Affinitätschromatographie an stationär gebundenen oligonukleotiden

N-Anisoyl-deoxyribocytidine-5'-phosphate is chemically polycondensed. From the polycondensate, oligomers containing four or more monomeric units are isolated chromatographically and subsequently linked to a soluble polyvinylalcohol. After cleavage of the nucleobaseprotecting groups, the unreacted oligodesoxyribocytidylic acids are separated from polyvinylalcohol bound oligomers through ultrafiltration. DEAE-Cellulose is subsequently coated with this polymer-bound oligodeoxyribocytidylic acids. The now obtained PVAL-p(dC),-DEAECellulose acts as a specific sorbent in template-chromatography.

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Immobilisierung von Oligodesoxyriboguanylsäuren an vernetztem Polyvinylalkohol

Schott

Watzlawick

1981

Makromol. Chem.

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Protected homologs of deoxyriboguanylic acid are synthesized on a preparative scale by condensation of monomers according to the diester method. Oligomers with four and more monomeric units are isolated and bound to the free hydroxyl groups of an insoluble poly(vinylalcohol) gel via ester linkage with their 5'‐phosphate groups. The oligo(guanylic acid) gel, obtained after cleavage of the protective groups, is suitable for preparative template‐chromatography of nucleotide mixtures.

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Affinitätschromatographie an stationär gebundenen oligonukleotiden

Cited by 12 publications

References 9 publications

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Synthese von tryptophanpeptid‐gelen auf polyacrylbasis zur untersuchung der peptid‐nukleotid‐wechselwirkung

Immobilisierung von polymergebundenen oligodesoxyribocytidylsäuren an DEAE‐cellulose

Immobilisierung von Oligodesoxyriboguanylsäuren an vernetztem Polyvinylalkohol

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