Oat containing rich β-glucan,
polyphenols, flavonoids, saponins,
alkaloids, and other substances shows good biological activities.
Therefore, the present study aimed to uncover the possible mechanism
and therapeutic effect of Avenanthramide C in lessening inflammatory
responses in pediatric pneumonia. Pediatric pneumonia was induced
by liposaccharide (LPS) for vivo model and vitro model. Macrophage
was performed to determine the mechanism and effects of Avenanthramide
C in pediatric pneumonia. NLRP3 activity participated in the effects
of Avenanthramide C in pediatric pneumonia. Avenanthramide C induced
p-PI3K and p-Akt expressions and reduced ubiquitination of PI3K expression
in pediatric pneumonia. On the other hand, Avenanthramide C integrated
serine at 821 sites of the PI3K protein function. Avenanthramide C
reduced ROS (reactive oxygen species)-induced mitochondrial damage
by PI3K/AKT function in a model of pediatric pneumonia. Avenanthramide
C protects pyroptosis in a model of pediatric pneumonia by PI3K/AKT/Nrf2/ROS
signaling. Taken together, our results demonstrated that Avenanthramide
C protects pyroptosis through dependent ROS-induced mitochondrial
damage by PI3K ubiquitination and phosphorylation in a model of pediatric
pneumonia, suggesting its potential use for the treatment of pediatric
pneumonia and other inflammatory diseases.