African horse sickness virus serotype 4 antigens, VP1-1, VP2-2, VP4, VP7 and NS3, induce cytotoxic T cell responses in vitro

Faber, Erika; Kleef, Mirinda Van; Tshilwane, Selaelo Ivy; Pretorius, Ashley

doi:10.1016/j.virusres.2016.04.007

Cited by 13 publications

(12 citation statements)

References 49 publications

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“…Furthermore, AHSV VP7 purified from BHK-infected cells was able to protect BALB/c mice against lethal challenge, although it induced low antibody titres as detected with ELISA, suggesting that such protection could conceivably also involve cell-mediated responses (Wade-Evans et al 1997 ). This suggestion was supported recently where VP7 induced equine cytotoxic T-cell responses in vivo (Faber et al 2016 ). One region was mapped at the centre of NS2 (27 aa).…”

Section: Discussionsupporting

confidence: 69%

“…It needs to be confirmed whether these regions are in themselves involved in immunological protection. Once confirmed as being potentially protective B-cell epitopes, they could conceivably be combined with suitable T-cell epitopes (Faber et al 2016 ) to form a vaccine construct capable of evoking both humoral and cellular immunity. Apart from vaccine development, B-cell epitopes on NS2, VP6, NS3 and VP7 may also assist in the development of diagnostic tests.…”

Section: Discussionmentioning

confidence: 99%

“…This library was constructed essentially as previously described (Du Plessis & Jordaan 1996 ; Fehrsen et al 2005 ; Gupta et al 1999 ) by using existing cDNA copies of each AHSV-4 genome segment cloned into either pET102D-TOPO or pGEMT (Faber et al 2016 ). Large segments encoding VP1, VP2 and VP3 were cloned as two fragments.…”

Section: Methodsmentioning

confidence: 99%

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B-cell epitopes of African horse sickness virus serotype 4 recognised by immune horse sera

Mathebula

Faber

Wyngaardt

et al. 2017

Onderstepoort j. vet. res.

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Identifying antigenic proteins and mapping their epitopes is important for the development of diagnostic reagents and recombinant vaccines. B-cell epitopes of African horse sickness virus (AHSV) have previously been mapped on VP2, VP5, VP7 and NS1, using mouse, rabbit and chicken monoclonal antibodies. A comprehensive study of the humoral immune response of five vaccinated horses to AHSV-4 antigenic peptides was undertaken. A fragmented-genome phage display library expressing a repertoire of AHSV-4 peptides spanning the entire genome was constructed. The library was affinity selected for binders on immobilised polyclonal immunoglobulin G (IgG) isolated from horse sera collected pre- and post-immunisation with an attenuated AHSV-4 monovalent vaccine. The DNA inserts of binding phages were sequenced with Illumina high-throughput sequencing. The data were normalised using pre-immune IgG-selected sequences. More sequences mapped to the genes coding for NS3, VP6 and VP5 than to the other genes. However, VP2 and VP5 each had more antigenic regions than each of the other proteins. This study identified a number of epitopes to which the horse’s humoral immune system responds during immunisation with AHSV-4.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

B-cell epitopes of African horse sickness virus serotype 4 recognised by immune horse sera

Mathebula

Faber

Wyngaardt

et al. 2017

Onderstepoort j. vet. res.

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“…The FATT-CTL assay was carried out as described previously, with minor modifications [21,22]. Firstly, the method was optimised for use with sheep PBMC.…”

Section: Fatt-ctl Assaymentioning

confidence: 99%

“…To test if any of the selected antigens, previously tested in animals, induce CD8 + T cells to kill target cells, a human PBMC based FATT-CTL assay was adapted for use with sheep PBMC [21]. This assay was recently optimized for use with horse PBMC [22], and this study is the between the animals, they appear to be less MHC restricted than the proteins. It would have been very interesting to determine the MHC I alleles for these animals.…”

Section: Peptides That Stimulate Cd8 + Cd45ro + and Cd8 + Ifn-γ + T Cmentioning

confidence: 99%

Ehrlichia ruminantium antigens and peptides induce cytotoxic T cell responses in vitro

Thema

Tshilwane

Sơn

et al. 2019

Veterinary Immunology and Immunopathology

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Since CD8 + T cells play an important role in resistance to infection with heartwater, effective vaccines against this disease will likely require identification of antigens that contain CD8 + T cell epitopes responsible for cytotoxic T lymphocyte (CTL) responses. With the use of the fluorescent antigen-transfected target cell (FATT)-CTL assay, IFN-γ ELISPOT and flow cytometry, peptides that induce CTL, proliferation of CD8+T cells and IFN-γ production were identified as possible target antigens for vaccine development. Of particular relevance was the finding that different peptides from different antigens were able to elicit varied cytotoxic activities by immune peripheral blood mononuclear cells (PBMC) from heartwater immune tick-infected sheep. Several peptides derived from Erum0660, Erum2330, Erum2540, Erum2580 and Erum5000 induced CTL in immune sheep PBMC. Peptide Erum2540-6 was the only peptide that induced significant CTL, CD8 + CD45RO + and CD8 + IFN-γ + by PBMC from all three sheep, and Erum2540 and p2540-20 induced the highest % CTL response in all three outbred sheep. These results suggest that these epitopes may be of major importance in heartwater recombinant vaccine development.

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Virulent African horse sickness virus serotype 4 interferes with the innate immune response in horse peripheral blood mononuclear cells in vitro

Faber

Tshilwane

Kleef

et al. 2021

Infection, Genetics and Evolution

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African horse sickness virus serotype 4 antigens, VP1-1, VP2-2, VP4, VP7 and NS3, induce cytotoxic T cell responses in vitro

Cited by 13 publications

References 49 publications

B-cell epitopes of African horse sickness virus serotype 4 recognised by immune horse sera

B-cell epitopes of African horse sickness virus serotype 4 recognised by immune horse sera

Ehrlichia ruminantium antigens and peptides induce cytotoxic T cell responses in vitro

Virulent African horse sickness virus serotype 4 interferes with the innate immune response in horse peripheral blood mononuclear cells in vitro

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