Avenanthramide C from Oats Protects Pyroptosis through Dependent ROS-Induced Mitochondrial Damage by PI3K Ubiquitination and Phosphorylation in Pediatric Pneumonia

Abstract

Oat containing rich β-glucan, polyphenols, flavonoids, saponins, alkaloids, and other substances shows good biological activities. Therefore, the present study aimed to uncover the possible mechanism and therapeutic effect of Avenanthramide C in lessening inflammatory responses in pediatric pneumonia. Pediatric pneumonia was induced by liposaccharide (LPS) for vivo model and vitro model. Macrophage was performed to determine the mechanism and effects of Avenanthramide C in pediatric pneumonia. NLRP3 activity participated in the effects of Avenanthramide C in pediatric pneumonia. Avenanthramide C induced p-PI3K and p-Akt expressions and reduced ubiquitination of PI3K expression in pediatric pneumonia. On the other hand, Avenanthramide C integrated serine at 821 sites of the PI3K protein function. Avenanthramide C reduced ROS (reactive oxygen species)-induced mitochondrial damage by PI3K/AKT function in a model of pediatric pneumonia. Avenanthramide C protects pyroptosis in a model of pediatric pneumonia by PI3K/AKT/Nrf2/ROS signaling. Taken together, our results demonstrated that Avenanthramide C protects pyroptosis through dependent ROS-induced mitochondrial damage by PI3K ubiquitination and phosphorylation in a model of pediatric pneumonia, suggesting its potential use for the treatment of pediatric pneumonia and other inflammatory diseases.

Latex derived from Ficus carica L. inhibited the growth of NSCLC by regulating the caspase/gasdermin/AKT signaling pathway

“…46 In clinical samples, various subtypes of AKT are frequently shown to be overexpressed and mutated. 47 AKT has been linked to pyroptosis and apoptosis in recent years, according to research 48,49 This study also showed that AFFL could reduce EGFR overexpression and inhibit AKT protein expression in H460 and A549 cells (Fig. 6A and B).…”

The effects and mechanisms of the anti-COVID-19 traditional Chinese medicine, Dehydroandrographolide from Andrographis paniculata (Burm.f.) Wall, on acute lung injury by the inhibition of NLRP3-mediated pyroptosis

“…All mice were randomly assigned to experimental groups and then anesthetized using 50 mg/kg pentobarbital sodium. Mice in the sham group (n = 6) were injected with normal saline, and mice in the ALI model group (n = 10) were injected with LPS (2 mg/kg, Sigma‒Aldrich) for 24 h according to the literature ( Pu et al, 2022 ). The lung injury score and W/D rate were measured according to the literature ( Pu et al, 2022 ).…”

“…Mice in the sham group (n = 6) were injected with normal saline, and mice in the ALI model group (n = 10) were injected with LPS (2 mg/kg, Sigma‒Aldrich) for 24 h according to the literature ( Pu et al, 2022 ). The lung injury score and W/D rate were measured according to the literature ( Pu et al, 2022 ). Mice in the Deh group (n = 10) received 12.5, 25, or 50 mg/kg Deh ( SMB00350 , ≥98% , Sigma‒Aldrich) or 1 mg/kg dexamethasone (Dex, D1756, ≥98% , Sigma‒Aldrich) for 24 h and then induced into the ALI model and 12.5, 25, or 50 mg/kg Deh for 24 h.…”

“…Murine bone marrow-derived macrophage (BMDM) cells were extracted and induced into an in vitro model of ALI as described in the literature ( Pu et al, 2022 ). BMDMs were isolated from C57BL/6 mice and induced with 30% L929 cell-conditioned medium and 20% FBS for 1 week.…”

6'-O-galloylpaeoniflorin alleviates inflammation and oxidative stress in pediatric pneumonia through activating Nrf2 activation

“…Lipopolysaccharide is the prime component of endotoxin and is considered as the most important pathogen resulting in lung inflammation and sepsis. [13][14][15] LPS activates a large number of inflammatory cells and induces pneumonia through a variety of signaling pathways. [16][17][18] In this study, we successfully constructed a pneumonia model using LPS, and confirmed the effects of GPF on this model.…”