Adventitious bud formation in culture of Douglas fir (Pseudotsuga menziesii (MIRB.) Franco)

Cheng, Tsai-Ying

doi:10.1016/0304-4211(75)90049-8

Cited by 113 publications

(27 citation statements)

References 3 publications

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“…To determine if protein differences observed in the cotyledons were medium-specific, embryos were also placed on a half-strength variation of the medium of Linsmaier and Skoog as described by Cheng [2] supplemented with either 5 #M (1/2 L) or 2.5 #M (1/2 L 1/2 H) each of indole acetic acid (IAA), indole butyric acid (IBA), 2,y-y dimethylallylaminopurine (2iP) and BA. In addition, embryos were placed on 1/2 S without hormones (aux:g) or with only IAA and IBA (aux:~) to confirm that the changes observed were a result of a BA-supplemented medium.…”

Section: Methodsmentioning

confidence: 99%

SDS-PAGE analysis of bud-forming cotyledons of Pinus ponderosa

Ellis

Judd

1987

Plant Cell Tiss Organ Cult

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The placement of Pinus ponderosa embryos on a benzyladenine (BA) supplemented medium induces the differentiation of two distinct morphological types of cotyledons. Cotyledons in contact with the medium (lower cotyledons) do not elongate, and rapidly respond to BA by forming multiple buds along their entire length. Cotyledons not in contact with the medium (upper cotyledons) elongate, do not form buds, and are similar both morphologically and in protein profiles to seedling cotyledons that do not respond to BA. Using sodium dodecyl sulfate polyacrylamide electrophoresis, protein differences between these two cotyledon types can be detected as early as one week. These protein differences include the reduced level of a 52,000 Kilodalton (52 Kd) peptide and the appearance of a 4 Kd peptide in the lower cotyledons. These peptide differences may prove useful as markers for tissue that will respond to BA.

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Section: Methodsmentioning

confidence: 99%

SDS-PAGE analysis of bud-forming cotyledons of Pinus ponderosa

Ellis

Judd

1987

Plant Cell Tiss Organ Cult

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“…con un medio libre de hormonas y las plántulas en un sustrato con turba y perlita, tuvieron un 20 % de formación de raíces. En Pseudotsuga menziesii el crecimiento de tallos adventicios fue estimulado por un medio de cultivo de baja concentración de sales inorgánicas sin adición de reguladores de crecimiento (Cheng, 1975). En las plántulas provenientes de los tratamientos con azúcar al 3 % y sacarosa al 3 % que se transfirieron al sustrato, la supervivencia fue nula a los 25 días, sobreviviendo una plántula a los 29 días cumplidos proveniente del tratamiento con sacarosa al 6 %, ésta formó raíz con la ayuda de hongos ectomicorrízicos (AGROTERRA), aunque su crecimiento fue muy lento.…”

Section: Resultsunclassified

“…shoots with a hormone-free medium and the plantlets in a substrate with peat and perlite; they had 20 % root formation. In Pseudotsuga menziesii, growth of adventitious shoots was stimulated by a culture medium with low concentrations of inorganic salts without growth regulators added (Cheng, 1975). In the plantlets from treatments with 3 % sugar and 3 % sucrose that were transferred to substrate, survival was zero at 25 days.…”

Section: Conclusionesmentioning

confidence: 99%

MORFOGÉNESIS IN VITRO DE Pseudotsuga menziesii VAR. glauca

Carrillo-Benítez¹,

O²,

Álvarez-Moctezuma³

2011

Rchscfa

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RESUMENSe evaluó la respuesta morfogénica a partir de embriones cigóticos cultivados in vitro de semilla almacenada (un año) de Pseudotsuga menziesii var. glauca recolectada en Tlaxcala. Las semillas fueron desinfestadas con detergente y H 2 O 2 (3 % v/v) durante 48 h en agitación a 50 rpm, cultivadas en el medio de Murashige y Skoog (1962) sin reguladores. La germinación ocurrió después de siete días y posteriormente subcultivados a un medio MS con 2,4-D (3 mg·L -1 ) y BA (1 mg·L -1 ). Con los callos obtenidos en un medio HS se evaluaron tres concentraciones de ABA para promover formación de estructuras embrionarias, presentándose el mejor tratamiento con concentración de 10.0 mg·L -1 (P<.0001). El mejor desarrollo de plántulas se presentó empleando un medio Murashige y Skoog (1962) con sacarosa al 6 %. Se usaron micorrizas para mejor adaptación de plántulas a suelo. No hubo formación de raíces. ABSTRACT L-1 (P<.0001). The best plantlet development occurred using Murashige and Skoog (1962) medium with 6% sucrose. Mycorrhizae were used to improve plantlet adaptation to soil. No roots formed.PALABRAS CLAVE: Embrión, callo, plántula, reguladores del crecimiento.

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“…A half-strength Cheng medium [9] supplemented with 0.49#M BA, 5.1#M IAA and 4.9/~M IBA and a half-strength SH medium supplemented with 2.2/~M BA were used successfully. Ellis & Bilderback [12] hypothesized the reduction in total nitrogen in the half-strength medium enhanced caulogenesis.…”

Section: Caulogenesismentioning

confidence: 99%

Influence of plant growth regulators, basal media and carbohydrate levels on the in vitro development of Pinus ponderosa (Dougl. ex Law.) cotyledon explants

Tuskan

Sargent

Rensema

et al. 1990

Plant Cell Tiss Organ Cult

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Applications of in vitro screening techniques for Pinus ponderosa resistance to Peridermium harknessii could be beneficial in a tree breeding program. Plant growth regulators, basal media formula and carbohydrate levels were examined to determine the various effects each would have on excised cotyledon growth and development. Proliferating green callus was initiated from cotyledon explants on SH basal medium containing 4.4 #M BA: 5.4 ~tM NAA and 1% sucrose. Subsequent subculturing onto LS medium supplemented with 44.0/tM BA:5.4#M NAA and 2% sucrose improved callus maintenance. The highest frequency of caulogenesis from cotyledon explants occurred on a modified GD medium containing 44.0/~M BA:0.054 #M NAA and 4% glucose. The influence of nitrogen source, osmoticum and medium salt concentrations are discussed relative to callus initiation and caulogenesis.

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Adventitious bud formation in culture of Douglas fir (Pseudotsuga menziesii (MIRB.) Franco)

Cited by 113 publications

References 3 publications

SDS-PAGE analysis of bud-forming cotyledons of Pinus ponderosa

SDS-PAGE analysis of bud-forming cotyledons of Pinus ponderosa

MORFOGÉNESIS IN VITRO DE Pseudotsuga menziesii VAR. glauca

Influence of plant growth regulators, basal media and carbohydrate levels on the in vitro development of Pinus ponderosa (Dougl. ex Law.) cotyledon explants

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