Agar Gel Electrophoresis for the Determination of Isozymes of Lactic and Malic Dehydrogenase

Yakulis, Vincent; Gibson, Coultas W.; Heller, Paul

doi:10.1093/ajcp/38.4.378

Cited by 42 publications

(12 citation statements)

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“…MDH isozyme patterns Yakulis et al (1962) found the MDH isozyme patterns to be concentrated in two bands in all human tissues except erythrocytes . The cell-sap enzyme (MDHs) migrates towards the anode when subjected to electrophoresis in Trig-HCl buffer , pH 8.4, whereas the mitochondrial enzyme (MDHm) towards the cathode.…”

Section: Cpk Isozyme Patternsmentioning

confidence: 98%

Creatine Phosphokinase and Malate Dehydrogenase Isozymes in Heart Muscle of Sudden Death Cases

Suzuki

Kashimura

Umetsu

1980

Tohoku J. Exp. Med.

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-CPK and MDK isozyme patterns of the heart muscle in cases of sudden death were studied to investigate the relationship between their changes and the causes of death . In most of death cases from violence, asphyxia, natural disease and poisoning, the percentage of CPK-MB was about 23-34% of the total CPK, and that of MDHm was about 35-50% of the total MDH. In a few death cases of coronary sclerosis and hypertrophy of the heart, the percentages of CPK-MB and MDHm decreased. The percentages of CPK-MB and MDHm in neonates were less than those in adults and they were about 7-15% and 25-34% of the total CPK and MDH, respectively. In more than half of the cases of both cardiac death from unknown cause and cot death, the average percentages of CPK-MB and MDHm decreased significantly.

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Section: Cpk Isozyme Patternsmentioning

confidence: 98%

Creatine Phosphokinase and Malate Dehydrogenase Isozymes in Heart Muscle of Sudden Death Cases

Suzuki

Kashimura

Umetsu

1980

Tohoku J. Exp. Med.

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“…The gels were also stained for caeruloplasmin ussng p-phenylenediamine in acetate buffer (Uriel, 1958) and for the isoenzymes of lactate dehydrogenase (Yakulis, Gibson, and Heller, 1962).…”

Section: Methodsmentioning

confidence: 99%

Polyacrylamide disc electrophoresis of the proteins of cerebrospinal fluid and brain

Monseu¹,

Cumings²

1965

Journal of Neurology, Neurosurgery & Psychiatry

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Fox, Thurman, and Boulter, 1963).The gels were stained with 1 % naphthalene black in 7 % acetic acid, and the excess stain removed by frequent washings in 7% acetic acid, or electrophoretically, both methods giving identical results.Reproducibility was tested by putting up the same cerebrospinal fluid in many tubes at both the same concentration as well as at varying concentrations of protein content.

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“…In the heart muscle of the experimental group, subunit peptide M tended to increase, and CPK-MM was about 80-98% of the total CPK (Table 4). MDII of the heart muscle consists of two different isozymes, that is, the cell-sap enzyme (MDHs) and mitochondrial enzyme (MDHm) (Yakulis et al 1962). In the heart muscle of the control group, MDHm was about 44-59% and MDHs was about 42-54%.…”

Section: Electrophysiological and Morphological Observationsmentioning

confidence: 99%

Experimental model of sudden cardiac death from unexplained cause.

Suzuki

Kashimura

Umetsu

1982

Tohoku J. Exp. Med.

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Agar Gel Electrophoresis for the Determination of Isozymes of Lactic and Malic Dehydrogenase

Cited by 42 publications

References 0 publications

Creatine Phosphokinase and Malate Dehydrogenase Isozymes in Heart Muscle of Sudden Death Cases

Creatine Phosphokinase and Malate Dehydrogenase Isozymes in Heart Muscle of Sudden Death Cases

Polyacrylamide disc electrophoresis of the proteins of cerebrospinal fluid and brain

Experimental model of sudden cardiac death from unexplained cause.

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