Agarose-based biomaterials for advanced drug delivery

Yazdi, Mohsen Khodadadi; Taghizadeh, Ali; Taghizadeh, Mohsen; Stadler, Florian J.; Farokhi, Mehdi; Mottaghitalab, Fatemeh; Zarrintaj, Payam; Ramsey, Joshua D.; Seidi, Farzad; Saeb, Mohammad Reza; Mozafari, Masoud

doi:10.1016/j.jconrel.2020.07.028

Cited by 143 publications

(57 citation statements)

References 153 publications

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“…Agarose displays several advantages such as its low cost, high availability, and non-cytotoxicity ( Das and Pal, 2015 ). It is recognized that by controlling the agarose hydrogel porosity ( Marras-Marquez et al., 2014 ; Khodadadi Yazdi et al., 2020 ), via its degree of crosslinking, the drug release can be modulated. Therefore, in order to have a better control on the antibiotic release kinetic and hydrogel cohesion and based on literature data, the effect of TA and CaCl 2 , were evaluated.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, agarose hydrogels ensure cell adhesion and provide adequate oxygen and nutrient permeability for cell growth ( Zarrintaj et al., 2018 ). Besides, agarose is widely used as component in controlled drug delivery system ( Meilander et al., 2003 ; Marras-Marquez et al., 2014 ; Nazemi et al., 2014 ; Awadhiya et al., 2016 ; Hu et al., 2016 ; Zarrintaj et al., 2018 ; Khodadadi Yazdi et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin

Soylu

Chevallier

Copes

et al. 2021

Front. Cell. Infect. Microbiol.

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IntroductionThe use of spinal implants for the treatment of back disorders is largely affected by the insurgence of infections at the implantation site. Antibacterial coatings have been proposed as a viable solution to limit such infections. However, despite being effective at short-term, conventional coatings lack the ability to prevent infections at medium and long-term. Hydrogel-based drug delivery systems may represent a solution controlling the release of the loaded antibacterial agents while improving cell integration. Agarose, in particular, is a biocompatible natural polysaccharide known to improve cell growth and already used in drug delivery system formulations. In this study, an agarose hydrogel-based coating has been developed for the controlled release of gentamicin (GS).MethodsSand blasted Ti6Al4V discs were grafted with dopamine (DOPA) solution. After, GS loaded agarose hydrogels have been produced and additioned with tannic acid (TA) and calcium chloride (CaCl2) as crosslinkers. The different GS-loaded hydrogel formulations were deposited on Ti6Al4V-DOPA surfaces, and allowed to react under UV irradiation. Surface topography, wettability and composition have been analyzed with profilometry, static contact angle measurement, XPS and FTIR spectroscopy analyses. GS release was performed under pseudo-physiological conditions up to 28 days and the released GS was quantified using a specific ELISA test. The cytotoxicity of the produced coatings against human cells have been tested, along with their antibacterial activity against S. aureus bacteria.ResultsA homogeneous coating was obtained with all the hydrogel formulations. Moreover, the coatings presented a hydrophilic behavior and micro-scale surface roughness. The addition of TA in the hydrogel formulations showed an increase in the release time compared to the normal GS-agarose hydrogels. Moreover, the GS released from these gels was able to significantly inhibit S. aureus growth compared to the GS-agarose hydrogels. The addition of CaCl2 to the gel formulation was able to significantly decrease cytotoxicity of the TA-modified hydrogels.ConclusionsDue to their surface properties, low cytotoxicity and high antibacterial effects, the hereby proposed gentamicin-loaded agarose-hydrogels provide new insight, and represent a promising approach for the surface modification of spinal implants, greatly impacting their application in the orthopedic surgical scenario.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin

Soylu

Chevallier

Copes

et al. 2021

Front. Cell. Infect. Microbiol.

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“…As shown in Figure 8 a, the CPFX-loaded agarose hydrogel (AG10) released the drug after 9 h at a similar rate, 45% and 53%, at pH 1.2 and pH 7.4, respectively. This is because the natural agarose hydrogel would control the diffusion phenomena that occurred after the initial burst release [ 8 ]. However, the agarose/succinoglycan (AG4/SG6) hydrogels with CPFX showed a significant difference between pH 1.2 and pH 7.4, where all of the CPFX was released within 33 h at pH 7.4.…”

Section: Resultsmentioning

confidence: 99%

“…This water-soluble polysaccharide generally forms a network structure at low temperatures, creating a water-insoluble special gel-type structure [ 5 , 6 ]. Agarose hydrogels have attracted some attention for tissue engineering [ 7 ], drug delivery [ 8 ], and rapid wound healing [ 9 ]. This is due to their good biocompatibility, thermo-reversibility, stability in vivo systems, and high mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Flexible pH-Responsive Agarose/Succinoglycan Hydrogels for Controlled Drug Release

Kim

Hong

et al. 2021

Polymers

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Agarose/succinoglycan hydrogels were prepared as pH-responsive drug delivery systems with significantly improved flexibility, thermostability, and porosity compared to agarose gels alone. Agarose/succinoglycan hydrogels were made using agarose and succinoglycan, a polysaccharide directly isolated from Sinorhizobium meliloti. Mechanical and physical properties of agarose/succinoglycan hydrogels were investigated using various instrumental methods such as rheological measurements, attenuated total reflection–Fourier transform infrared (ATR-FTIR) spectroscopic analysis, X-ray diffraction (XRD), and field-emission scanning electron microscopy (FE-SEM). The results showed that the agarose/succinoglycan hydrogels became flexible and stable network gels with an improved swelling pattern in basic solution compared to the hard and brittle agarose gel alone. In addition, these hydrogels showed a pH-responsive delivery of ciprofloxacin (CPFX), with a cumulative release of ~41% within 35 h at pH 1.2 and complete release at pH 7.4. Agarose/succinoglycan hydrogels also proved to be non-toxic as a result of the cell cytotoxicity test, suggesting that these hydrogels would be a potential natural biomaterial for biomedical applications such as various drug delivery system and cell culture scaffolds.

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“…These directions are based on tunable features of agarose, which can result in adjustable characteristics similar to native tissues [225]. In addition, applications of this biomaterial for targeted drug delivery have been recently discussed in the review entitled "Agarose-based biomaterials for advanced drug delivery" [227]. Finally, agarose gels can be used in 3D bioprinting [228].…”

Section: Patentsmentioning

confidence: 99%

Progress in Modern Marine Biomaterials Research

et al. 2020

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The growing demand for new, sophisticated, multifunctional materials has brought natural structural composites into focus, since they underwent a substantial optimization during long evolutionary selection pressure and adaptation processes. Marine biological materials are the most important sources of both inspiration for biomimetics and of raw materials for practical applications in technology and biomedicine. The use of marine natural products as multifunctional biomaterials is currently undergoing a renaissance in the modern materials science. The diversity of marine biomaterials, their forms and fields of application are highlighted in this review. We will discuss the challenges, solutions, and future directions of modern marine biomaterialogy using a thorough analysis of scientific sources over the past ten years.

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Agarose-based biomaterials for advanced drug delivery

Cited by 143 publications

References 153 publications

A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin

A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin

Fabrication of Flexible pH-Responsive Agarose/Succinoglycan Hydrogels for Controlled Drug Release

Progress in Modern Marine Biomaterials Research

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