Aerobic, phenol-induced TCE degradation in completely mixed, continuous-culture reactors

Abstract

Both Pseudomonas putida F1 and a mixed culture were used to study TCE degradation in continuous culture under aerobic, non-methanotrophic conditions. TCE mass balance studies were performed with continuous culture reactors to determine the total percent removed in the reactors, and to quantify the percent removed by air stripping and biodegradation. Adsorption of TCE to biomass was assumed to be negligible. This research demonstrated the feasibility of treating TCE-contaminated water under aerobic, non-methanotrophic conditions with a mixed-culture, continuous-flow system. Initially glucose and acetate were fed as primary substrates. Pnenol, which has been shown to induce TCE-degrading enzymes, was fed at a much lower concentration (20 mg/L). Little degradation of TCE was observed when acetate and glucose were the primary substrates. After omitting glucose and acetate from the feed and increasing the phenol concentration to 50 mg/L, TCE biotransformation was observed at a significant level (46%). When the phenol concentration in the feed was increased to 420 mg/L, 85% of the incoming TCE was estimated to have been biodegraded. Under the same conditions, phenol utilization by the mixed culture was greater than that of P. putida F1, and TCE degradation by the mixed culture (85%) exceeded that of P. putida F1 (55%). The estimated percent-of-TCE biodegraded by the mixed culture was consistently greater than 80% when phenol was fed at 420 mg/L. Biodegradation of TCE was also observed in mixed-culture, batch experiments.

Construction and Use of an ipb DNA Module To Generate Pseudomonas Strains with Constitutive Trichloroethene and Isopropylbenzene Oxidation Activity

“…There are at present no hints of a certain physiological reaction which may be involved in signal transduction of putative catabolic repression of the ipb operon in Pseudomonas sp. strain JR1, but analogous repressing effects of easily metabolizable substrates on TCE cooxidation activities have already been reported (7,11,14,22).…”

The effect of COVID-19 derived cytokine storm on cancer cells progression: double-edged sword

“… Daunorubicin IL-3 IL-3 can induce ERK pathway. ERK/Mcl-1 pathway can inhibit BCL2 protein activity, and lead to drug resistance [ 94 ] Cyclophosphamide IL-6 IL6 can induce drug resistance via JAK/STAT3 and NF-kappaB IL6-dependent pathway [ 95 ] Taxotere IL-8 IL-8 can induce drug resistance and cancer progression, through PLC-PKC, PI3K-AKT, Rho-GTPase family, FAK/Src and MAPK cascade signals and NF-κB pathway [ 96 ] Methyl methane sulfonate TGF-β TGF-β can induce drug resistance through miR21 expression and subsequent suppression of MSH2 mRNA production [ 97 ] 5-Fluorouracil OSM* OSMRs/JAK1/STAT3 axis contributes to the resistance to the targeted drugs in cancer cells [ 95 , 98 ] Methotrexate IL-17 IL-17 leads to the production of KEAP1-Nrf2 and NF-KB transcription factors, through PI3K/AKT/mTOR pathway; it can induce drug resistance by expressing the BCRP molecule [ 99 , 100 ] Etoposide IL-11 IL-11 induces resistance to chemotherapy drugs, through gp130/JAK/STAT3/Bcl2 pathway [ 101 ] Oxaliplatin CXCL1 Drug resistance is induced via CXC-Chemokine/NF-κB signaling pathway [ 102 ] Abbreviation: IL interleukin, ERK extracellular signal-regulated kinase, Mcl-1 myeloid cell leukemia 1, Bax Bcl-2-associated X protein, JAK Janus kinase, STAT signal transducer and activator of transcription 3, NF-Kβ nuclear factor-kβ, PLC phospholipase C, PKC protein kinase C, PI3K phosphoinositide 3-kinase, Akt serine threonine kinase, ...…”

Modeling trichloroethylene degradation by a recombinant pseudomonad expressing toluene ortho‐monooxygenase in a fixed‐film bioreactor

“…However, most of these pollutants can be utilized by the bacteria as the sole carbon and energy source for growth. Some volatile chlorinated aliphatic compounds can only be degraded through a cometabolic pathway, and trichloroethylene (TCE) has been used as the model pollutant (Coyle et al, 1993;Folsom and Chapman, 1991;Lackey et al, 1993;Phelps et al, 1990;Taylor et al, 1993;Wilcox et al, 1995). The most commonly studied microorganisms for TCE degradation are Methylosinus trichosporium OB3b (McFarland et al, 1992;Oldenhuis et al, 1989;Strand et al, 1991) and Burkholderia cepacia G4 (formerly Pseudomonas cepacia G4) (Ensley and Kurisko, 1994;Folsom and Chapman, 1991;Landa et al, 1994;Nelson et al, 1986;Shields et al, 1991).…”