Affinity biosensors based on disposable screen-printed electrodes modified with DNA

Evtugyn, Gennady; Mingaleva, Anastasia; Budnikov, H. C.; Stoikova, E. E.; Винтер, В. Г.; Eremin, Sergei A.

doi:10.1016/s0003-2670(02)01540-4

Cited by 26 publications

(13 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taking into account the ability of sulfonamides and their metabolites to form adducts with ds-DNA [23,25], we decided to explore the possibility of application of the FI methodology described above to the detection of sulfonamides. Fig.…”

Section: Use Of the Dna Sensor Under Flow Injection Conditionsmentioning

confidence: 99%

“…On the other hand, it is also well known that the interaction of DNA with low-molecular weight compounds can be used for the determination of drugs such as nitroimidazole effectors, anticancer drugs, sulfonamides, or anthracyclines [23][24][25][26]. Moreover, the detection of chemicals that can cause irreversible damage to DNA in the cells is considered of great importance, as it often results in mutations that may lead to hereditary or carcinogenic diseases [27,28].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the detection of chemicals that can cause irreversible damage to DNA in the cells is considered of great importance, as it often results in mutations that may lead to hereditary or carcinogenic diseases [27,28]. One of the approaches used with this goal implies the use of electroactive substances interacting with DNA covalently and non-covalently, such as phenothiazine dyes like MB [23]. Thus, Evtugyn et al recently described a novel DNA sensor based on a glassy carbon electrode modified with ds-DNA and HRP and demonstrated that the biosensor signal related to HRP oxidation of MB and methylene green depends on the nature and concentration of the pharmaceuticals added to the reaction medium [25].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Amperometric DNA quantification based on the use of peroxidase-mercaptopropionic acid-modified gold electrodes

Loaiza¹,

Campuzano²,

Prada³

et al. 2008

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

Section: Use Of the Dna Sensor Under Flow Injection Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Amperometric DNA quantification based on the use of peroxidase-mercaptopropionic acid-modified gold electrodes

Loaiza¹,

Campuzano²,

Prada³

et al. 2008

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

“…The binding of specific antibodies resulted in the decrease of the rate of enzymatic reaction due to the suppression of the substrate delivery from the bulk solution. The apparent inhibition of enzyme activity was recorded amperometrically in DC mode [18,19]. The appropriate DNA-sensors were successfully applied for diagnostics of human systemic lupus erythemathosus and Aleutian disease of mink.…”

Section: Introductionmentioning

confidence: 99%

Potentiometric DNA Sensor Based on Electropolymerized Phenothiazines for Protein Detection

et al. 2008

View full text Add to dashboard Cite

Novel method of potentiometric detection of DNA-protein interactions has been proposed. For this purpose, polymeric phenothiazine dyes, methylene blue (MB) and methylene green (MG), were electrochemically deposited onto the glassy carbon electrode and covered with double stranded DNA (dsDNA) as a target for antibodies (DNAsensor) or DNA aptamer specific to human a-thrombin (aptasensor). The biosensors were consecutively incubated at pH 7.5 and 3.0 and the difference in potentials, DE, was used as a measure of protein concentration. The potentiometric DNA-sensors were tested in standard serum of autoimmune disease patients (systemic lupus erythemathosus (SLE) and autoimmune thyroidites). It was shown, that the DE value of DNA-sensor depends on the dilution of serum in the range from 1 : 1 to 1 : 100. Nonthermostated serum exhibited bell-shape dependence of DE on serum dilution due to interfering effect of serine proteins at maximum dilution between 1 : 20 and 1 : 50. For SLE serum thermostated at 56 8C the DE linearly decreased as a function of serum dilution and reached saturation at dilution 1 : 20. Similarly the changes in the potential of aptasensor allowed us to determine the a-thrombin in the range from 1 nM to 1 mM. The Faradic impedance spectra measured at presence of redox probe [Fe(CN) 6 ] 4À/3À revealed changes in the resistance and capacitance attributed to the shielding effect of anti-DNA antibodies and an increase in the electron transfer. The developed potentiometric biosensors can be used for preliminary diagnostics of autoimmune diseases and thrombin detection with sensitivity comparable to traditional methods. The developed assay is, however simpler and cheaper in comparison with commonly used methods.

show abstract

“…Carbon materials, with their excellent performances such as high conductivity and strong absorbability, have been widely used as the matrix in biosensors and electrocatalysis [9] . In order to improve the stability at high temperature and the electrical conductivity of PANI, Liu et al [10] synthesized PANI-graphite oxide composite by mechanical mixing.…”

Section: Introductionmentioning

confidence: 99%

Polyaniline-graphite composite film glucose oxidase electrode

Zhou

Chen

et al. 2006

J Cent. South Univ. Technol.

View full text Add to dashboard Cite

A novel polyaniline-graphite composite film glucose oxidase (PGCF GOD) electrode was developed. The PGCF was synthesized by cyclic voltammetry method in 0.5 mol/L H 2 SO 4 solution containing 1 g/L graphite powder and 0.2 mol/L aniline. The PGCF GOD electrode was prepared by doping GOD into the composite film. The morphology of the PGCF and the response property of the PGCF GOD electrode were investigated by scanning electron microscopy and electrochemical measurement, respectively. The results show that the PGCF has a porous and netty structure and the PGCF GOD electrode has excellent response property such as high sensitivity and short response time. Influences of pH value, temperature, glucose concentration and potential on the response current of the electrode were also discussed. The sensor has a maximum steady-state current density of 357.17 µA/cm 2 and an apparent Michaelis-Menten constant of 16.57 mmol/L. The maximum current response of the enzyme electrode occurs under the condition of pH 5.5, 0.8 V and 65 ℃.

show abstract

Affinity biosensors based on disposable screen-printed electrodes modified with DNA

Cited by 26 publications

References 29 publications

Amperometric DNA quantification based on the use of peroxidase-mercaptopropionic acid-modified gold electrodes

Amperometric DNA quantification based on the use of peroxidase-mercaptopropionic acid-modified gold electrodes

Potentiometric DNA Sensor Based on Electropolymerized Phenothiazines for Protein Detection

Polyaniline-graphite composite film glucose oxidase electrode

Contact Info

Product

Resources

About