DNA aptamer-based sorbents for binding human IgE

Abstract

DNA aptamer based sorbents are synthesized for binding human IgE. Sorbents effectively removed IgE from human blood plasma. The experimental values of IgE desorption constants were from 11 x 10(-l0) to 1.7 x 10(-10) M depending on the orientation of the aptamer, an insoluble matrix. The sorbents were stable during multiple use. Conditions for sorbent regeneration were picked up. These chromatographic materials can be used for medical and biotechnological applications.

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

“…The variant SELEX exists to enhance oligonucleotides conformation stability or resistance to nucleases depending on target molecule. Some of them are genomic SELEX or cDNA-SELEX [37], photo SELEX [38], covalent-SELEX or cross-linking SELEX [39][40][41], multistage SELEX [42] used for starting pool rationalization or improve aptamer selectivity using negative SELEX protocol or counter SELEX, and deconvolution SELEX or subtractive SELEX. These help to remove undesirable adsorbed oligonucleotides from the poolby matrixes that used for immobilizing the targets and distinction of similar structures [43].…”

An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

“…Other changes include target immobilization and nucleic acid library expension (McKeague and Derosa 2012). For oligonucleotide stability and resistance against nuclease enhancement, several SELEX protocols have been developed such as genomic SELEX or cDNA-SELEX (Zimmermann et al 2010), covalent SELEX (Kopylov and Spiridonova 2000;Spiridonova 2014;Spiridonova et al 2014), deconvolution SELEX or subtractive SELEX (Torres-Chavolla and Alocilja 2009), photo SELEX (Cho et al 2004), and multistage SELEX (modified chimeric SELEX) (Wu and Curran 1999) for rationalization of starting pool. Counter SELEX or negative SELEX protocol in aptamer is for selectivity enhancement (Xi et al 2014).…”