Agarose Surface Coating Influences Intracellular Accumulation and Enhances Payload Stability of a Nano-delivery System

Rosa, Enrica De; Chiappini, Ciro; Fan, Dong; Liu, Xuewu; Ferrari, Mauro; Tasciotti, Ennio

doi:10.1007/s11095-011-0453-2

Cited by 29 publications

(32 citation statements)

References 43 publications

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“…The ζ potential of the silicon particles, prior to encapsulation in the PLGA shell, was analyzed using a Zetasizer nano ZS (Malvern Instruments Ltd., Southborough, MA, USA), as previously described. 26 APTES-modified MSV and the resulting PLGA-MSV microspheres were analyzed by Fourier transformed infrared spectroscopy (FTIR). Briefly, a KBr pellet was made by mixing KBr to freeze-dried PLGA-MSV.…”

Section: Silicon Particles Fabrication and Surface Modificationmentioning

confidence: 99%

“…24,25 Recently, our group proposed a composite carrier consisting of the nanostructured silicon multistage vector (MSV) 19 and a PLGA outer shell (PLGA-MSV), which, being of great interest in the field of tissue engineering, was demonstrated to allow for the release of high amounts of proteins, while ensuring their stability. 26,27 The aim of this study was to optimize a translational approach for fine temporal and spatial control over the release of proteins, in vivo. Hence, we proposed newly developed multiscale composite microspheres based on a core consisting of the nanostructured silicon MSV and a PLGA outer shell.…”

Section: Introductionmentioning

confidence: 99%

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PLGA-Mesoporous Silicon Microspheres for the in Vivo Controlled Temporospatial Delivery of Proteins

Minardi

Pandolfi

Taraballi

et al. 2015

ACS Appl. Mater. Interfaces

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In regenerative medicine, the temporospatially controlled delivery of growth factors (GFs) is crucial to trigger the desired healing mechanisms in the target tissues. The uncontrolled release of GFs has been demonstrated to cause severe side effects in the surrounding tissues. The aim of this study was to optimize a translational approach for the fine temporal and spatial control over the release of proteins, in vivo. Hence, we proposed a newly developed multiscale composite microsphere based on a core consisting of the nanostructured silicon multistage vector (MSV) and a poly(dl-lactide-co-glycolide) acid (PLGA) outer shell. Both of the two components of the resulting composite microspheres (PLGA-MSV) can be independently tailored to achieve multiple release kinetics contributing to the control of the release profile of a reporter protein in vitro. The influence of MSV shape (hemispherical or discoidal) and size (1, 3, or 7 μm) on PLGA-MSV's morphology and size distribution was investigated. Second, the copolymer ratio of the PLGA used to fabricate the outer shell of PLGA-MSV was varied. The composites were fully characterized by optical microscopy, scanning electron microscopy, ζ potential, Fourier transform infrared spectroscopy, and thermogravimetric analysis-differential scanning calorimetry, and their release kinetics over 30 days. PLGA-MSV's biocompatibility was assessed in vitro with J774 macrophages. Finally, the formulation of PLGA-MSV was selected, which concurrently provided the most consistent microsphere size and allowed for a zero-order release kinetic. The selected PLGA-MSVs were injected in a subcutaneous model in mice, and the in vivo release of the reporter protein was followed over 2 weeks by intravital microscopy, to assess if the zero-order release was preserved. PLGA-MSV was able to retain the payload over 2 weeks, avoiding the initial burst release typical of most drug delivery systems. Finally, histological evaluation assessed the biocompatibility of the platform in vivo.

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Section: Silicon Particles Fabrication and Surface Modificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PLGA-Mesoporous Silicon Microspheres for the in Vivo Controlled Temporospatial Delivery of Proteins

Minardi

Pandolfi

Taraballi

et al. 2015

ACS Appl. Mater. Interfaces

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“…In addition, PSi has potential for cancer chemotherapy applications, for example oxidation triggered delivery of doxorubicin has been investigated in vitro (10). Also proteins or peptides, such as human/bovine serum albumin (HSA/BSA), papain and insulin, have been loaded on PSi surfaces with different surface modifications and investigated in vitro, including intracellular protein delivery (6)(7)(8)11,12). Our recent in vivo studies with several different physiological parameters indicated that THCPSi microparticles could sustain the in vivo effects of peptides (13,14).…”

Section: Introductionmentioning

confidence: 99%

Mesoporous Silicon (PSi) for Sustained Peptide Delivery: Effect of PSi Microparticle Surface Chemistry on Peptide YY3-36 Release

et al. 2011

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“…In addition, the release of payloads from pSi can be modulated using a 'capping' or 'gate-keeping' approach. For example, pSi can be capped with poly(l-lactide) [58], poly(dl-lactide-co-glycolide) [59], agarose [60], cellular membranes [61] and hydrogels [62] allowing for the release of proteins and chemotherapeutics or act as sensors. Furthermore, these approaches enable a responsive drug delivery platform capable of delivering payloads in response to variations in temperature [63], voltage [64], proteases [65] and pH [66].…”

Section: Loading and Release Of Payloads From Psimentioning

confidence: 99%

Physicochemical Properties Affect the Synthesis, Controlled Delivery, Degradation and Pharmacokinetics of Inorganic Nanoporous Materials

Yazdi

Žiemys

Evangelopoulos

et al. 2015

Nanomedicine (Lond.)

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Controlling size, shape and uniformity of porous constructs remains a major focus of the development of porous materials. Over the past two decades, we have seen significant developments in the fabrication of new, porous-ordered structures using a wide range of materials, resulting in properties well beyond their traditional use. Porous materials have been considered appealing, due to attractive properties such as pore size length, morphology and surface chemistry. Furthermore, their utilization within the life sciences and medicine has resulted in significant developments in pharmaceutics and medical diagnosis. This article focuses on various classes of porous materials, providing an overview of principle concepts with regard to design and fabrication, surface chemistry and loading and release kinetics. Furthermore, predictions from a multiscale mathematical model revealed the role pore length and diameter could have on payload release kinetics.

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Agarose Surface Coating Influences Intracellular Accumulation and Enhances Payload Stability of a Nano-delivery System

Cited by 29 publications

References 43 publications

PLGA-Mesoporous Silicon Microspheres for the in Vivo Controlled Temporospatial Delivery of Proteins

PLGA-Mesoporous Silicon Microspheres for the in Vivo Controlled Temporospatial Delivery of Proteins

Mesoporous Silicon (PSi) for Sustained Peptide Delivery: Effect of PSi Microparticle Surface Chemistry on Peptide YY3-36 Release

Physicochemical Properties Affect the Synthesis, Controlled Delivery, Degradation and Pharmacokinetics of Inorganic Nanoporous Materials

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