AFM Studies of Cellular Mechanics during Osteogenic Differentiation of Human Amniotic Fluid-derived Stem Cells

Chen, Qian; Xiao, Pan; Chen, Jianan; Cai, Jiye; Cai, Xuan; Ding, Hui; Pan, Yunlong

doi:10.2116/analsci.26.1033

Cited by 28 publications

(28 citation statements)

References 25 publications

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“…It has been reported that the roughness of amniotic fluid-derived stem cells increases during osteogenic differentiation because of mineralization of the differentiated osteoblasts after an induction culture of 4 weeks. 27 However, according to our results, there was no significant difference of the surface roughness between MSC and NHOst cells, which may be due to the short cell culture time.…”

Section: Resultsmentioning

confidence: 44%

Morphological and Mechanical Properties of Osteosarcoma Microenvironment Cells Explored by Atomic Force Microscopy

Wang

Yang

et al. 2016

ANAL. SCI.

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Cell mechanical properties that depend on cytoskeleton architecture are critical to the mechanotransduction process, and have great potential for cancer diagnosis and therapy. In this study, the morphological and mechanical properties of typical osteosarcoma microenvironment cells, including mesenchymal stem cells (MSC), normal human osteoblast cells (NHOst) and osteosarcoma cells (MG-63), were compared using atomic force microscopy (AFM). The MG-63 cells were smaller and thicker than the MSC and NHOst cells. The membrane roughness of MG-63 cells was higher than that of MSC and NHOst cells. The MG-63 cells had lower stiffness than their normal counterparts due to their reduced organization of the cytoskeleton structure. The cell stiffness influenced the mechanotransduction. The MG-63 cells had a lower percentage of nuclear YAP/TAZ compared with the MSC and NHOst cells. The F-actin assembly was disrupted by the cytochalasin D (cyto D) treatment used to investigate its influence on mechanotransduction. Disruption of the cytoskeleton leaded to a decrease of the cell stiffness, and reduced the nuclear YAP/TAZ percentage, indicating its inhibition in the cell mechanotransduction process. This study would shed light on the development of a novel cancer diagnosis strategy and would contribute to reveal the relationship between the cytoskeleton structure and the cell mechanical properties.

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Section: Resultsmentioning

confidence: 44%

Morphological and Mechanical Properties of Osteosarcoma Microenvironment Cells Explored by Atomic Force Microscopy

Wang

Yang

et al. 2016

ANAL. SCI.

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“…Similar to the responsiveness to mechanical loads, cytoskeletal adaptations that occur during osteogenic commitment of stem cells involve the reorganization of stress fibers and the increase of membrane roughness, which conspire towards increased cellular stiffness (Chen et al, 2010). Cellular morphology and membrane roughness of bone-inducing cells were shown to be associated with the presence of mechanical loads, as the removal of gravitational loads with high magnitude of environmental gradient (0 g using magnetic levitation) reduced the height and the roughness of these cells (Qian et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Bone marrow stem cells adapt to low-magnitude vibrations by altering theircytoskeleton during quiescence and osteogenesis

Demiray¹,

Özçivici

2015

Turk J Biol

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Application of mechanical vibrations is anabolic to bone tissue, not only by guiding mature bone cells to increased formation, but also by increasing the osteogenic commitment of progenitor cells. However, the sensitivity and adaptive response of bone marrow stem cells to this loading regimen has not yet been identified. In this study, we subjected mouse bone marrow stem cell line D1-ORL-UVA to daily mechanical vibrations (0.15 g, 90 Hz, 15 min/day) for 7 days, both during quiescence and osteogenic commitment, to identify corresponding ultrastructural adaptations on cellular and molecular levels. During quiescence, mechanical vibrations significantly increased total actin content and actin fiber thickness, as measured by phalloidin staining and fluorescent microscopy. Cellular height also increased, as measured by atomic force microscopy, along with the expression of focal adhesion kinase (PTK2) mRNA levels. During osteogenesis, mechanical vibrations increased the total actin content, actin fiber thickness, and cytoplasmic membrane roughness, with significant increase in Runx2 mRNA levels. These results show that bone marrow stem cells demonstrate similar cytoskeletal adaptations to low-magnitude high-frequency mechanical loads both during quiescence and osteogenesis, potentially becoming more sensitive to additional loads by increased structural stiffness.

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“…Interestingly, induction of miR21 was found to accelerate osteogenesis more in the SS population than in RS cells [71] . Finally, human AFMSCs analyzed by an atomic force microscope during osteogenic differentiation showed a decrease in cell elasticity, which is typical of mature osteoblasts; thus the mechanical properties of AFMSCs again add to the interest in applying them in bone regenerative medicine [72] . Up to now, little is known about the cues regulating the AFMSCs' ability to differentiate to osteoblasts.…”

Section: In Vitro Osteogenic Differentiationmentioning

confidence: 94%

Osteogenic differentiation of amniotic fluid mesenchymal stromal cells and their bone regeneration potential

Pipino

Pandolfi

2015

WJSC

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AFM Studies of Cellular Mechanics during Osteogenic Differentiation of Human Amniotic Fluid-derived Stem Cells

Cited by 28 publications

References 25 publications

Morphological and Mechanical Properties of Osteosarcoma Microenvironment Cells Explored by Atomic Force Microscopy

Morphological and Mechanical Properties of Osteosarcoma Microenvironment Cells Explored by Atomic Force Microscopy

Bone marrow stem cells adapt to low-magnitude vibrations by altering theircytoskeleton during quiescence and osteogenesis

Osteogenic differentiation of amniotic fluid mesenchymal stromal cells and their bone regeneration potential

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