Affinity processes realized on high-flow-through methacrylate-based macroporous monoliths

Platonova, G. A.; Tennikova, Tatiana

doi:10.1016/j.chroma.2004.12.008

Cited by 41 publications

(21 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This includes the stability over a wide pH range considering strong changes in pH for elution, a low nonspecific binding, and the provision of functional groups for ligand immobilization 3. Over the last few years, supports based on organic monoliths have become more popular, which is reflected in publication of several reviews dealing with various applications of monolithic supports in affinity chromatography 4–12. Most organic monoliths such as commonly used support materials based on agarose are stable over a large pH range.…”

Section: Introductionmentioning

confidence: 99%

Oligoepoxide‐Based Monoliths: Synthesis and Application as Affinity Capillary Column for Enrichment of Immunoglobulin G

Pecher

Zimathies

Weller

2012

Macro Chemistry & Physics

View full text Add to dashboard Cite

A series of new epoxide‐based monoliths is synthesized by self‐polymerization of polyglycerol polyglycidyl ether as porous support for affinity chromatography. Porosity and mean pore sizes are investigated as a function of the porogen ratio and analyzed by mercury porosimetry. The morphologies are visualized by environmental scanning electron microscope. With a particularly rigid monolith prepared with 75 vol% porogen (66% porosity and 12 μm mean pore size), a capillary column (0.5 mm ID) is prepared and directly functionalized with recombinant protein A to extract immunoglobulin G from rabbit serum.

show abstract

Section: Introductionmentioning

confidence: 99%

Oligoepoxide‐Based Monoliths: Synthesis and Application as Affinity Capillary Column for Enrichment of Immunoglobulin G

Pecher

Zimathies

Weller

2012

Macro Chemistry & Physics

View full text Add to dashboard Cite

show abstract

“…The method of affinity chromatography on monoliths allows carrying out the isolation, purification and fractionation of complex biological mixtures in fast elution mode [7,9,27].…”

Section: Calculate Coupling Efficiency (See Note 3)mentioning

confidence: 99%

“…Nowadays, the monoliths produced on the base of natural or synthetic polymers, as well as inorganic or hybrid materials are known from current literature [2][3][4][5]. Among different types of monoliths, methacrylate-based monolithic columns, which, in fact, still represent the only commercial product, are the most frequently used for immobilization of various ligands that is quite useful for modern high-speed affinity chromatography [6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Affinity Chromatography of Proteins on Monolithic Columns

Влах

Platonova

Tennikova

2014

Methods in Molecular Biology

View full text Add to dashboard Cite

At present, monolithic stationary phases, because of their morphology, are widely used for development and realization of fast dynamic and static processes based on mass transition between liquid and solid phases. These are liquid chromatography, solid phase synthesis, microarrays, flow-through enzyme reactors, etc. High-performance liquid chromatography on monoliths, including bioaffinity mode, represents a unique technique appropriate for fast and efficient separation of biological (macro)molecules of different sizes and shapes (proteins, nucleic acids, peptides), as well as such supramolecular systems as viruses.In this work, the examples of application of commercially available macroporous monoliths for modern affinity processing are presented. In particular, the original methods developed for efficient isolation and fractionation of monospecific antibodies from rabbit blood sera, the possibility of simultaneous affinity separation of protein G and serum albumin from human serum, the isolation of recombinant products, such as protein G and tissue plasminogen activator from E. coli cell lysate and Chinese Hamster Ovary cell culture supernatant, respectively, are described in detail. The suggested and realized multifunctional fractionation of polyclonal pools of antibodies by combination of several short monolithic columns (disks) with different affinity functionalities stacked in the same cartridge represents an original and practically valuable method that can be used in biotechnology.

show abstract

“…The internal structure consists of aggregates of polymeric microglobules interconnected by pores; their stiffness results from the high degree of the crosslinking they possess. They are generally particulate materials prepared from a suspension polymerization reaction 14. Beyond the strengths of these media, particulate materials have two major limitations: the large volume interparticle and the slow diffusion of high molecular weight solutes (proteins, polysaccharides or synthetic polymers) into the pores of the particles by their low diffusion coefficients.…”

Section: Macroporous Materialsmentioning

confidence: 99%

Macroporous monolithic supports for affinity chromatography

Arrua

Igarzabal

2011

J of Separation Science

View full text Add to dashboard Cite

In the early 1990s, three research groups simultaneously developed continuous macroporous rod-shaped polymeric systems to eliminate the problem of flow through the interparticle spaces generally presented by the chromatography columns that use particles as filler. The great advantage of those materials, forming a continuous phase rod, is to increase the mass transfer by convective transport, as the mobile phase is forced to go through all means of separation, in contrast to particulate media where the mobile phase flows through the interparticle spaces. Due to their special characteristics, the monolithic polymers are used as base-supports in different separation techniques, those chromatographic processes being the most important and, to a greater extent, those involving the separation of biomolecules as in the case of affinity chromatography. This mini-review reports the contributions of several groups to the development of macroporous monoliths and their modification by immobilization of specific ligands on the products for their application in affinity chromatography.

show abstract

Affinity processes realized on high-flow-through methacrylate-based macroporous monoliths

Cited by 41 publications

References 68 publications

Oligoepoxide‐Based Monoliths: Synthesis and Application as Affinity Capillary Column for Enrichment of Immunoglobulin G

Oligoepoxide‐Based Monoliths: Synthesis and Application as Affinity Capillary Column for Enrichment of Immunoglobulin G

Affinity Chromatography of Proteins on Monolithic Columns

Macroporous monolithic supports for affinity chromatography

Contact Info

Product

Resources

About