Aeromonas veronii caused bilateral exophthalmia and mass mortality in cultured Nile tilapia, Oreochromis niloticus (L.) in India

Raj, N. Sundar; Swaminathan, T. Raja; Dharmaratnam, D. Arathi; Raja, S. Arun; Ramraj, D.; Lal, Kuldeep Kumar

doi:10.1016/j.aquaculture.2019.734278

Cited by 72 publications

(62 citation statements)

References 30 publications

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“…Bacterial diseases are one of the most limiting factors in freshwater aquaculture (Raj et al., 2019). As an emerging global opportunistic pathogen, S. maltophilia has not only been found in humans but also in aquatic animals such as fish and turtles (Cao et al., 2017; Geng et al., 2010; Martinez‐Silvestre et al., 2017).…”

Section: Discussionmentioning

confidence: 99%

Emerging pathogens caused disease and mortality in freshwater mussels,Hyriopsis cumingii,in China

Yang

et al. 2020

Aquac Res

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In August 2019, massive mortalities of cultured Hyriopsis cumingii occurred in a local shellfish breeding experimental base in Chongqing, China. In this report, we identified 14 isolates from cultured mussels in mortality events and demonstrated that these strains caused mortality from 9% to 97%. Significant mussel mortality (p < .05) was observed in association with most isolates after bacterial challenge; however, considerable variation in the virulence of different isolates was noted, with survival ranging from 3% to 88% after 7 days. The most virulent strains (HOP3 and GL1) in haemolymph and tissues were identified as Stenotrophomonas maltophilia and Aeromonas veronii based on biochemical and 16S rDNA sequence analysis. The LD50 values of HOP3 and GL1 were found to be 2.45 × 106 CFU/g (body weight) and 2.24 × 105 CFU/g (body weight) respectively. Histopathology of H. cumingii infected with S. maltophilia and A. veronii displayed degeneration of muscle fibres and gill necrosis. We tested the antibiotic susceptibility of the two isolates and found that HOP3 was multidrug‐resistant. Growth optimization study revealed that both isolates remained surviving across wide environmental ranges. Our data lay the foundation for the pathogenic mechanism of mussel bacterial diseases, and they also suggest that effective control measures are worth further investigation.

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Section: Discussionmentioning

confidence: 99%

Emerging pathogens caused disease and mortality in freshwater mussels,Hyriopsis cumingii,in China

Yang

et al. 2020

Aquac Res

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“…It is commonly isolated from environmental, clinical, and food samples (Ghenghesh et al, 2008). A. veronii strains have increasing been isolated from diseased fish (Hoai et al, 2019;Raj et al, 2019). The clinical symptoms of infected fish commonly comprised ulcer, fin rot/tail rot, abdominal distention, exophthalmia and hemorrhage.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification and Characteristics of Aeromonas veronii From Diseased Crucian Carp (Carassius auratus gibelio)

et al. 2019

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Aeromonas species often cause disease in farmed fish. In the present study, dominant bacteria were isolated from diseased crucian carp (Carassius auratus gibelio). Based on this, a bacterial isolate was tentatively named CFJY-623. This isolate was identified as Aeromonas veronii based on analysis of its morphological, physiological, and biochemical features, as well as 16S rRNA and gyrB gene sequences. Six virulence genes related to pathogenicity including aerolysin, cytotonic enterotoxins, elastase, glycerophospholipid: cholesterol acyltransferase, lipase, and serine protease were identified in this A. veronii isolate. The median lethal dosage (LD50) of the CFJY-623 isolate for crucian carp was determined as 1.31 × 10 7 CFU/mL. Artificial experimental infection showed that the CFJY-623 isolate caused considerable histological lesions in the fish, including tissue cell degeneration, necrosis, and inflammatory cell infiltrating. Drug sensitivity testing showed that the isolate was susceptible to aminoglycosides, carbapenemes, and nitrofurans. Exploring its growing features showed that this isolate exhibited a high level of environmental adaptability. These results provided a scientific basis for the identification of A. veronii and treatment for fish infected by this pathogen.

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“…35(2), e14442. July-December, 2021 Doi: https://dx.doi.org/10.15359/ru.35-2.4 URL: https://www.revistas.una.ac.cr/uniciencia E-ISSN: 2215-3470 Email: revistauniciencia@una.cr CC: BY-NC-ND 12 con un alto potencial para causar un impacto negativo en los cultivos de tilapia a nivel nacional, ya que son bacterias oportunistas e inmunosupresores (Raj et al, 2019). En países como Malasia, Egipto y Pakistán se reporta el aislamiento de las mismas bacterias en sistemas de producción de tilapia, lo cual provoca impactos negativos (Dong et al, 2017;Li & Cai, 2011;Shayo et al, 2012).…”

Section: Discussionunclassified

Patógenos bacterianos y su resistencia a los antimicrobianos en los cultivos de tilapia en Guatemala

García-Pérez

Ulloa-Rojas²,

Mendoza-Elvira

2021

Uniciencia

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Este estudio muestra el trabajo realizado durante el año 2018-2019, donde se identificaron los principales patógenos bacterianos que afectan los cultivos de la tilapia en Guatemala, al mismo tiempo se evaluó la resistencia a los antibióticos de mayor uso en la industria acuícola de esta zona. Se recolectaron peces con peso de 50 a 1 000 g, en diversos centros de producción. Por cada organismo se registró la signología macroscópica externa e interna más frecuente, posteriormente se aislaron muestras bacteriológicas de fluido sanguíneo, úlceras y otras áreas hemorrágicas, riñón anterior, bazo, meninges, hígado y corazón. Las bacterias patógenas aisladas fueron identificadas a nivel de especie, a través de pruebas bioquímicas. Finalmente se evaluó la susceptibilidad con los antibióticos: oxitetraciclina (40 µg), florfenicol (40 µg), enrofloxacina (40 µg) y fosfomicina (40 µg) por el método de difusión de disco. Los organismos evidenciaron, en su mayoría, ulceras cutáneas, áreas hiperémicas, melanización corporal, hígado pálido y friable, vasculitis en hígado, congestión y hemorragia intestinal. Se lograron aislar 16 cepas bacterianas patógenas para la tilapia, los géneros más frecuentes fueron: Aeromonas (70 %: 3 spp), seguido por Plesiomonas, Burkholderia, Pseudomonas, Shewanella y Streptococcus (6 %: 1 sp). Se observó que las cepas bacterianas aisladas mostraron resistencia hacia los antibióticos oxitetraciclina (25 %) y fosfomicina (25 %). Todas las cepas bacterianas aisladas evidenciaron sensibilidad a florfenicol y enrofloxacina. La determinación de los patógenos más frecuentes, así como su resistencia antimicrobiana en la industria acuícola, es el primer reporte que se genera para Guatemala.

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Aeromonas veronii caused bilateral exophthalmia and mass mortality in cultured Nile tilapia, Oreochromis niloticus (L.) in India

Cited by 72 publications

References 30 publications

Emerging pathogens caused disease and mortality in freshwater mussels,Hyriopsis cumingii,in China

Emerging pathogens caused disease and mortality in freshwater mussels,Hyriopsis cumingii,in China

Isolation, Identification and Characteristics of Aeromonas veronii From Diseased Crucian Carp (Carassius auratus gibelio)

Patógenos bacterianos y su resistencia a los antimicrobianos en los cultivos de tilapia en Guatemala

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