Aerobic Exercise Reduces Asthma Phenotype by Modulation of the Leukotriene Pathway

Alberca, Ricardo Wesley; Greiffo, Flavia R.; MacKenzie, BreAnne; Oliveira-Júnior, Manoel Carneiro; Andrade-Sousa, Adilson Santos; Graudenz, Gustavo Silveira; Santos, Ângela Batista Gomes dos; Damaceno-Rodrigues, Nilsa Regina; Castro‐Faria‐Neto, Hugo C.; Arantes-Costa, Fernanda Magalhães; Martins, Mílton de Arruda; Abbasi, Asghar; Lin, Chin Jia; Idzko, Marco; Oliveira, Ana Paula Ligeiro de; Northoff, Hinnak; Vieira, Rodolfo Paula

doi:10.3389/fimmu.2016.00237

Cited by 28 publications

(24 citation statements)

References 33 publications

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“…IHC images (5 airways and 15 parenchymal fields) from each slide of each mouse from all experimental groups were taken using an Olympus BX40 microscope at ×400 magnification and CellSens software. The percent epithelial area positive for IL-10 and NF-κB as well as the number of positive cells per square millimeter of parenchymal tissue were presented as previously described [17-19]. …”

Section: Methodsmentioning

confidence: 99%

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

et al. 2018

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Background: Pseudomonas aeruginosa (PS) infection results in severe morbidity and mortality, especially in immune-deficient populations. Aerobic exercise (AE) modulates the immune system, but its effects on the outcomes of pulmonary PS infection in elderly mice are unknown. Methods: BALB/c mice (24 weeks old) were randomized to sedentary, exercise (EX), PS, and PS + EX groups for the acute experimental setting, and PS and PS + EX groups for the chronic setting. Low-intensity AE was performed for 5 weeks, 60 min/day; 24 h after the final AE session, mice were inoculated with 5 × 10⁴ colony-forming units (CFU) of PS, and 24 h and 14 days after PS inoculation, mice were studied. Results: AE inhibited PS colonization (p < 0.001) and lung inflammation (total cells, neutrophils, lymphocytes [p < 0.01] in bronchoalveolar lavage [BAL]), with significant differences in BAL levels of IL-1β (p < 0.001), IL-6 (p < 0.01), CXCL1 (p < 0.001), and TNF-α (p < 0.001), as well as parenchymal neutrophils (p < 0.001). AE increased BAL levels of IL-10 and parenchymal (p < 0.001) and epithelial (p < 0.001) IL-10 expression, while epithelial (p < 0.001) and parenchymal (p < 0.001) NF-κB expression was decreased. AE diminished pulmonary lipid peroxidation (p < 0.001) and increased glutathione peroxidase (p < 0.01). Pre-incubation of BEAS-2B with IL-10 inhibited PS-induced epithelial cell expression of TNF-α (p < 0.05), CD40 (p < 0.01), and dichlorodihydrofluorescein diacetate (p < 0.05). Conclusions: AE inhibits PS-induced lung inflammation and bacterial colonization in elderly mice, involving IL-10/NF-κB, and redox signaling.

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Section: Methodsmentioning

confidence: 99%

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

et al. 2018

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“…It was demonstrated that aerobic exercise reduces nuclear factor kappa light-chain enhancer of activated B cells (NF-κB) activation and increases release of the anti-inflammatory cytokine interleukin (IL)-10 [ 70 ]. Low- to moderate-intensity aerobic exercise was found to reduce asthmatic inflammation in clinical and experimental models [ 71 ].…”

Section: Other Potential Measures To Improve Qol In Severe Asthmamentioning

confidence: 99%

Severe asthma and quality of life

Hossny

Caraballo

Casale

et al. 2017

World Allergy Organization Journal

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Severe asthma has a great impact on the quality of life (QOL) of patients and their families. The magnitude of this morbidity is affected by several personal factors including age. Appropriate asthma control and modifications of social roles and activities are expected to improve QOL. Biologics, primarily monoclonal antibodies, have been developed to target specific pathways and molecules important in the pathogenesis of asthma. The use of biologics has shown some promising effects on the QOL of patients with severe recalcitrant asthma. Other potential measures involve targeting risk factors and comorbidities and improving the levels of adherence to therapy. This article briefly reviews the impact of severe asthma on QOL and the potential methods to combat this morbidity including the available therapeutic biologics.

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“…Is it widely accepted that exercise training can ameliorate several pulmonary inflammatory symptoms [ 13 – 15 ], in a general way, due to the prominent anti-inflammatory effects associated. For instance, previous investigations on asthma models have identified that aerobic exercise training reduces the activity of leukotrienes and their mediators [ 22 ]. Leukotrienes are proactive inflammatory bioactive molecules, derived from the inflammatory lipoxygenase pathway, that act on the respiratory system as bronchoconstrictor, in the formation of edema, in mucus hypersecretion, and in the proliferation, activation, and survival of inflammatory cells [ 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Exercise Improves Lung Inflammation, but Not Lung Remodeling and Mechanics in a Model of Bleomycin-Induced Lung Fibrosis

El-Mafarjeh

Martins

Probst

et al. 2020

Oxidative Medicine and Cellular Longevity

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Introduction. Moderate aerobic exercise training accelerates the resolution of lung fibrosis in a model of bleomycin-induced pulmonary fibrosis. However, whether it can inhibit the development of lung fibrosis is unknown. Materials and Methods. C57Bl/6 mice were distributed into four groups: Control (Co), Exercise (Exe), Bleomycin (Bleo), and Bleomycin+Exercise (Bleo+Exe). A single bleomycin dose (1.5 UI/kg) was administered orotracheally and treadmill exercise started in the same day, enduring for 4 weeks, 5x/week, 60 minutes/session, at moderate intensity. Lung mechanics, systemic and pulmonary inflammation, and lung remodeling were evaluated. Lung homogenates were used to evaluate the antioxidant status. Results. Total cells, macrophages, lymphocytes, and neutrophils numbers, in agreement with IL-6 levels, were higher in the BAL and serum of Bleo group, compared to other groups. In addition, lung levels of LTB4 in Bleo were higher than other groups, whereas SOD activity and nitric oxide levels in exercised groups (Exe and Exe+Bleo) compared to the Bleo group. Lung GPX activity was lower in Bleo and Exe+Bleo groups compared to others. Exe and Exe+Bleo groups also showed higher IL-10 expression by lung macrophages than other groups, whereas TGF-β expression was higher in Exe, Bleo, and Exe+Bleo groups compared to control. CCR7 expression was induced only in the Exe group. However, exercise did not improve lung remodeling and mechanics, or serum and pulmonary levels of VEGF, IGF-1, and TGF-β. Conclusion. Aerobic exercise training initiated concomitantly with induction of pulmonary fibrosis reduces lung and systemic inflammation but fails to inhibit lung fibrosis and mechanics impairment.

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Aerobic Exercise Reduces Asthma Phenotype by Modulation of the Leukotriene Pathway

Cited by 28 publications

References 33 publications

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

Severe asthma and quality of life

Exercise Improves Lung Inflammation, but Not Lung Remodeling and Mechanics in a Model of Bleomycin-Induced Lung Fibrosis

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