AFLP and single-strand conformation polymorphism studies of recombination in the entomopathogenic fungus Nomuraea rileyi

Devi, Uma K.; Reineke, Annette; Rao, Uma; Reddy, Nageswara Rao N.; Khan, Akbar P. Ali

doi:10.1016/j.mycres.2007.03.003

Cited by 21 publications

(4 citation statements)

References 43 publications

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“…Some parasite species may have host-based genetic structure, like Nomuraea rileyi [90] , and some insects with a strong ability of migration also depend on their host plants [36] . This even holds true for some vertebrate animals like certain bird species [91] .…”

Section: Discussionmentioning

confidence: 99%

Population Explosion in the Yellow-Spined Bamboo Locust Ceracris kiangsu and Inferences for the Impact of Human Activity

et al. 2014

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Geographic distance and geographical barriers likely play a considerable role in structuring genetic variation in species, although some migratory species may have less phylogeographic structure on a smaller spatial scale. Here, genetic diversity and the phylogenetic structure among geographical populations of the yellow-spined bamboo locust, Ceracris kiangsu, were examined with 16S rDNA and amplified fragment length polymorphisms (AFLPs). In this study, no conspicuous phylogeographical structure was discovered from either Maximum parsimony (MP) and Neighbor-joining (NJ) phylogenetic analyses. The effect of geographical isolation was not conspicuous on a large spatial scale.At smaller spatial scales local diversity of some populations within mountainous areas were detected using Nei's genetic distance and AMOVA. There is a high level of genetic diversity and a low genetic differentiation among populations in the C. kiangsu of South and Southeast China. Our analyses indicate that C. kiangsu is a monophyletic group. Our results also support the hypothesis that the C. kiangsu population is in a primary differentiation stage. Given the mismatch distribution, it is likely that a population expansion in C. kiangsu occurred about 0.242 Ma during the Quaternary interglaciation. Based on historical reports, we conjecture that human activities had significant impacts on the C. kiangsu gene flow.

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Section: Discussionmentioning

confidence: 99%

Population Explosion in the Yellow-Spined Bamboo Locust Ceracris kiangsu and Inferences for the Impact of Human Activity

et al. 2014

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“…This technique has been widely used in species identification and differentiation of human-, insect-, and plant-pathogenic fungi [12]–[15]; mycorrhizal fungi [16]; and yeasts [17]. This technique has also been used to study intraspecific genetic diversity of fungi [18]–[23]. As a community profiling technique, SSCP has also been applied to bacterial and fungal communities [24]–[26].…”

Section: Introductionmentioning

confidence: 99%

High Diversity of the Fungal Community Structure in Naturally-Occurring Ophiocordyceps sinensis

Zhang

Wang

et al. 2010

PLoS ONE

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Background Ophiocordyceps sinensis (syn. Cordyceps sinensis), which is a parasite of caterpillars and is endemic to alpine regions on the Tibetan Plateau, is one of the most valuable medicinal fungi in the world. “Natural O. sinensis specimens” harbor various other fungi. Several of these other fungi that have been isolated from natural O. sinensis specimens have similar chemical components and/or pharmaceutical effects as O. sinensis. Nevertheless, the mycobiota of natural O. sinensis specimens has not been investigated in detail.Methodology/Principal FindingsBased on the technique of PCR-single-strand conformation polymorphism (PCR-SSCP), the mycobiota of three different sections (stromata, sclerotia, and mycelial cortices) from natural O. sinensis specimens were investigated using both culture-dependent and -independent methods. For the culture-dependent method, 572 fungal strains were isolated, and 92 putative operational taxonomic units (OTUs) were identified from 226 sequenced strains with the threshold of 97%. For the culture-independent method, 490 fungal clones were identified from about 3000 clones of ITS fragments from the whole-community DNA; based on PCR-SSCP analyses, 266 of these clones were selected to be sequenced, and 118 putative OTUs were detected. The overwhelming majority of isolates/clones and OTUs were detected from mycelial cortices; only a few were detected from stromata and sclerotia. The most common OTUs detected with both methods belonged to Ascomycota; however, only 13 OTUs were detected simultaneously by both methods. Potential novel lineages were detected by each of the two methods.Conclusions/SignificanceA great number of fungal species present in the mycobiota of naturally-occurring O. sinensis specimens were detected, and many of them may represent undescribed lineages. That only a few of the same OTUs were detected by both methods indicated that different methods should be used. This study increased our understanding about the fungal community structure of this valuable medicinal herb.

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“…The main susceptible species of insects, which are key pests of crops such as cotton and soybean, belong to the lepidopteran families Noctuidae, Erebidae, and Nymphalidae (3–7). Metarhizium rileyi usually presents high genetic variability (8), which has been closely related to the host species from which it is isolated (4, 9, 10). Unlike other most common fungal entomopathogens with the greatest known epizootic potentials, such as Metarhizium anisopliae and Beauveria bassiana, M.…”

Section: Announcementmentioning

confidence: 99%

Genome Sequence of Metarhizium rileyi , a Microbial Control Agent for Lepidoptera

Binneck

Lastra

Sosa–Gómez

2019

Microbiol Resour Announc

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AFLP and single-strand conformation polymorphism studies of recombination in the entomopathogenic fungus Nomuraea rileyi

Cited by 21 publications

References 43 publications

Population Explosion in the Yellow-Spined Bamboo Locust Ceracris kiangsu and Inferences for the Impact of Human Activity

Population Explosion in the Yellow-Spined Bamboo Locust Ceracris kiangsu and Inferences for the Impact of Human Activity

High Diversity of the Fungal Community Structure in Naturally-Occurring Ophiocordyceps sinensis

Genome Sequence of Metarhizium rileyi , a Microbial Control Agent for Lepidoptera

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