Aerobic and anaerobic glucose metabolism of Phytomonas sp. isolated from Euphorbia characias

Chaumont, François; Schanck, AndréN.; Blum, J. J.; Opperdoes, Frederik

doi:10.1016/0166-6851(94)00141-3

Cited by 45 publications

(27 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Glucose and citrate degradation pathways proposed for the L. plantarum wild-type strain (NCIMB8826) and LDH-defective strain (TF103). [1], LDH; [2], pyruvate formate-lyase; [3], acetaldehyde dehydrogenase; [4], alcohol dehydrogenase; [5], phosphotransacetylase; [6], acetate kinase; [7], pyruvate oxidase; [8], pyruvate decarboxylase; [9], ␣-acetolactate synthase; [10], ␣-acetolactate decarboxylase; [11], 2,3-butanediol dehydrogenase; [12], nonenzymatic decarboxylation; [13], pyruvate carboxylase; [14], malate dehydrogenase; [15], fumarase; [16], fumarate reductase; [17], malolactic enzyme; [18], citrate lyase; [19], oxalacetate decarboxylase; [20], mannitol-1-phosphate dehydrogenase; [21] (28), and the authors hypothesized the involvement of hydroxyisocaproate dehydrogenase activities. However, we did not detect any hydroxyisocaproate dehydrogenase activity in TF103 cell extracts using several ␣-keto acids as substrates.…”

mentioning

confidence: 99%

See 1 more Smart Citation

13C nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL-ldhD double-knockout strain of Lactobacillus plantarum

1996

View full text Add to dashboard Cite

We have examined the metabolic consequences of knocking out the two ldh genes in Lactobacillus plantarum using 13 C nuclear magnetic resonance. Unlike its wild-type isogenic progenitor, which produced lactate as the major metabolite under all conditions tested, ldh null strain TF103 mainly produced acetoin. A variety of secondary end products were also found, including organic acids (acetate, succinate, pyruvate, and lactate), ethanol, 2,3-butanediol, and mannitol.

show abstract

mentioning

confidence: 99%

“…End products were identified by 13 C nuclear magnetic resonance ( Fig. 2; Table 1) according to the method of Chaumont et al (6).…”

mentioning

confidence: 99%

13C nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL-ldhD double-knockout strain of Lactobacillus plantarum

1996

View full text Add to dashboard Cite

show abstract

“…Since, to our knowledge, no reports describe ethanol production in Leishmania, the function of the PDC/IPDC gene in the genus Leishmania deserves further investigation. On the other hand, ethanol production by Crithidia has been previously described (Cazzulo et al 1985;Chaumont et al 1994), denoting that the PDC/IPDC gene product could participate in the alcohol fermentation pathway. We have not investigated the presence of IAA in Crithidia-conditioned media.…”

Section: Discussionmentioning

confidence: 99%

Auxin production by the plant trypanosomatidPhytomonas serpensand auxin homoeostasis in infected tomato fruits

et al. 2014

View full text Add to dashboard Cite

S U M M A R YPreviously we have characterized the complete gene encoding a pyruvate decarboxylase (PDC)/indolepyruvate decarboxylase (IPDC) of Phytomonas serpens, a trypanosomatid highly abundant in tomato fruits. Phylogenetic analyses indicated that the clade that contains the trypanosomatid protein behaves as a sister group of IPDCs of γ-proteobacteria. Since IPDCs are key enzymes in the biosynthesis of the plant hormone indole-3-acetic acid (IAA), the ability for IAA production by P. serpens was investigated. Similar to many microorganisms, the production of IAA and related indolic compounds, quantified by high performance liquid chromatography, increased in P. serpens media in response to amounts of tryptophan. The auxin functionality was confirmed in the hypocotyl elongation assay. In tomato fruits inoculated with P. serpens the concentration of free IAA had no significant variation, whereas increased levels of IAA-amide and IAA-ester conjugates were observed. The data suggest that the auxin produced by the flagellate is converted to IAA conjugates, keeping unaltered the concentration of free IAA. Ethanol also accumulated in P. serpens-conditioned media, as the result of a PDC activity. In the article we discuss the hypothesis of the bifunctionality of P. serpens PDC/IPDC and provide a three-dimensional model of the enzyme.

show abstract

“…There is no nuclear genome sequencing project for Phytomonas, but the absence from its mitochondrial genome of genes encoding universally conserved components of the cytochrome c reductase and cytochrome c oxidase complexes (Maslov et al 1999;Nawathean & Maslov 2000) supports the biochemical evidence that this parasite is incapable of cytochromedependent respiration (Chaumont et al 1994;Maslov et al 1999). Like bloodstream T. brucei, Phytomonas gets by on inefficient (at least in terms of ATP yield) aerobic glucose fermentation because of the availability of a sugar-rich meal ticket.…”

Section: (I) Comparative Metabolism In the Trypanosomatidaementioning

confidence: 99%

Niche metabolism in parasitic protozoa

Ginger

2005

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

Complete or partial genome sequences have recently become available for several medically and evolutionarily important parasitic protozoa. Through the application of bioinformatics complete metabolic repertoires for these parasites can be predicted. For experimentally intractable parasites insight provided by metabolic maps generated in silico has been startling. At its more extreme end, such bioinformatics reckoning facilitated the discovery in some parasites of mitochondria remodelled beyond previous recognition, and the identification of a non-photosynthetic chloroplast relic in malarial parasites. However, for experimentally tractable parasites, mapping of the general metabolic terrain is only a first step in understanding how the parasite modulates its streamlined, yet still often puzzlingly complex, metabolism in order to complete life cycles within host, vector, or environment. This review provides a comparative overview and discussion of metabolic strategies used by several different parasitic protozoa in order to subvert and survive host defences, and illustrates how genomic data contribute to the elucidation of parasite metabolism.

show abstract

Aerobic and anaerobic glucose metabolism of Phytomonas sp. isolated from Euphorbia characias

Cited by 45 publications

References 15 publications

13C nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL-ldhD double-knockout strain of Lactobacillus plantarum

13C nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL-ldhD double-knockout strain of Lactobacillus plantarum

Auxin production by the plant trypanosomatidPhytomonas serpensand auxin homoeostasis in infected tomato fruits

Niche metabolism in parasitic protozoa

Contact Info

Product

Resources

About