Affinity sorbent based on tryptophyl-threonyl-tyrosine for binding of the immunoglobulins G: Sorption characteristics and aspects of practical application

Abstract

New chromatographic material based on tryptophil-threonil-tirosine was prepared. This sorbent effectively binds human, sheep, goat and cow immunoglobulins G. New sorbent shows high selectivity for removing immunoglobulins from blood plasma. Effective sorption capacity is 15-25 mg of immunoglobulin G per ml of matrix. Optimal method of covalent attachment ligand to polysaccharide matrix allows achieving high stability of the sorbents in terms of use and storage. This sorbent can be used in medicine and biotechnology.

Covalently immobilized chemically modified lysozyme as a sorbent for bacterial endotoxins (lipopolysaccharides)

“…Protein concentration was determined via the Bradford method with Coomassie G250 [17] and by microbiuret method [18] with a modified Benedict reagent [19].…”