Age-associated aberrations in mouse cellular and humoral immune responses

Abstract

A decline in cell-mediated immune response, chronic inflammation and aggravation of humoral immunity was evident which conclusively suggests a skewed Th2 pathway during aging.

Improvement in Th1/Th2 immune homeostasis, antioxidative status and resistance to pathogenic E. coli on consumption of probiotic Lactobacillus rhamnosus fermented milk in aging mice

“…Data were subjected to two-way analysis of variance (ANOVA) and the Bonferroni test was used to separate the means (P<0.05) which were considered statistically significant. All immunological data are supplemented with respective data of 4 months old young animals (as per Sharma et al 2013b) for better inference of probiotic effects.…”

“…At the end of their respective study period, animals from each set were sacrificed by diethyl ether overdose and blood, peritoneal fluid, spleen, intestine, and liver of the animals were collected to assess various immunological parameters. In order to gain a better understanding of probiotic anti-immunosenescence effects in aging animals (16 months old), all immunological results presented in current investigation have also been supplemented with respective normal data of young (4 months old) animals as earlier investigated in our laboratory (Sharma et al 2013b).…”

“…Considering these aspects, we initially characterized age-associated changes in immune system of male Swiss albino mice (Sharma et al 2013b) and observed imbalance in Th1/Th2 immune pathways along with variations in cellular and humoral immunological functions. Based on this prior knowledge, the present study was designed to assess anti-immunosenescence effects of consumption of milk fermented with probiotic Lactobacillus rhamnosus (LR; MTCC 5897) in aging mice employing several markers of cellular and humoral immunosenescence as well as antioxidative capacity.…”

Ovarian kisspeptin expression is related to age and to monocyte chemoattractant protein-1

“…One of the inflammatory molecules that plays a role in the human ovulatory process and relates to both aging and obesity [14][15][16][17][18][19] is monocyte chemoattractant protein-1 (MCP-1) [20]. MCP-1, a polypeptide composed of 76 amino acids, is produced by various cell types including endothelial cells, fibroblasts, tumor cells, monocytes, and macrophages [20,21].…”

“…In humans, physiologic levels of MCP-1 are important for the ovulatory process [20]. As far as aging and obesity, data have shown that systemic MCP-1 levels are increased in aging animals [14][15][16] and that there is an increased abundance of this pro-inflammatory chemokine in both white adipose tissue and plasma in obese animals [17][18][19]. Additionally, we have recently demonstrated in women undergoing in vitro fertilization (IVF) that serum and follicular fluid MCP-1 are positively correlated with body mass index (BMI) (Obehi et al, manuscript under review).…”