Aerobe Glykolyse in mit Poliomyelitis-Viren und ECHO-Virus Typ 9 infizierten Gewebekulturen

Abstract

Experiments on aerobic glycolytic metabolism in tissue cultures during the multiplication of poliomyelitis virus led to varying results. Some authors found the rate unaltered, others observed a transitory, but considerable increase.In this series of experiments aerobic glycolysis was measured after infection of monkey kidney and HeLa cell cultures with all three serotypes of poliomyelitis virus and with ECHO-Virus type 9. Virus multiplication was measured and the cytopathogenic effect observed in the same cultures.At the time of inoculation the cell cultures had varying rates of glycolysis, some none at all. In none of the experiments was virus multiplication associated with a change in glycolytic rate. In some cases a transitory rate increase could be observed after the completion of virus liberation. This phenomenon is not a result of virus multiplication but probably of cytolysis. The capability of glycolytic rate increase in the cell-cultures used was proved with 2.4-dinitrophenol.After the end of the virus multiplication cycle the rate of aerobic glycolysis does not drop suddenly, as does that of respiration and anaerobic glycolysis. This finding is considered to show that respiration and anaerobic glycolysis play the essential part in the energy-donating metabolism of cell-cultures.The increase of the glycolytic rate during enterovirus infection described in the literature is nonspecific with respect to virus multiplication.