Aeromonas simiae sp. nov., isolated from monkey faeces

Harf-Monteil, Colette; Flèche, Anne Le; Riegel, Philippe; Prévost, Gilles; Bermond, Delphine; Grimont, Patrick A. D.; Monteil, H.

doi:10.1099/ijs.0.02786-0

Cited by 80 publications

(34 citation statements)

References 27 publications

(29 reference statements)

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“…For most housekeeping genes studied, intraspecies nucleotide substitution rates are Ͻ2% (different strains within the same species), while interspecies values (strains belonging to different species) are typically Ͼ3%. Using these numbers as baseline values, one can determine which groups of species do not fall within these parameters ( (109,301). Such outliers may simply be a reflection of the methodology used, gene sequenced, or strains analyzed.…”

Section: Aeromonas Classificationmentioning

confidence: 99%

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

2010

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SUMMARY Over the past decade, the genus Aeromonas has undergone a number of significant changes of practical importance to clinical microbiologists and scientists alike. In parallel with the molecular revolution in microbiology, several new species have been identified on a phylogenetic basis, and the genome of the type species, A. hydrophila ATCC 7966, has been sequenced. In addition to established disease associations, Aeromonas has been shown to be a significant cause of infections associated with natural disasters (hurricanes, tsunamis, and earthquakes) and has been linked to emerging or new illnesses, including near-drowning events, prostatitis, and hemolytic-uremic syndrome. Despite these achievements, issues still remain regarding the role that Aeromonas plays in bacterial gastroenteritis, the extent to which species identification should be attempted in the clinical laboratory, and laboratory reporting of test results from contaminated body sites containing aeromonads. This article provides an extensive review of these topics, in addition to others, such as taxonomic issues, microbial pathogenicity, and antimicrobial resistance markers.

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Section: Aeromonas Classificationmentioning

confidence: 99%

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

2010

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“…To our 39 knowledge, since its description (Harf- Monteil et al, 2004), no more isolates of this species have 40 been identified. Further studies showed identical gyrB, rpoD and 16S rDNA sequences (Saavedra 41 et al, 2006) and the same genotyping pattern , revealing that they are two 42…”

Section: Introduction 25mentioning

confidence: 99%

Phylogenetic identification of Aeromonas simiae from a pig, first isolate since species description

Fontes¹,

Saavedra²,

Monera³

et al. 2010

Veterinary Microbiology

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Please cite this article as: Fontes, M.C., Saavedra, M.J., Monera, A., Martins, C., Martínez-Murcia, A., Phylogenetic identification of Aeromonas simiae from a pig, first isolate since species description, Veterinary Microbiology (2008Microbiology ( ), doi:10.1016Microbiology ( /j.vetmic.2009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. The species Aeromonas simiae was first described on the basis of two strains (CIP 12 T and CIP 107797) isolated from faeces of healthy monkeys (M. fascicularis) from 13Mauritius, which were kept in quarantine in the Centre for Primatology, Louis Pasteur University, 14 Strasbourg, France. In the present study, during a survey to determine the prevalence of 15Aeromonas at three different pig slaughterhouses at north of Portugal, a single strain MDC2374 16 (out of 703 isolates) was identified as Aeromonas simiae on the basis of 16S rDNA, gyrB and 17 rpoD sequencing, confirming that traditional biochemical approaches are not resolute to identify 18 rarely isolated Aeromonas species. To our knowledge, this is the first time since species 19 description that A. simiae is newly isolated and identified. . 20 21

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“…At the time of writing, the genus Aeromonas includes 20 recognized species: Aeromonas hydrophila (the type species), A. bestiarum, A. salmonicida, A. caviae, A. media, A. eucrenophila, A. sobria, A. veronii, A. jandaei, A. schubertii, A. trota, A. allosaccharophila, A. encheleia, A. popoffii (Martin-Carnahan & Joseph, 2005), A. simiae (Harf-Monteil et al, 2004), A. molluscorum (Miñana-Galbis et al, 2004), A. bivalvium (Miñana-Galbis et al, 2007), A. aquariorum , A. tecta and the recently described A. fluvialis, isolated from river water (Alperi et al, 2010). Furthermore, Aeromonas piscicola, isolated from diseased fish, has been proposed by our group (Beaz-Hidalgo et al, 2009), as well as some reclassifications (Martínez-Murcia et al, 2007).…”

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confidence: 99%

Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., clinical species from Taiwan

Alperí

Martínez‐Murcia

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Two clinical Aeromonas strains (A2-50 T and A2-67 T ) recovered from the wounds of two patients in Taiwan could not be assigned to any known species of this genus based on their 16S rRNA gene sequences, which showed similarities of 99.6-99.8 % to those of the type strains of Aeromonas caviae, A. trota and A. aquariorum. The rpoD phylogenetic tree allocated these strains to two novel and independent phylogenetic lines, the neighbouring species being A. caviae, the type strain of which showed 93.2 % similarity (56 bp differences) to strain A2-50 T and 92.2 % (63 bp differences) to strain A2-67 T . A multilocus phylogenetic analysis of five housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 3684 bp) confirmed that the two strains formed independent phylogenetic lineages within the genus. These data, together with phenotypic characterization and DNA-DNA reassociation results, revealed that these strains represent novel Aeromonas species, for which the names Aeromonas taiwanensis sp. nov. (type strain A2-50 T 5CECT 7403 T 5LMG 24683 T ) and Aeromonas sanarellii sp. nov. (type strain A2-67 T 5CECT 7402 T 5LMG 24682 T ) are proposed.The genus Aeromonas includes facultatively anaerobic, Gramnegative, non-spore-forming bacilli or coccobacilli that are generally motile, usually oxidase-and catalase-positive, able to reduce nitrate to nitrite and generally resistant to the vibriostatic agent O/129 (2,4-diamino-6,7-diisopropylpteridine) (Abbott et al., 2003;Martin-Carnahan & Joseph, 2005). The genus belongs to the family Aeromonadaceae, order Aeromonadales, class Gammaproteobacteria The taxonomy of this genus is complex because, despite each species apparently having specific phenotypic characteristics, biochemical identification is laborious and very imprecise, showing poor correlation with genotypic identification (Borrell et al., 1998;Soler et al., 2003;Figueras, 2005;Ormen et al., 2005). The 16S rRNA gene sequence similarity among species of the genus is very high, ranging from 96.7 to 100 % (Martínez-Murcia et al., 1992Saavedra et al., 2006). Furthermore, this gene presents microheterogeneities Martínez-Murcia et al., 2005;Morandi et al., 2005;Alperi et al., 2008) that reduce its usefulness for identification (Alperi et al., 2008); such was the case for Aeromonas culicicola Alperi et al., 2008), now considered a synonym of A. veronii (Huys et al., 2005). However, several housekeeping genes have proven to have Abbreviation: MLPA, multilocus phylogenetic analysis.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, gyrB, rpoD, recA, dnaJ and gyrA gene sequences of strains A2-50 T and A2-67 T are respectively FJ230077 and FJ230076, FJ807272 and FJ807277, FJ472928 and FJ472929, FJ472930 and FJ47931, FJ807270 and FJ807279 and FJ807274 and FJ807276. An unrooted 16S rRNA gene sequence-based maximum-parsimony phylogenetic tree, an unrooted MLPA minimum-evolution tree, details of differences in the 16S rRNA gene sequences of the novel strains and DNA-DNA hybridization results are available as supplementary material ...

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Aeromonas simiae sp. nov., isolated from monkey faeces

Cited by 80 publications

References 27 publications

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

Phylogenetic identification of Aeromonas simiae from a pig, first isolate since species description

Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., clinical species from Taiwan

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