Removal of methyl parathion and tetrachlorvinphos by a bacterial consortium immobilized on tezontle-packed up-flow reactor

Yáñez-Ocampo, Gustavo; Sánchez-Salinas, Enrique; Ortiz-Hernández, Ma. Laura

doi:10.1007/s10532-011-9475-z

Cited by 30 publications

(13 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results showed a decrease in the bacterial population present in the microbial consortium, from about 10 7 CFU g -1 to 10 5 CFU g -1 , as the HRT decreased from 62.6 to 4.08 h. This phenomenon may have been due to the shear stress caused by the fluid flow that tends to wash away the attached biofilm from the tezontle stones and increase as the fluid flow rate increases and the hydraulic retention time decreases. A similar trend was observed by Yañez-Ocampo et al [32] in their studies on the removal of parathion and tetrachlorvinphos by a bacterial consortium immobilized on tezontle stones in a packed-bed reactor. To the best of our knowledge, no biomass results have been reported in the few studies available on MTBE biodegradation in packed-bed reactors operating in continuous mode at different hydraulic retention times.…”

Section: Resultssupporting

confidence: 87%

“…Furthermore, tezontle does not contain toxic chemicals, is physically stable, can be sterilized and reused and is a low-cost material abundant in Mexico, and the presence of micropores in its structure allows for the establishment of microbial microcolonies [27–29]. Its usefulness as a packing material was demonstrated in different bioremediation processes [27,29–32]. …”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

et al. 2016

View full text Add to dashboard Cite

This study investigated the aerobic biodegradation of methyl tertiary-butyl ether (MTBE) by a microbial consortium in a continuous up-flow packed-bed biofilm reactor using tezontle stone particles as a supporting material for the biofilm. Although MTBE is toxic for microbial communities, the microbial consortium used here was able to resist MTBE loading rates up to 128.3 mg L-1 h-1, with removal efficiencies of MTBE and chemical oxygen demand (COD) higher than 90%. A linear relationship was observed between the MTBE loading rate and the MTBE removal rate, as well as between the COD loading rate and the COD removal rate, within the interval of MTBE loading rates from 11.98 to 183.71 mg L-1 h-1. The metabolic intermediate tertiary butyl alcohol (TBA) was not detected in the effluent during all reactor runs, and the intermediate 2-hydroxy butyric acid (2-HIBA) was only detected at MTBE loading rates higher than 128.3 mg L-1 h-1. The results of toxicity bioassays with organisms from two different trophic levels revealed that the toxicity of the influent was significantly reduced after treatment in the packed-bed reactor. The packed-bed reactor system used in this study was highly effective for the continuous biodegradation of MTBE and is therefore a promising alternative for detoxifying MTBE-laden wastewater and groundwater.

show abstract

Section: Resultssupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Similar studies were also carried out by Yañez-Ocampo et al (Yáñez-Ocampo et al 2011 ) using a tezontle-packed up-flow reactor (TPUFR) with an immobilized bacterial consortium for biological treatment of methyl-parathion and tetrachlorvinphos. In the bioreactor, four flow rates (0.936, 1.41, 2.19, and 3.51 l/h) and four hydraulic residence times (0.313, 0.206, 0.133, and 0.083 h) were evaluated.…”

Section: Discussionsupporting

confidence: 61%

“…There are reports on the biodegradation of pesticides using immobilized cells (Ha et al 2009 ; Yáñez-Ocampo et al 2011 ; Abdel-Razek et al 2013 ) and immobilized enzymes (Richins et al 2000 ; Mansee et al 2005 ). However, studies on the degradation of Endosulfan using immobilized cells are limited (Jo et al 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Use of Ca-alginate immobilized Pseudomonas aeruginosa for repeated batch and continuous degradation of Endosulfan

Pradeep

Subbaiah

2016

3 Biotech

View full text Add to dashboard Cite

The current investigation is taken up with the aim of studying repeated batch and continuous degradation of Endosulfan, using Ca-alginate immobilized cells of Pseudomonas aeruginosa isolated from an agricultural soil. The work involves the study of genes and enzymes involved in the degradation of the pesticide and was carried out with an objective of reducing the toxicity of Endosulfan by degrading it to less toxic metabolites. The long-term stability of Endosulfan degradation was studied during its repeated batch degradation, carried out over a period of 35 days. Immobilized cells of Ps. aeruginosa were able to show 60 % degradation of Endosulfan at the end of the 35th cycle with a cell leakage of 642 × 104 Cfu/mL. During continuous treatment, with 2 % concentration of Endosulfan, 100 % degradation was recorded up to 100 mL/h flow rate and with 10 % concentration of the Endosulfan, and 100 and 85 % degradation was recorded at 20 mL/h flow rate and 100 mL/h flow rate, respectively. After degradation of Endosulfan, products were extracted from a large amount of spent medium using two volumes of ethyl acetate and subjected to the LC–MS analysis. Endosulfan lactone and Endosulfan ether were the products of degradation detected by the LCMS analysis. Plasmid curing experiments indicated that genes responsible for the degradation of Endosulfan are present on the chromosome and not on the plasmid, as growth of Ps. aeruginosa was observed on modified non-sulfur medium with Endosulfan after the plasmid was cured with ethidium bromide. The results of PCR indicated that there is no amplified product of ~1350 bp expected for esd gene, in Ps. aeruginosa, although there were some non-specific bands. Enzymatic degradation studies indicated that the enzymes involved in the degradation of Endosulfan are intracellular. With this investigation, it was indicated that immobilized cells of Ps. aeruginosa have the potential to be used in the bioremediation of water contaminated with Endosulfan.

show abstract

“…Cell immobilization was previously used to make pesticide biodegradation processes more efficient (Kadakol, Kamanavalli, & Shouche, 2011;Yañez-Ocampo, Sanchez-Salinas, Jimenez-Tobon, Penninckx, & Ortiz-Hernández, 2009;Yáñez-Ocampo, Sánchez-Salinas, & Ortiz-Hernández, 2011). This provides multiple advantages over conventional biological systems that use free cells.…”

mentioning

confidence: 99%

Enhancing methyl parathion degradation by the immobilization of Burkholderia sp. isolated from agricultural soils

et al. 2017

Self Cite

View full text Add to dashboard Cite

Organophosphate pesticides are of great interest for research because they are currently the most commonly used pesticides. In this study, a bacterial strain capable of completely degrading methyl parathion (MP) was isolated from agricultural soils in central Mexico. This strain was designated strain S5‐2 and was identified as Burkholderia cenocepacia. To increase degradation yields, cells were immobilized on three different supports: powdered zeolite and Opuntia sp. and Agave sp. fibers. The results indicated a significant increase in MP hydrolysis and p‐nitrophenol (PNP) degradation with immobilized cells compared to free cell cultures. Furthermore, immobilized cells were capable of withstanding and degrading higher concentrations of PNP compared to cell suspension cultures. The cell viability in the free cell cultures, as well as PNP degradation, was affected at concentrations greater than 25 mg/L. In contrast, cells immobilized on Opuntia sp. and Agave sp. fibers completely degraded PNP at concentrations of 100 mg/L. To verify that MP solution toxicity was decreased by B. cenocepacia strain S5‐2 via pesticide degradation, we measured the acetylcholinesterase activity, both before and after treatment with bacteria. The results demonstrate that the activity of acetylcholinesterase was unaffected after MP degradation by bacteria.

show abstract

Removal of methyl parathion and tetrachlorvinphos by a bacterial consortium immobilized on tezontle-packed up-flow reactor

Cited by 30 publications

References 41 publications

Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

Use of Ca-alginate immobilized Pseudomonas aeruginosa for repeated batch and continuous degradation of Endosulfan

Enhancing methyl parathion degradation by the immobilization of Burkholderia sp. isolated from agricultural soils

Contact Info

Product

Resources

About