Agar/galactomannan blends for strawberry (Fragaria x ananassa Duchesne) cv. Pelican micropropagation

Lucyszyn, Neoli; Quoirin, Marguerite; Koehler, Henrique Soares; Reicher, Fany; Sierakowski, Maria Rita

doi:10.1016/j.scienta.2005.11.005

Cited by 16 publications

(21 citation statements)

References 19 publications

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“…The difference arises from the stronger interaction that the sulfate and pyruvate groups of agar form with water molecules, resulting in higher viscosity and consistency of the media, compared to the interaction between the hydroxyl moieties of starch and water molecules. A decrease in media consistency and solidification was also reported by Jain and Babbar (2011) and by Lucyszyn et al (2006) in the partial substitution of agar with guar gum, isubgol or xanthan, and with galactomannans, respectively. However, that lowers viscosity of the culture media showed positive effects on the plantlets growth.…”

Section: Discussionsupporting

confidence: 73%

Efficiency of the partial substitution of agar with potato starch on the growth and phytochemical parameters of lulo (Solanum quitoense) cultured in vitro

D.¹,

Oswaldo²,

Jose³

et al. 2017

Afr. J. Biotechnol.

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The demand for agar, thanks to its different uses, generates the need to look for alternative substances that replace it. This work studied the influence of four different additions of potato starch into agar media (T1 = 50%, T2 = 45%, T3 = 40% and T4 = 0%) on the growth and chemical composition of essential oils of lulo (Solanum quitoense) explants cultured in vitro. The lengths, fresh and dry biomass weights, and number of nodes of the lulo explants were selected as growth indices and compared against either viscosity of the modified media or extraction percentage and relative concentration of the main components in the essential oils. A higher statistic variability and amount, of the lengths, fresh biomass and number of nodes, were found for the explants cultured in modified agar media compared to those cultured in pure agar. In contrast, minimal variation in metabolite extractions and concentrations were obtained as a function of potato starch addition. Cyclotetradecane, neophytadiene, 1-hexadecene, phytol, oleamide and 3-octadecene were found as the main components of the essential oils by gas chromatography-mass spectrometry (GC-MS). It was found that T1 was the treatment that yielded the best results in the analyzed variables.

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Section: Discussionsupporting

confidence: 73%

Efficiency of the partial substitution of agar with potato starch on the growth and phytochemical parameters of lulo (Solanum quitoense) cultured in vitro

D.¹,

Oswaldo²,

Jose³

et al. 2017

Afr. J. Biotechnol.

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“…An interaction between the cytokinin content and the gelling agents used was also observed in other experiments with blackberry (Fira et al 2010) and Aloe polyphylla (Ivanova and van Staden 2011). A stimulating effect of galactomannan extracted from guar gum was also proven in strawberry (Lucyszyn et al 2006a) and in Nicotiana tabacum (Lucyszyn et al 2007). In these experiments, the blends improved the quality of the shoots, including the rooting parameters, for both species and leaf parameters in Nicotiana.…”

Section: Introductionsupporting

confidence: 70%

Comparison of the rheological and diffusion properties of some gelling agents and blends and their effects on shoot multiplication

Dobránszki

Magyar-Tábori

Tombácz

2011

Plant Biotechnol Rep

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The rheological and diffusion properties of blends of agar/guar gum, agar/Phytagel and Phytagel/guar gum were analysed and compared to those properties of agar or Phytagel applied alone at two different gelling concentrations. Moreover, their effects on the shoot multiplication of the apple scion Galaxy and two black locust clones (SF63, SF82) were studied, and their cost benefits over agar were calculated. Elastic hydrogel formation was demonstrated for each blend by rheological measurements, but the gel strength depended on the types and concentrations of the applied gelling agents and blends. Guar gum was able to speed the diffusion in the different blends, and diffusion was independent of gel strength. The rate of shoot multiplication increased (to 8.9 shoots per explant) and the percent of hyperhydrated shoots decreased (to 12%) when the blend of agar/guar gum was used for the shoot multiplication of apple. Similarly, the highest multiplication rates of black locust clones (between 3.9 and 4.1) were obtained on media solidified by blends containing guar gum. The best shoot performance with the lowest percent of hyperhydrated shoots (11-12% in SF63 and 2-23% in SF82) was achieved using agar alone or the agar/guar gum blend. The shoot multiplication was improved of both species and the production cost was reduced by 42% by using the agar/guar gum blend.

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“…First, the ground seeds were submitted to an extraction of pigments and lipids with ethyl ether under reflux in a Soxhlet system. The isolation of the galactomannan (GM) was done as described by Lucyszyn et al [9] The GM had a Manannose:Galactose (Man:-Gal) ratio of 2.1, determined by alditol acetate composition [10] and confirmed by 1 H and 13 C NMR, in D 2 O at 75 8C, from the integrals of the anomeric regions (data not shown).…”

Section: Experimental Part Materialsmentioning

confidence: 99%

Oxidation and N‐Alkylation at the C‐6 Position of Galactomannan Extracted from Caesalpinia ferrea var. ferrea Seeds

Souza

Lucyszyn

Ferraz

et al. 2011

Macromolecular Symposia

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Summary: Carboxyl (GMOX) and carboxyl-aminoalkylated derivatives (GMOXD) of galactomannan (GM), extracted from the seeds of Caesalpinia ferrea (Pau-ferro; Brazilian Ironwood), were synthesised by selective modification of the polysaccharide at the C-6 position in the presence of TEMPO reagent (2,2,6,6-tetramethylpiperidine-1-oxyl) and dodecylamine, with high yields (77 and 94% for GMOX and GMOXD, respectively). The chemical modifications were confirmed by Fourier-transform infrared (FTIR) and nuclear magnetic resonance ( 13 C-NMR) spectroscopy. Analysis by gel permeation chromatography (GPC) coupled to refractive index (RI), lightscattering (LS) and viscometer detectors revealed a decrease in molar-mass values and the intrinsic viscosity and an increase in polydispersity. Modification of galactomannan with a primary amine led to an interesting anionic water-soluble polymeric surfactant, as determined by interfacial tension values at 24 o C. The critical aggregation and micellar concentration values of amphiphilic GMOXD were determined to be 0.48 and 0.89% (w/v), respectively. The development of polyelectrolyte and amphiphilic derivatives of GM and their physicochemical characterisation can contribute to numerous product applications such as nano-structured films, cosmetics and pharmaceuticals.

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Agar/galactomannan blends for strawberry (Fragaria x ananassa Duchesne) cv. Pelican micropropagation

Cited by 16 publications

References 19 publications

Efficiency of the partial substitution of agar with potato starch on the growth and phytochemical parameters of lulo (Solanum quitoense) cultured in vitro

Efficiency of the partial substitution of agar with potato starch on the growth and phytochemical parameters of lulo (Solanum quitoense) cultured in vitro

Comparison of the rheological and diffusion properties of some gelling agents and blends and their effects on shoot multiplication

Oxidation and N‐Alkylation at the C‐6 Position of Galactomannan Extracted from Caesalpinia ferrea var. ferrea Seeds

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