AFM membrane roughness as a probe to identify oxidative stress-induced cellular apoptosis

Wang, Dau-Chung; Chen, Ken-Yen; Tsai, Cheng‐Hsien; Chen, Gen-You; Chen, Chuan-Hung

doi:10.1016/j.jbiomech.2011.08.021

Cited by 41 publications

(25 citation statements)

References 27 publications

(25 reference statements)

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“…The images shown are a typical representation of these changes. The obvious morphological changes such as disappearance of filopodia, volume changes, and transformation to a round shape observed by AFM were consistent through three separate experiments only in the area of laser illumination (Wang et al 2011b;Lee et al 2013;Gaspar et al 2015). These results suggest that the combination of nanoparticles and radiation triggers cell death by apoptosis in attached cells.…”

Section: Laser Treatment Of Tumor Cells With Pani-npssupporting

confidence: 76%

Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells

et al. 2015

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Polyaniline nanoparticles (PANI-Nps) have been used in several applications; however, there are few publications related to the use in the photothermal therapy. PANI-Nps have high optical absorbance in the near-infrared region and in this wavelength range, biological systems are relatively transparent. For this reason, these materials can be used to absorb energy and to generate heat that destroys cancer cells selectively. PANI-Nps with average size of ca. 200 nm and neutral zeta potential were synthesized and characterized by DLS, SEM, and zeta potential. The kinetics of incorporation of PANINps into LM2 cell line was monitored using UV-Vis spectrophotometry. The analysis of cell viability after PANI-Nps exposure shows that these nanoparticles are not cytotoxic even at high concentration and show no change in cell morphology and metabolic activity. Furthermore, we found that nanoparticle cell uptake reaches the maximum value c.a. 3 h after incubation. Cells were targeted by Pani-Nps and irradiated, resulting in significant elevation of intracellular ROS and heat production. One of the mechanisms of PANINps-mediated photothermal killing of cancer cells apparently involved oxidative stress resulting in apoptotic cell death.

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Section: Laser Treatment Of Tumor Cells With Pani-npssupporting

confidence: 76%

Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells

et al. 2015

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“…When performing AFM imaging on living cells, the soft and dynamic nature of cell membrane makes that the tip can easily deform the cell surface [35] which introduces artificial errors if we directly evaluate cell roughness based on the images on living cells. Hence, current studies about measuring the cellular surface roughness by AFM are commonly performed on fixed cells [33], [34], [36]. However, chemical fixation inevitably alters the structures of cells.…”

Section: Resultsmentioning

confidence: 99%

Nanoscale Quantifying the Effects of Targeted Drug on Chemotherapy in Lymphoma Treatment Using Atomic Force Microscopy

Xiao

Liu

et al. 2016

IEEE Trans. Biomed. Eng.

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Abstract-The applications of targeted drugs in treating cancers have significantly improved the survival rates of patients. However, in the clinical practice, targeted drugs are commonly combined with chemotherapy drugs, causing that the exact contribution of targeted drugs to the clinical outcome is difficult to evaluate. Quantitatively investigating the effects of targeted drugs on chemotherapy drugs on cancer cells is useful for us to understand drug actions and design better drugs. The advent of atomic force microscopy (AFM) provides a powerful tool for probing the nanoscale physiological activities of single live cells. In this paper, the detailed changes in cell morphology and mechanical properties were quantified on single lymphoma cells during the actions of rituximab (a monoclonal antibody targeted drug) and two chemotherapy drugs (cisplatin and cytarabine) by AFM. AFM imaging revealed the distinct changes of cellular ultramicrostructures induced by the drugs. The changes of cellular mechanical properties after the drug stimulations were measured by AFM indenting. The statistical histograms of cellular surface roughness and mechanical properties quantitatively showed that rituximab could remarkably strengthen the killing effects of chemotherapy drugs. The study offers a new way to quantify the synergistic interactions between targeted drugs and chemotherapy drugs at the nanoscale, which will have potential impacts on predicting the efficacies of drug combinations before clinical treatments.

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“…31 Therefore, any change in the cell membrane structure can be a sensitive indicator of the state of a cell, including normal growth conditions and death. With respect to the morphological changes associated with cellular apoptosis, 22 we inferred that the increased roughness was likely due to membrane damage incurred by apoptosis.…”

Section: Discussionmentioning

confidence: 98%

Investigation of biochemical property changes in activation-induced CD 8 + T cell apoptosis using Raman spectroscopy

et al. 2015

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Abstract. The study was to investigate the changes in biochemical properties of activated mature CD8þ T cells related to apoptosis at a molecular level. We confirmed the activation and apoptosis of CD8þ T cells by fluorescence-activated cell sorting and atomic force microscopy and then performed Raman spectral measurements on activated mature CD8þ T cells and cellular deoxyribose nucleic acid (DNA). In the activated mature CD8þ T cells, there were increases in protein spectra at 1002 and 1234 cm −1 . In particular, to assess the apoptosisrelated DNA spectral signatures, we investigated the spectra of the cellular DNA isolated from resting and activated mature CD8þ T cells. Raman spectra at 765 to 786 cm −1 and 1053 to 1087 cm −1 were decreased in activated mature DNA. In addition, we analyzed Raman spectrum using the multivariate statistical method including principal component analysis. Raman spectra of activated mature DNA are especially well-discriminated from those of resting DNA. Our findings regarding the biochemical and structural changes associated with apoptosis in activated mature T cells and cellular DNA according to Raman spectroscopy provide important insights into allospecific immune responses generated after organ transplantation, and may be useful for therapeutic manipulation of the immune response. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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AFM membrane roughness as a probe to identify oxidative stress-induced cellular apoptosis

Cited by 41 publications

References 27 publications

Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells

Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells

Nanoscale Quantifying the Effects of Targeted Drug on Chemotherapy in Lymphoma Treatment Using Atomic Force Microscopy

Investigation of biochemical property changes in activation-induced CD 8 + T cell apoptosis using Raman spectroscopy

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