Affinity biosensors based on solid supported lipid membranes. Their structure, physical properties and dynamics

Hianik, Tibor; Passechnik, V.I.; Sokolikova, L.; Šnejdárková, Maja; Sivak, Branislav; Fajkus, Martin; Ivanov, S. A.; Fránek, Milan

doi:10.1016/s0302-4598(98)00162-7

Cited by 25 publications

(9 citation statements)

References 38 publications

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“…The direct monitoring of small molecules was studied using several techniques including the effect of conformation changes during interaction with the corresponding antibodies. Antibody attached to bilayer lipid membrane on the surface of a conductometric sensor was used for detection of 2,4-D [9]. The conformation changes of metallothioneins immobilized on self-assembled thiol layers were used for subnanomolar detection of heavy metals using capacitance measurements [10].…”

Section: Introductionmentioning

confidence: 99%

The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy

Navrátilová

Skládal

2004

Bioelectrochemistry

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Section: Introductionmentioning

confidence: 99%

The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy

Navrátilová

Skládal

2004

Bioelectrochemistry

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“…C K is the capacitance of the connected cables. The value of C M for s-BLM on alkylthiol support is similar to free standing solvent-free membranes and is approximately 0.8 mF=cm 2 [17,20], while the C S value is about 10 times higher [24], i.e. ca.…”

Section: ±10mentioning

confidence: 74%

“…The methods of impedance, conductance and capacitance relaxation were used for study of the physical properties of the supported membranes and for determination of hybridization. The method of capacitance relaxation was recently shown as being informative for the study of the antigen-antibody reaction at the surface of supported lipid ®lms [17].…”

Section: Introductionmentioning

confidence: 99%

The Changes in Dynamics of Solid Supported Lipid Films Following Hybridization of Short Sequence DNA

et al. 2000

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The methods of conductance and capacitance relaxation were used to monitor hybridization of short sequences of DNA on the surface of lipid membranes (s-BLM) supported on thin platinum or gold layers covered by 1-dodecanethiol. The 15-mer-5H -hexadecyl-deoxythymidylic modi®ed by palmitic acid (C 16 pdT 15 ) was used as a DNA sensor that was incorporated into the BLM by the C 16 chain. The interaction of C 16 pdT 15 with s-BLM resulted in decrease membrane capacitance and conductance. The DNA hybridization on s-BLM surface resulted in an increase of s-BLM conductance, however, interaction of s-BLM modi®ed by C 16 pdT 15 with noncomplementary oligonucleotide did not change membrane conductance. The capacitance relaxation following symmetrical voltage jumps allowed us to study the dynamics of the reorientation dipole moments of the membrane. The unmodi®ed s-BLM were characterized by two relaxation components: fast t 1 20.3 AE 0.2 ms and slow t 2 590.3AE 175 ms. Modi®cation of the lipid layer by C 16 pdT 15 resulted in a decrease of amplitude of the slower component below detectable limit, while hybridization resulted in recovery of two relaxation times. Similar behavior was characterized for the free standing BLM. The changes of relaxation times might be due to the in¯uence of the hybridization process on the two-dimensional architecture of the phospholipid layer, for example on the size of the lipid clusters.

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“…These ®lms were characterized by higher conductivity in the gel state rather than in the liquid-crystalline state, which can be explained by an increased amount of structural defect of the gel state of sBLM [6]. In our recent work [47], we observed frequency dependence of the speci®c capacitance of sBLM formed on gold layers [®rst monolayer adjacent to thin smooth gold layer was 1-dodecanethiol, while the second monolayer was from crude ox brain fraction (COB)]. However, in contrast to sBLMs formed on the rather rough surface of the tip of stainless steel wire, the C s of sBLMs on gold layers were characterized by the dispersion with frequency only at f 41 kHz for ionic strength lower than 0.3 mM and at f 410 kHz for an ionic strength of 0.1 M. The speci®c capacitance for a frequency range of 100 Hz±1 kHz was ca.…”

Section: Specific Conductivity Capacitance and Stabilitymentioning

confidence: 87%

Biosensors Based on Thin Lipid Films and Liposomes

Nikolelis¹,

Hianik

Krull³

1999

Electroanalysis

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This article reports the theory and analytical applications of thin lipid ®lms. Recent advances of electrochemical devices based on lipid membranes have lead to reports of construction of biosensors for environmental and food applications, and may provide opportunities for commercial fabrication. The methods of formation of lipid membranes on various supports including metals (silver, gold, stainless steel), agar, conducting polymers and ultra®ltration membranes have provided stabilization of lipid ®lms with a diversity of analytical applications in real samples. Methods of immobilization and incorporation of various functional macromolecules are summarized. Several examples of the application of various methods for study of physical properties of supported bilayer lipid membranes are described. Applications of lipid-based biosensors in analytical chemistry for determination of compounds are demonstrated, including a diversity of chemical compounds such as environmental pollutants (ammonia and carbon dioxide, cyanide ions, etc.) and food toxins (a¯atoxin M 1 and direct detection of toxin in real samples such as milk and milk preparations). Methods for application of liposomes as a sensing system are also summarized.

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Affinity biosensors based on solid supported lipid membranes. Their structure, physical properties and dynamics

Cited by 25 publications

References 38 publications

The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy

The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy

The Changes in Dynamics of Solid Supported Lipid Films Following Hybridization of Short Sequence DNA

Biosensors Based on Thin Lipid Films and Liposomes

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