Agarose-based freeze-dried capsules prepared by the oil-induced biphasic hydrogel particle formation approach for the protection of sensitive probiotic bacteria

Alehosseini, Ali; Pulgar, Eva-María Gomez del; Fabra, María José; Gómez‐Mascaraque, Laura G.; Benı́tez-Páez, Alfonso; Sarabi-Jamab, Mahboobe; Ghorani, Behrouz; López‐Rubio, Amparo

doi:10.1016/j.foodhyd.2018.08.032

Cited by 58 publications

(12 citation statements)

References 45 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluorescence‐stained dead cells appear red whereas live cells appear green, so that the ratio of live‐to‐dead cells can be detected by confocal microscopy or flow cytometry. Encapsulated probiotics have also been labeled using a 1/10 SYBR® Green I Nucleic Acid stain (Lonza), which enabled the determination of cell distribution inside microgels (Alehosseini et al., ). Probiotics can also be detected by incorporating a luminescence‐emitting plasmid ( p GEN‐ lux CDABE), which allows live probiotics to be detected using an in vivo imaging system (IVIS) (Anselmo et al., ).…”

Section: Emerging Methods For Evaluating Probiotic Delivery System Efmentioning

confidence: 99%

Progress in microencapsulation of probiotics: A review

Yao

Xie

et al. 2020

Comp Rev Food Sci Food Safe

258

142

View full text Add to dashboard Cite

The potential health benefits of probiotics may not be realized because of the substantial reduction in their viability during food storage and gastrointestinal transit. Microencapsulation can be used to enhance the resistance of probiotics to unfavorable conditions. A range of oral delivery systems has been developed to increase the level of probiotics reaching the colon including embedding and coating systems. This review introduces emerging strategies for the microencapsulation of probiotics and highlights the key mechanisms of their stress–tolerance properties. Recent in vitro and in vivo models for evaluation of the efficiency of probiotic delivery systems are also reviewed. Encapsulation technologies are required to maintain the viability of probiotics during storage and within the human gut so as to increase their ability to colonize the colon. These technologies work by protecting the probiotics from harsh environmental conditions, as well as increasing their mucoadhesive properties. Typically, the probiotics are either embedded inside or coated with food‐grade materials such as biopolymers or lipids. In some cases, additional components may be coencapsulated to enhance their viability such as nutrients or protective agents. The importance of having suitable in vitro and in vivo models to evaluate the efficiency of probiotic delivery systems is also emphasized.

show abstract

Section: Emerging Methods For Evaluating Probiotic Delivery System Efmentioning

confidence: 99%

Progress in microencapsulation of probiotics: A review

Yao

Xie

et al. 2020

Comp Rev Food Sci Food Safe

258

142

View full text Add to dashboard Cite

show abstract

“…Alehosseini et al, in 2019 [101] reported a simple and fast approach with potential for probiotic protection, which consisted on the formation of agarose-based hydrogel. They used the oilinduced biphasic hydrogel particle formation to encapsulate the sensitive strain Bifidobacterium pseudocatenulatum CECT 7765: this system guaranteed the formation of a protective matrix for the bacterium.…”

Section: Bacteriamentioning

confidence: 99%

Progress in hydrogels for sensing applications: a review

Pinelli

Magagnin

Rossi

2020

Materials Today Chemistry

View full text Add to dashboard Cite

There are several developments taking place in the field of sensors driven by the world today requirements. One of the most important novelties of the last two decades in the field is represented by the hydrogel-based sensors which constitute a wide family of innovative smart sensing devices relevant for many different applications. Hydrogels in fact are hydrophilic, biocompatible and highly water swellable polymer networks able to convert chemical energy into mechanical energy, with the great peculiarity to be able to respond to external stimuli. These characteristics have ensured them considerable recognition as valuable tool for smart sensing and diagnostics. The aim of this review is to focus on the advances obtained in the field in the last ten years.

show abstract

“…This method proves to be useful for protecting oxygen-sensitive microorganisms as the capsules were prepared in lipid medium, thereby restricting the interference by oxygen. The probiotic cells were even safeguarded in the acidic conditions of the stomach and an improvement in viability was observed when compared to uncoated probiotic strains [40].…”

Section: Freeze Dryingmentioning

confidence: 99%

Probiotics for treating disorders: microencapsulation a boon to potentiate their therapeutic applications

2020

Biointerface Res Appl Chem

View full text Add to dashboard Cite

Probiotics are nutraceutical products which have been used substantially for supplementation of the intestinal microbiota. Probiotics have been indicated in the therapy of various disorders such as gastric ulcer, diarrhoea, gastroenteritis, inflammatory bowel disease, colon cancer, urogenital infection, allergy, respiratory infection and to promote the individual’s well-being. The present review article focuses on the mechanism followed by probiotics for inactivating the harmful antigens. The various microencapsulation techniques useful in improving the processing, transit and storage stability of probiotics have also been described. Microencapsulation process linked protection and increased survival rate of probiotics have been discussed systematically.

show abstract

Agarose-based freeze-dried capsules prepared by the oil-induced biphasic hydrogel particle formation approach for the protection of sensitive probiotic bacteria

Cited by 58 publications

References 45 publications

Progress in microencapsulation of probiotics: A review

Progress in microencapsulation of probiotics: A review

Progress in hydrogels for sensing applications: a review

Probiotics for treating disorders: microencapsulation a boon to potentiate their therapeutic applications

Contact Info

Product

Resources

About