Aerobic biodegradation of MtBE in an upflow fixed bed reactor

Bianchi, Emma; Censabella, Irene; Fascetti, Eugenio

doi:10.1002/jctb.2133

Cited by 3 publications

(4 citation statements)

References 24 publications

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“…All silicone tubing used was Masterflex type (Cole Parmer 96400-35). The liquid from the top of the column was recycled through the inlet positioned at the bottom of the column (Bianchi et al 2008). The influent of the system was placed at the base of the column to ensure the homogenization of the mineral salt medium supplemented with 10 mg/l of TCV and 25 mg/l of MP.…”

Section: Degradation Experiments In Tpufrmentioning

confidence: 99%

Removal of methyl parathion and tetrachlorvinphos by a bacterial consortium immobilized on tezontle-packed up-flow reactor

2011

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A tezontle-packed up-flow reactor (TPUFR) with an immobilized bacterial consortium for biological treatment of methyl-parathion and tetrachlorvinphos was evaluated. These organophosphate pesticides are widely used in Mexico for insect and mite control, respectively. With the aim of developing a tool for pesticide biodegradation, four flow rates (0.936, 1.41, 2.19, and 3.51 l/h) and four hydraulic residence times (0.313, 0.206, 0.133, and 0.083 h) were evaluated in a TPUFR. In the bioreactor, with an operating time of 8 h and a flow of 0.936 l/h, we obtained 75% efficiency in the removal of methyl-parathion and tetrachlorvinphos. Their adsorptions in the volcanic rock were 9% and 6%, respectively. It was demonstrated that the removal of pesticides was due to the biological activity of the immobilized bacterial consortium. We confirmed the decrease in toxicity in the treated effluent from the bioreactor through the application of acute toxicity tests on Eisenia foetida. Immobilization of a bacterial consortium using tezontle as a support is innovative and an economical tool for the treatment of mixtures of organophosphorus pesticide residues.

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Section: Degradation Experiments In Tpufrmentioning

confidence: 99%

Removal of methyl parathion and tetrachlorvinphos by a bacterial consortium immobilized on tezontle-packed up-flow reactor

2011

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“…Similarly, Bianchi et al [54] reported 99.98% removal of 27.8 mg MTBE L -1 by a microbial consortium formed by Alcaligenes sp., Pseudomonas sp. and Microbacterium sp.…”

Section: Resultsmentioning

confidence: 89%

“…Among the few known processes for MTBE biodegradation in up-flow, packed-bed reactors, Bianchi et al [54] reported an estimated volumetric MTBE removal rate of 5.56 mg L -1 h -1 at a volumetric MTBE loading rate of 5.6 mg L -1 h -1 . In addition, Acuna-Askar et al [22] reported estimated volumetric MTBE removal rates of 5.35, 8.25 and 15.68 mg L -1 h -1 at volumetric MTBE loading rates of 6.09, 9.83 and 25 mg L -1 h -1 , respectively.…”

Section: Resultsmentioning

confidence: 99%

Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

et al. 2016

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This study investigated the aerobic biodegradation of methyl tertiary-butyl ether (MTBE) by a microbial consortium in a continuous up-flow packed-bed biofilm reactor using tezontle stone particles as a supporting material for the biofilm. Although MTBE is toxic for microbial communities, the microbial consortium used here was able to resist MTBE loading rates up to 128.3 mg L-1 h-1, with removal efficiencies of MTBE and chemical oxygen demand (COD) higher than 90%. A linear relationship was observed between the MTBE loading rate and the MTBE removal rate, as well as between the COD loading rate and the COD removal rate, within the interval of MTBE loading rates from 11.98 to 183.71 mg L-1 h-1. The metabolic intermediate tertiary butyl alcohol (TBA) was not detected in the effluent during all reactor runs, and the intermediate 2-hydroxy butyric acid (2-HIBA) was only detected at MTBE loading rates higher than 128.3 mg L-1 h-1. The results of toxicity bioassays with organisms from two different trophic levels revealed that the toxicity of the influent was significantly reduced after treatment in the packed-bed reactor. The packed-bed reactor system used in this study was highly effective for the continuous biodegradation of MTBE and is therefore a promising alternative for detoxifying MTBE-laden wastewater and groundwater.

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“…BTEX compounds have been shown to interfere with MTBE degradation in pure cultures of bacteria (Deeb et al 2001), although these interactions may not develop in bioreactors populated with mixed bacterial consortia and with the potential to spatially segregate different metabolic processes (Pruden et al 2003; Bianchi et al 2009). Historically, BTEX and chlorinated compounds were present in the groundwater at the North Hollywood site (GeoTracker 2012), but by the start of the bioreactor trial, BTEX compounds were detected only sporadically and at low concentrations, and thus did not appear to interfere with the removal of MTBE.…”

Section: Discussionmentioning

confidence: 99%

Successful treatment of an MTBE-impacted aquifer using a bioreactor self-colonized by native aquifer bacteria

Hicks

Schmidt

Nickelsen³

et al. 2013

Biodegradation

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A field-scale fixed bed bioreactor was used to successfully treat an MTBE-contaminated aquifer in North Hollywood, CA without requiring inoculation with introduced bacteria. Native bacteria from the MTBE-impacted aquifer rapidly colonized the bioreactor, entering the bioreactor in the contaminated groundwater pumped from the site, and biodegraded MTBE with greater than 99 % removal efficiency. DNA sequencing of the 16S rRNA gene identified MTBE-degrading bacteria Methylibium petroleiphilum in the bioreactor. Quantitative PCR showed M. petroleiphilum enriched by three orders of magnitude in the bioreactor above densities pre-existing in the groundwater. Because treatment was carried out by indigenous rather than introduced organisms, regulatory approval was obtained for implementation of a full-scale bioreactor to continue treatment of the aquifer. In addition, after confirmation of MTBE removal in the bioreactor to below maximum contaminant limit levels (MCL; MTBE = 5 μg L−1), treated water was approved for reinjection back into the aquifer rather than requiring discharge to a water treatment system. This is the first treatment system in California to be approved for reinjection of biologically treated effluent into a drinking water aquifer. This study demonstrated the potential for using native microbial communities already present in the aquifer as an inoculum for ex-situ bioreactors, circumventing the need to establish non-native, non-acclimated and potentially costly inoculants. Understanding and harnessing the metabolic potential of native organisms circumvents some of the issues associated with introducing non-native organisms into drinking water aquifers, and can provide a low-cost and efficient remediation technology that can streamline future bioremediation approval processes.

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Aerobic biodegradation of MtBE in an upflow fixed bed reactor

Cited by 3 publications

References 24 publications

Removal of methyl parathion and tetrachlorvinphos by a bacterial consortium immobilized on tezontle-packed up-flow reactor

Removal of methyl parathion and tetrachlorvinphos by a bacterial consortium immobilized on tezontle-packed up-flow reactor

Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

Successful treatment of an MTBE-impacted aquifer using a bioreactor self-colonized by native aquifer bacteria

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