Aflatoxin B1 causes oxidative stress and apoptosis in sheep testes associated with disrupting rumen microbiota

Lin, Luxi; Cao, Qi; Zhang, Chaodong; Xu, Tingting; Ke, Yanxiong; Li, Qinghao; Liu, Fang; Wang, Xuebing; Dong, Hui; Huang, Shucheng; Jian, Fuchun

doi:10.1016/j.ecoenv.2022.113225

Cited by 30 publications

(10 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, AFB 1 exposure decreased spermatogenesis in mice by inducing oxidative stress, decreasing mitochondrial content, and upregulating the pro-apoptotic Bax, p53 and Caspase 3 ( Yasin, Mazdak & Mino, 2018 ). AFB 1 also induced testicular damage and testicular dysfunction in Dorper rams ( Lin et al, 2022 ), and impaired spermatogenesis in white leghorn cockerels ( Ashraf et al, 2022 ). Exposure to low concentrations of AFB 1 for several hours can decrease spermatozoa motility, hyperpolarize mitochondrial membranes, and increase DNA fragmentation ( Komsky-Elbaz, Saktsier & Roth, 2018 ).…”

Section: Afb 1 -Induced Toxicitymentioning

confidence: 99%

Research progress in toxicological effects and mechanism of aflatoxin B₁ toxin

Liu

et al. 2022

PeerJ

View full text Add to dashboard Cite

Fungal contamination of animal feed can severely affect the health of farm animals, and result in considerable economic losses. Certain filamentous fungi or molds produce toxic secondary metabolites known as mycotoxins, of which aflatoxins (AFTs) are considered the most critical dietary risk factor for both humans and animals. AFTs are ubiquitous in the environment, soil, and food crops, and aflatoxin B1(AFB1) has been identified by the World Health Organization (WHO) as one of the most potent natural group 1A carcinogen. We reviewed the literature on the toxic effects of AFB1 in humans and animals along with its toxicokinetic properties. The damage induced by AFB1 in cells and tissues is mainly achieved through cell cycle arrest and inhibition of cell proliferation, and the induction of apoptosis, oxidative stress, endoplasmic reticulum (ER) stress and autophagy. In addition, numerous coding genes and non-coding RNAs have been identified that regulate AFB1 toxicity. This review is a summary of the current research on the complexity of AFB1 toxicity, and provides insights into the molecular mechanisms as well as the phenotypic characteristics.

show abstract

Section: Afb 1 -Induced Toxicitymentioning

confidence: 99%

Research progress in toxicological effects and mechanism of aflatoxin B₁ toxin

Liu

et al. 2022

PeerJ

View full text Add to dashboard Cite

show abstract

“…AFB1 is the most toxic of all aflatoxins, which can induce oxidative stress in the testes, causing pathological damage and dysfunction of the testes [ 69 ]. It has been reported that AFB1 was able to significantly increase the production of intracellular ROS and decrease the level of glutathione, thereby activating several signal pathways related to inflammatory response to induce oxidative stress in cells [ 70 ].…”

Section: Major Toxicity Mechanisms Induced By Mycotoxins and Heavy Me...mentioning

confidence: 99%

Detoxification of Selenium Yeast on Mycotoxins and Heavy Metals: a Review

Sun

Chen

Xiong

et al. 2023

Biol Trace Elem Res

View full text Add to dashboard Cite

Mycotoxins are secondary metabolites produced by specific fungi. More than 400 different mycotoxins are known in the world, and the concentration of these toxins in food and feed often exceeds the acceptable limit, thus causing serious harm to animals and human body. At the same time, modern industrial agriculture will also bring a lot of environmental pollution in the development process, including the increase of heavy metal content, and often the clinical symptoms of low/medium level chronic heavy metal poisoning are not obvious, thus delaying the best treatment opportunity. However, the traditional ways of detoxification cannot completely eliminate the adverse effects of these toxins on the body, and sometimes bring some side effects, so it is essential to find a new type of safe antidote. Trace element selenium is among the essential mineral nutrient elements of human and animal bodies, which can effectively remove excessive free radicals and reactive oxygen species in the body, and has the effects of antioxidant, resisting stress, and improving body immunity. Selenium is common in nature in inorganic selenium and organic selenium. In previous studies, it was found that the use of inorganic selenium (sodium selenite) can play a certain protective role against mycotoxins and heavy metal poisoning. However, while it plays the role of antioxidant, it will also have adverse effects on the body. Therefore, it was found in the latest study that selenium yeast could not only replace the protective effect of sodium selenite on mycotoxins and heavy metal poisoning, but also improve the immunity of the body. Selenium yeast is an organic selenium source with high activity and low toxicity, which is produced by selenium relying on the cell protein structure of growing yeast. It not only has high absorption rate, but also can be stored in the body after meeting the physiological needs of the body for selenium, so as to avoid selenium deficiency again in the short term. However, few of these studies can clearly reveal the protective mechanism of yeast selenium. In this paper, the detoxification mechanism of selenium yeast on mycotoxins and heavy metal poisoning was reviewed, which provided some theoretical support for further understanding of the biological function of selenium yeast and its replacement for inorganic selenium. The conclusions suggest that selenium yeast can effectively alleviate the oxidative damage by regulating different signaling pathways, improving the activity of antioxidant enzymes, reversing the content of inflammatory factors, regulating the protein expression of apoptosis-related genes, and reducing the accumulation of mycotoxins and heavy metals in the body.

show abstract

“…Oxidative stress is one of the critical molecular mechanisms of adverse effects caused by many toxic chemical hazards or drugs (e.g., heavy metals, mycotoxins, pesticides, chemotherapy drugs, and antibiotics) [ 88 ]. Multiple studies reported that AFB1 and its derivatives could trigger oxidative stress by inducing the formation of ROS or decreasing intracellular antioxidants or the activities of antioxidant enzymes [ 36 , 70 , 73 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 ]. In the liver tissues, the cytochrome (CYP)1A2 and CYP3A4 could bio-transform into AFBO, which is a harmful and extremely active, electrophilic metabolite.…”

Section: Curcumin’s Protective Role In Preventing Afb1-induced Toxici...mentioning

confidence: 99%

“…The formation of mitochondrial outer membrane permeabilization could facilitate the release of CytC from the mitochondria to the cytoplasm in a cascade to activate caspases-9, -3 and cleaved PARP1, finally causing apoptotic cell death [ 144 ]. Some investigations found that AFB1 exposure increased Bax protein expression while decreasing Bcl-2 protein expression, resulting in the release of the CytC form from the mitochondria, the activation of caspases-9 and -3, and cell apoptosis [ 40 , 47 , 70 , 89 , 90 , 95 , 137 , 145 ]. In addition, AFB1 exposure could also trigger other signaling pathways, including p53, MAPKs, and Akt pathways, which play a critical role in regulating the mitochondrial apoptotic pathway [ 146 , 147 ].…”

Section: Curcumin’s Protective Role In Preventing Afb1-induced Toxici...mentioning

confidence: 99%

Aflatoxin B1 Toxicity and Protective Effects of Curcumin: Molecular Mechanisms and Clinical Implications

et al. 2022

View full text Add to dashboard Cite

One of the most significant classes of mycotoxins, aflatoxins (AFTs), can cause a variety of detrimental outcomes, including cancer, hepatitis, aberrant mutations, and reproductive issues. Among the 21 identified AFTs, aflatoxin B1 (AFB1) is the most harmful to humans and animals. The mechanisms of AFB1-induced toxicity are connected to the generation of excess reactive oxygen species (ROS), upregulation of CYP450 activities, oxidative stress, lipid peroxidation, apoptosis, mitochondrial dysfunction, autophagy, necrosis, and inflammatory response. Several signaling pathways, including p53, PI3K/Akt/mTOR, Nrf2/ARE, NF-κB, NLRP3, MAPKs, and Wnt/β-catenin have been shown to contribute to AFB1-mediated toxic effects in mammalian cells. Curcumin, a natural product with multiple therapeutic activities (e.g., anti-inflammatory, antioxidant, anticancer, and immunoregulation activities), could revise AFB1-induced harmful effects by targeting these pathways. Therefore, the potential therapeutic use of curcumin against AFB1-related side effects and the underlying molecular mechanisms are summarized. This review, in our opinion, advances significant knowledge, sparks larger discussions, and drives additional improvements in the hazardous examination of AFTs and detoxifying the application of curcumin.

show abstract

Aflatoxin B1 causes oxidative stress and apoptosis in sheep testes associated with disrupting rumen microbiota

Cited by 30 publications

References 52 publications

Research progress in toxicological effects and mechanism of aflatoxin B₁ toxin

Research progress in toxicological effects and mechanism of aflatoxin B₁ toxin

Detoxification of Selenium Yeast on Mycotoxins and Heavy Metals: a Review

Aflatoxin B1 Toxicity and Protective Effects of Curcumin: Molecular Mechanisms and Clinical Implications

Contact Info

Product

Resources

About

Aflatoxin B1 causes oxidative stress and apoptosis in sheep testes associated with disrupting rumen microbiota

Cited by 30 publications

References 52 publications

Research progress in toxicological effects and mechanism of aflatoxin B1 toxin

Research progress in toxicological effects and mechanism of aflatoxin B1 toxin

Detoxification of Selenium Yeast on Mycotoxins and Heavy Metals: a Review

Aflatoxin B1 Toxicity and Protective Effects of Curcumin: Molecular Mechanisms and Clinical Implications

Contact Info

Product

Resources

About

Research progress in toxicological effects and mechanism of aflatoxin B₁ toxin

Research progress in toxicological effects and mechanism of aflatoxin B₁ toxin