Synthese von tryptophanpeptid‐gelen auf polyacrylbasis zur untersuchung der peptid‐nukleotid‐wechselwirkung

Eckstein, Heiner; Schott, Herbert; Bayer, Ernst

doi:10.1002/macp.1976.021770305

Makromol. Chem.

1976

DOI: 10.1002/macp.1976.021770305

|View full text |Cite

Synthese von tryptophanpeptid‐gelen auf polyacrylbasis zur untersuchung der peptid‐nukleotid‐wechselwirkung

Heiner Eckstein

Herbert Schott

Ernst Bayer

Abstract: EinleitunyDesoxyribooligonukleotide,die kovalent an Polyvinylalkohol gebunden sind, werden an DEAE-Cellulose') als Polyanionen unter bestimmten Bedingun-*I 2-Diathylaminoathyl-Cellulose.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1977

1981

Publication Types

Select...

Article3

Relationship

Self Cite2

Independent1

Authors

Journals

Cited by 3 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One method uses the fieldjump technique [14]. The other, a very simple chromatographic approach [15,16], allows one to determine directly the strength and specificity of the oligopeptide-oligonucleotide interactions from the retardation of dissolved oligonucleotides on immobilized amino acids or oligopeptides.Using this chromatographic procedure, we have investigated whether lysine or lysine peptides undergo specific interactions with definite mononucleotides and oligonucleotides. With the help of these model investigations we have investigated whether lysine residues, statistically incorporated in a protein molecule, or regions of a protein rich in lysine, can recognize distinct nucleotides of dA .…”

mentioning

confidence: 99%

Studies on Interactions between Immobilized Lysine Residues and Oligomers of Thymidylic and Deoxyadenylic Acids

Schott

Eckstein

1980

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

Two groups of crosslinked polyacrylic gels with immobilized lysine and lysine peptides (Lys)~ and ( L y~)~-P r o have been used as models for the chromatographic investigation of lysine-peptide -oligonucleotide interactions. One group carries carboxylic groups in addition to the peptide residues in the gel matrix; the other gel type contains no such carboxylic groups in the gel matrix.Nucleotides of the series (dT)2-5, p(dT)l -4, p(dT)l -4p, (dA)2 -5 , p(dA)1-5 and p(dA)1-4p were chromatographed on these gels under various conditions in an aqueous buffer. On the gels of the first group the nucleotides were retarded only slighly, the positive charges of the &-amino groups being compensated partially or totally by the negatively charged carboxyl groups of the polymer matrix. On the gels of the second group, however, the oligonucleotides underwent specific interactions. These interactions were based primarily upon electrostatic forces between the positively charged E-amino groups of the immobilized peptides and the negatively charged phosphate groups of the oligonucleotides.Our results indicate that, in addition to the electrostatic interaction, the conformation plays a crucial role. We explain the selectivity of the interaction with a conformation-fit mechanism. The origin of this mechanism, which creates specific interactions from unspecific forces, is discussed.The organization of DNA and protein in chromatin is at present largely unknown and there is intense research activity in this area [l]. In order to evaluate and interpret histone-DNA interactions for example, model studies have been pursued using synthetic polypeptides [2-61, synthetic histone fragments [7] or histone [8-131 all complexed with DNA. Analysis of these interactions indicates a preferential binding of polylysine and lysine-rich histone to dA . dT-rich DNA. It was also found that the main characteristics which govern the interaction with DNA are located in the very lysine-rich part of the histone molecules In order to obtain more information about the elements of specificity in histone-DNA interactions, simple model systemes are required which allow one to characterize the individual interactions separately. To investigate the binding specificity between a DNA and a histone region, it ought to be profitable to apply [8,121. Abbreviations. The abbreviations for the nucleotides follow CBN Recommendations, see Eur. J . Biochem. 15,203-208 (1970). Note that (dN), has no terminal phosphates, p(dN), only the 5'-termlnal phosphate, p(dN).p both 3' and 5'-terminal phosphates.oligonucleotide-oligopeptide models. Until now, such analyses have not been performed, because convenient methods for the characterization of oligonucleotideoligopeptide interactions have not been available.Recently two methods have been published which make it possible to investigate the oligonucleotideoligopeptide interactions. One method uses the fieldjump technique [14]. The other, a very simple chromatographic approach [15,16], allows one to determine directly the strength...

show abstract

mentioning

confidence: 99%

Studies on Interactions between Immobilized Lysine Residues and Oligomers of Thymidylic and Deoxyadenylic Acids

Schott

Eckstein

1980

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

Conformation affinity of cross-linked polyacrylic gels towards nucleobases, nucleosides and nucleotides

Eckstein

Schott

1977

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein

View full text Add to dashboard Cite

Synthesis of Histone Model Sequences for Immobilization on a Cross‐linked Polyacrylate Matrix

Eckstein

Bayer

1981

International Journal of Peptide and Protein Research

Self Cite

View full text Add to dashboard Cite

which can be used directly for radical copolymerization. [LysJs-Pro is synthesized according to the conventional peptide synthesis using the carbobenzoxy group for N"-protection and the tert.-butyl group for side chain protection. The carboxyl function is protected as tert.-butyl ester. The undecapeptide is synthesized in a similar manner. Instead of purifying the intermediate peptides by extraction, chromatography is used exclusively. The isolation o f the unprotected Pro and their characterization using amino acid analysis, electrophoreses and field desorption mass spectrometry is also reported.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Synthese von tryptophanpeptid‐gelen auf polyacrylbasis zur untersuchung der peptid‐nukleotid‐wechselwirkung

Abstract: EinleitunyDesoxyribooligonukleotide,die kovalent an Polyvinylalkohol gebunden sind, werden an DEAE-Cellulose') als Polyanionen unter bestimmten Bedingun-*I 2-Diathylaminoathyl-Cellulose.

Cited by 3 publications

References 12 publications

Studies on Interactions between Immobilized Lysine Residues and Oligomers of Thymidylic and Deoxyadenylic Acids

Studies on Interactions between Immobilized Lysine Residues and Oligomers of Thymidylic and Deoxyadenylic Acids

Conformation affinity of cross-linked polyacrylic gels towards nucleobases, nucleosides and nucleotides

Synthesis of Histone Model Sequences for Immobilization on a Cross‐linked Polyacrylate Matrix

Contact Info

Product

Resources

About