

Horký¹

1989

Acta Vet. Brno

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Horky D.: The Ultrastructure of Articular Cartilage in the PrenatalPig. Acta vet. Brno, 58,1989: 143-174.The articular cartilage of the femoral articulation was studied in 14 porcine foetuses of both &exes at 41, 61 and 94 days after fertilization. The structure of articular chondroblasts and intercellular matter enabled us to distinguish three zones as early as at 41 days. The surface zone contained cells at a low degree of differentiation in structure resembling fibroblasts. The medium zone showed thick bundles of collagen microfibrils penetrating through the cell membrane into the intercellular matter. At 61 days both the chondroblast cytoplasm and the cartilage surface of the medium zone had vesicles, several !-10m in size, bounded by a single membrane. At 91 days after fertilization the cytoplasm of chondroblasts found at the border between the surface and the medium zones revealed collagen microfibrils running through cell memblanes. The fibrils showed signs of periodicity.The surface layer of the cartilage in the period under study was made up largerly of aperiodic fibrils. In some regions below the surface bundles of collagen microfibrils ran parallel to the surface of the articular cartilage. They later became involved into the chondrosynovial membrane. Ultrastructure, porcine articular cartilage, developmentIn the course of articular cartilage development both chondrocytes and intercellular matter are subject to differentiation processes which, in the prenatal period, lead to the formation of articular cartilage followed by further changes in the two components in the neonatal period. The changes in the developing articular cartilage are referred to as the maturation process and are determined genetically, endocrinologically, by nutritional conditions (Silberger and Silberger 1941;Silberger et at. 1961) and, particularly in the postnatal period, by endogenous environmental factors (Ghadially 1978(Ghadially , 1981(Ghadially , 1982Perrin et al. 1978). Changes in morphology of articular cartilage are associated, to a major extent, with age (except for species-related changes). Such changes have been reported for instance in adult mice (Silberger et al. 1976), rabbits (Barnett et al. 1963; Davies et al.

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“…Alterations in the chemical composition of the latter constitute one of the earliest changes noticed in age-related osteoarthritis (7-1 1). Age-related degenerative changes in articular cartilages (2,9,12-16) which precede osteoarthritis (17)(18)(19) resemble, to a great extent, those changes observed in the joints of animals submitted to glucocorticoid treatment (20).…”

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confidence: 98%

Cartilage surface charge. A possible determinant in aging and osteoarthritic processes

Laver-Rudich

Silbermann

1985

Arthritis & Rheumatism

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Polycationic labels such as cationized ferritin and colloidal iron were used to evaluate the surface negative charges over the mandibular condyles of ICR mice. The effects of neuraminidase, hyaluronidase, pronase, and collagenase on the binding of cationized ferritin and colloidal iron particles to the condylar articular surface were also studied. The results of this study clearly indicate that the surface area of the cartilaginous condyle is negatively charged and that its composition consists mainly of a collagenous material embedded within a proteinaceous matrix. With age, a substantial decrease in the density of negative charges took place along the surface area and, in particular, in the context of sialic acid residues. It is, therefore, possible that the reduction in cartilage surface charge might be associated with the onset of osteoarthritic changes commonly seen in aging humans and experimental animals.The articular portion of condylar cartilage differs in its structure and function from the rest of the organ and plays a special role in joint biomechanics and cartilage nutrition (1-6). Alterations in the chemical composition of the latter constitute one of the earliest changes noticed in age-related osteoarthritis (7-1 1). Age-related degenerative changes in articular cartilages (2,9,12-16) which precede osteoarthritis (17-19) resemble, to a great extent, those changes observed in the joints of animals submitted to glucocorticoid treatment (20).Previous studies have indicated a close correlation between age-related changes in cartilage and the alterations in the glycosaminoglycan content of this tissue (2,4,21,22). Polycationic labels such as the cationized ferritin (CF), which labels all anionic groups at physiologic pH (23), and colloidal iron (GI) hydroxide particles, which normally label only the carboxylic group of sialic acids at pH 1.8 (24), have been used to study changes in the chemical nature of articular cartilages (6,25).In the present study, we applied the cationized ferritin and colloidal iron labeling techniques and used a variety of enzymes, such as neuroaminidase, hyaluronidase, pronase, and collagenase, to evaluate the age-related changes in the charge properties of the articular cartilage. MATERIALS AND METHODSMice. Pregnant ICR mice were obtained from the Animal Breeding Center of the Weizmann Institute of Science (Rehovot, Israel), and the offspring (male and female) were maintained in our laboratory up to the age of 12 months. One hundred fifteen animals were used; the offspring were divided into 3 experimental groups: 50 neonatal mice (5-7 days old), 40 young-adult animals (2-3 months old), and 25 older mice (12 months old).Mandibular condyles (2 from each animal) were obtained while the animals were anesthetized with ether. All

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Age changes of bones and joints in various strains of mice

Cited by 72 publications

References 13 publications

Lifespan, age changes and tumour incidence in an ageing C57BL mouse colony

Lifespan, age changes and tumour incidence in an ageing C57BL mouse colony

The Ultrastructure of Articular Cartilage in the Prenatal Pig

Cartilage surface charge. A possible determinant in aging and osteoarthritic processes

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