Aeromonas salmonicida ssp. salmonicida lacking pigment production, isolated from farmed salmonids in Finland

Wıklund, Tom; Lönnström, L.-G.; Niiranen, H.

doi:10.3354/dao015219

Cited by 18 publications

(11 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, a pigment producing strain appeared to be intermediate in virulence. Wiklund, Lönnström & Niiranen (1993) reported a very slow pigment‐producing A. salmonicida ssp. salmonicida isolate to be highly virulent in rainbow trout, Oncorhynchus mykiss (Walbaum), subsequent to i.p.…”

Section: Discussionmentioning

confidence: 99%

“…However, as stated by Wiklund & Dalsgaard (1998), an atypical strain can be defined only as a strain which does not fit into the existing classification of A. salmonicida ssp. salmonicida , although studies have been published ascribing isolates showing atypical or aberrant biochemical characteristics, such as late‐pigmentation ( Wiklund et al . 1993 ), as typical strains.…”

Section: Discussionmentioning

confidence: 99%

“… Wiklund, T., Lönnström, L. & Niiranen, H. 1993 Aeromonas salmonicida ssp. salmonicida lacking pigment production, isolated from farmed salmonids in Finland.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Non‐pigment‐producing isolates of Aeromonas salmonicida subspecies salmonicida: isolation, identification, transmission and pathogenicity in Atlantic salmon, Salmo salar L.

Koppang¹,

Fjølstad

Melgård³

et al. 2000

Journal of Fish Diseases

View full text Add to dashboard Cite

Four non‐pigment‐producing isolates and two pigment‐producing isolates of Aeromonas salmonicida sp. salmonicida were isolated from the head‐kidney of diseased farmed Atlantic salmon, Salmo salar L. The cultural, morphological and biochemical features of the isolates were compared with those of reference strains. Injection and cohabitation experiments were performed. The only difference between the non‐pigment‐producing isolates and the pigment producing reference strains of A. salmonicida ssp. salmonicida was the inability of the former to produce pigment. In the injection experiments, the investigated non‐pigment‐producing isolate produced a significantly higher mortality compared with the mortality caused by the reference strain, whereas no difference in mortality was detected in the cohabitation experiments.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Non‐pigment‐producing isolates of Aeromonas salmonicida subspecies salmonicida: isolation, identification, transmission and pathogenicity in Atlantic salmon, Salmo salar L.

Koppang¹,

Fjølstad

Melgård³

et al. 2000

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

“…Two A. salmonicida subsp. salmonicida reference strains were included: a non‐pigment producing strain P110A/91 from brown trout, Salmo trutta m. lacustris L. (Wiklund, Lönnström & Niiranen 1993) and an A‐layer positive A. salmonicida subsp. salmonicida strain MT423 (Hirst & Ellis 1994).…”

Section: Bacterial Isolatesmentioning

confidence: 99%

Putative virulence factors of atypical Aeromonas salmonicida isolated from Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.)

Madetoja

Pylkkö²,

Pohjanvirta³

et al. 2003

Journal of Fish Diseases

View full text Add to dashboard Cite

Atypical Aeromonas salmonicida (AAS) causes generalized lethal infections in farmed Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.), and is thus a serious threat for culture of these fish species. Virulence factors were studied among isolates of AAS from Arctic charr (n = 20), European grayling (n = 19) and other fish species (n = 20), of which 48 were of Finnish and 11 of Swedish origin. All isolates produced an A-layer. Extracellular products (ECP) of the AAS isolates did not produce detectable gelatinase and caseinase activity in test assays. Analysis of the same ECP preparations with substrate sodium dodecyl sulphate-polyacrylamide gel electrophoresis showed weak proteolytic activity, indicating the different sensitivity of the detection methods used. The ECP from AAS isolates showed low cytotoxic activity against cultured cells. However, the ECP did not induce mortality in challenged Arctic charr. The results suggest that toxic components, like ECP, secreted by the bacterium may not be the major virulence factor in AAS-infection in Arctic charr and European grayling, and hence the pathogenesis also differs from the pathogenesis of AAS-infection in Atlantic salmon, Salmo salar L.

show abstract

“…Identification of a microbial pathogens isolate usually follows a series of morphological, biochemical, immunological, and molecular techniques. For the identification of A. salmonicida and Y. ruckeri from affected fish farm, different morphological and biochemical test are practiced in the world wide (Stevenson and Daly, 1982;Wiklund et al, 1993;Gudmundsdottir, 1998;Dalsgaard and Madsen, 2000;Welch et al, 2011). The accurate identification of bacteria would help to take proper steps to control the disease before massive economical loss.…”

Section: Introductionmentioning

confidence: 99%

Morphological and biochemical characterization of two fish pathogenic bacteria: Aeromonas salmonicida and Yersinia ruckeri for rapid diagnosis of fish disease

Akter

2017

Asian J. Med. Biol. Res

View full text Add to dashboard Cite

Aeromonas salmonicida and Yersinia ruckeri are two common pathogenic fish bacteria responsible for furunculosis and Enteric Red Mouth disease (ERM), respectively. For the characterization of these two pathogens, a series of morphological (pigmentation, hemolyse, motility and body shape), biochemical tests (Gram staining, catalase, oxidase and API 20E strips) and a soft ionization technique (MALDI-TOF/MS) were performed in the laboratory. Pigmentation, motility, hemolysis and body shape was used as a preliminary identification of the bacteria. Both of the species were identified with the entire biochemical test without any doubt except API20E strips test. Although the API profile of A. salmonicida (0006104) was identified with high confidence (99.6%), but Y. ruckeri was misidentified as Vibrio mimicus. There are strong supports against Vibrio mimicus as it is a human pathogen, grow at a temperature more than 20 o C, motile and oxidase positive bacteria. The Y. ruckeri is a non-motile fish bacteria and oxidase negative which are consistent with the study results. Agglutination test with Bionor Mono kit was also identified the bacteria as Y. ruckeri. For rapid diagnosis of infectious disease, accurate identification of pathogen is very important for commercial aquaculture.

show abstract

Aeromonas salmonicida ssp. salmonicida lacking pigment production, isolated from farmed salmonids in Finland

Cited by 18 publications

References 6 publications

Non‐pigment‐producing isolates of Aeromonas salmonicida subspecies salmonicida: isolation, identification, transmission and pathogenicity in Atlantic salmon, Salmo salar L.

Non‐pigment‐producing isolates of Aeromonas salmonicida subspecies salmonicida: isolation, identification, transmission and pathogenicity in Atlantic salmon, Salmo salar L.

Putative virulence factors of atypical Aeromonas salmonicida isolated from Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.)

Morphological and biochemical characterization of two fish pathogenic bacteria: Aeromonas salmonicida and Yersinia ruckeri for rapid diagnosis of fish disease

Contact Info

Product

Resources

About