Aerobic condition increases carotenoid production associated with oxidative stress tolerance in<i>Enterococcus gilvus</i>

Hagi, Tatsuro; Kobayashi, Miho; Nomura, Masaru

doi:10.1111/1574-6968.12341

Cited by 16 publications

(10 citation statements)

References 25 publications

(49 reference statements)

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“…Thereafter, the five Lactobacillus isolates were cultured in de Man, Rogosa and Sharpe (MRS) media for 24 h at 30°C, followed by extracting the yellow pigments that were finally isolated [22]. Consistent with these findings, various LAB, including E. gilvus, L. plantarum, L. fermentum, Pediococcus acidilactici, and P. pentosaceus, have been shown to produce deep yellow pigmentation, which could be involved in C 3 0 carotenoid compounds, in previous studies [10,12,13,15,21].…”

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confidence: 52%

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Isolation of Lactobacillus plantarum subsp. plantarum Producing C30 Carotenoid 4,4��-Diaponeurosporene and the Assessment of Its Antioxidant Activity

Kim

Seo

Cha

et al. 2019

Journal of Microbiology and Biotechnology

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Carotenoids are natural pigments widespread in plants, some animals, fungi, and photosynthetic or nonphotosynthetic bacteria [1]. Their antioxidant activities have also been extensively studied [2]. Carotenoids have interesting biological functions including antioxidant, anticancer, and anti-obesity effects and are widely used in foods, nutraceuticals, and the feed industry [3]. Structurally, carotenoids can be divided into carotenes (e.g., α-/βcarotene and lycopene) and xanthophylls (e.g., lutein, zeaxanthin, fucoxanthin, and astaxanthin) [3]. Several types of carotenoids are produced by microorganisms. The yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) produces astaxanthin [4], and microalgae produce lutein [5]. The fungus Blakeslea trispora has been reported to produce β-carotene and lycopene [6]. Lactic acid bacteria (LAB) are a type of gram-positive bacteria that are generally recognized as safe (GRAS) and have had diverse applications in fermentation of various foods for many centuries [7, 8]. LAB exhibit microaerophilic or facultative anaerobic growth characteristics owing to their lack of catalase expression and inability to remove free radicals [8]. To overcome this problem, LAB produce various antioxidants, such as superoxide dismutase and thioredoxin reductase [8, 9]. Moreover, LAB are known to produce carotenoids as a strategy for eliminating reactive oxygen species [10, 11]. The yellow pigment produced by

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confidence: 52%

“…Previous studies have reported that carotenoid production in microorganisms improves stress tolerance [14,15]. LAB also exhibit tolerance against multiple stresses, such as envelope stress (lysozyme), high temperature (heat), solvent, bile, acid, salt, and osmotic stresses [16][17][18][19][20].…”

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confidence: 99%

Isolation of Lactobacillus plantarum subsp. plantarum Producing C30 Carotenoid 4,4��-Diaponeurosporene and the Assessment of Its Antioxidant Activity

Kim

Seo

Cha

et al. 2019

Journal of Microbiology and Biotechnology

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“…The effect of environmental factors such as temperature, pH and salinity of the culture medium in the production of total carotenoids and biomass by various microorganisms was evaluated by other authors [30][31][32][33][34][35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Optimization of Total Carotenoid Production by Halorubrum Sp. TBZ126 Using Response Surface Methodology

Hamidi¹

2014

Microb Biochem Technol

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Carotenoids are one of the most diverse and broadly distributed classes of pigments in nature with a high number of biotechnological applications. Carotenoids have a broad range of functions, especially in relation to human health and their role as biological antioxidants. The increasing demand for consumption of natural carotenoids has raised interest in their bio-production. The objective of the present study was the analysis of environmental factors (temperature, pH and salinity) through response surface methodology (RSM) on the total carotenoid production of Halorubrum sp. TBZ126. In addition the effect of light was evaluated. Five levels of temperature, pH, and salinity were selected based on central composite design (CCD) and RSM to reach the optimum values for the cell growth and carotenoid production. Bio-production was carried out in an orbital shaker using a 10% (v/v) inoculum, and agitation at 120 rpm for 9 days in a non-illuminated environment. Dry cell weight was determined and total carotenoid was estimated by spectrophotometer. The production of biomass ranged from 0.04 to 0.84 g/l and the total carotenoid from 0.15 to 10.78 mg/l. The optimum conditions for cell growth and total carotenoid production in Halorubrum sp. TBZ126 cultures, were temperature 31ºC and 32ºC, pH 7.51 and 7.94 and NaCl (w/v) 18.33% and 20.55%, respectively. In conclusion, employing RSM design and under the light exposure as an inducing factor, carotenoid production by Halorubrum sp. TBZ126 was elevated by 45%. Additionally, TBZ126 could produce carotenoids at lower concentrations of NaCl (as low as 2.5%), in the absence of sodium acetate without elevating magnesium sulfate concentration.

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“…Carotenoids are regarded as efficient quenchers of reactive oxygen species, namely singlet oxygen [21][22][23] which are typical cytotoxic by-products of respiratory metabolism. 24 Nevertheless, there is evidence that carotenoid synthesis is additionally involved in light response in nonphototrophic microorganisms. 25 Moreover, transcriptome analysis gave proof for the upregulation of a deoxyribodipyrimidine photo-lyase.…”

Section: Light-induced Metabolic Adaptationmentioning

confidence: 99%

Differential transcriptomic analysis reveals hidden light response in Streptomyces lividans

Koepff

Morschett²,

Busche

et al. 2017

Biotechnology Progress

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Recently, a comprehensive screening workflow for the filamentous bacterium Streptomyces lividans, a highly performant source for pharmaceutically active agents was introduced. This framework used parallelized cultivation in microtiter plates to efficiently accelerate early upstream process development. Focusing on growth performance, cultivation was successfully scaled-up to 1 L stirred tank reactors. However, metabolic adaptation was observed on the transcriptomic level as among others, several genes incorporated in light response were upregulated during bioreactor cultivation. Despite it was assumed that this was attributed to the fact that reactor cultivations were performed in glass vessels exposed to daylight and artificial room light, this setup did not allow distinguishing exclusively between light and other effects. Upon that, the present study directly investigates the influence of light by defined illumination of microtiter plate cultures. Almost identical growth performance was observed for cultures grown in the dark or with illumination. Transcriptomics revealed the upregulation of seven genes of which 6 have previously been described to be relevant for carotenoid synthesis and its regulation. These pigments are effective quenchers of reactive oxygen species. The seventh transcript coded for a photo-lyase incorporated in UV-damage repair of DNA further confirming induced light response. However, this was fully compensated by metabolic adaptation on the transcriptomic level and overall process performance was maintained. Consequently, environmental conditions need extremely careful control and evaluation during in-depth omics analysis of bioprocesses. Otherwise metabolic adaptation induced by such issues can easily be misinterpreted, especially during studies addressing cultivation system comparisons. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:287-292, 2018.

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Aerobic condition increases carotenoid production associated with oxidative stress tolerance inEnterococcus gilvus

Cited by 16 publications

References 25 publications

Isolation of Lactobacillus plantarum subsp. plantarum Producing C30 Carotenoid 4,4��-Diaponeurosporene and the Assessment of Its Antioxidant Activity

Isolation of Lactobacillus plantarum subsp. plantarum Producing C30 Carotenoid 4,4��-Diaponeurosporene and the Assessment of Its Antioxidant Activity

Optimization of Total Carotenoid Production by Halorubrum Sp. TBZ126 Using Response Surface Methodology

Differential transcriptomic analysis reveals hidden light response in Streptomyces lividans

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