SDS-PAGE analysis of bud-forming cotyledons of Pinus ponderosa

Ellis, David; Judd, Ralph C.

doi:10.1007/bf00036576

Cited by 25 publications

(17 citation statements)

References 16 publications

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“…2). The overall pattern of variations revealed after seven days of culture by RDD analysis is in good agreement with previous results obtained with less resolving, protein-based approaches [7,[12][13][14]19], and indicates that hormoneindependent transcriptional modulation (mainly downregulation) is the most common response. The predominating event during early phases of in vitro culture thus appears to be a reprogramming of gene expression that occurs regardless of the particular developmental fate of the explants.…”

Section: Discussionsupporting

confidence: 78%

“…In fact, the largest fraction of differential bands (58%) was found to correspond to mRNAs that are expressed in to control cotyledons and equally disappear after the establishment of either shoot or callus programmes. This hormone-independent downregulation most likely reflects the de-differentiation process which accompanies the initial stages of in vitro culture and involves, among others, a striking alteration of cotyledon physiology with the concomitant negative modulation of several chloroplast proteins [7,12,19,40]. Within the same mRNA population, a smaller fraction of amplicons corresponding to newly expressed genes (28%) was detected.…”

Section: Resultsmentioning

confidence: 98%

“…A common outcome of these studies, based mainly on protein analyses, has been the detection of several protein species that non-specifically decrease or disappear during the first stages of in vitro culture. The most significant of these changes involve components of the photosynthetic apparatus, with a generalized reduction of ribulose 1,5-bisphosphate carboxylase oxygenase subunits [7,12,19,40]. …”

Section: Introductionmentioning

confidence: 99%

“…Very few protein or mRNA species have been found to be synthesized ex novo under in vitro regeneration conditions, and some of them have been proposed as potential somatic organogenesis [12,22,30,31] or embryogenesis [1,10,19,36,37,39] markers. In a tobacco thin cell layer system, six genes that are induced in a non-tissue-specific manner during in vitro floral initiation have been isolated [26].…”

Section: Introductionmentioning

confidence: 99%

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New potential markers of in vitro tomato morphogenesis identified by mRNA differential display

et al. 1996

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The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such genes and to monitor changes in gene expression accompanying in vitro regeneration, we have adapted the mRNA differential display technique to the comparative analysis of a model system of tomato cotyledons that can be driven selectively toward either shoot or callus formation by means of previously determined growth regulator supplementations. Hormone-independent transcriptional modulation (mainly down-regulation) has been found to be the most common event, indicating that a non-specific reprogramming of gene expression quantitatively predominates during the early phases of in vitro culture. However, cDNA fragments representative of genes that are either down-regulated or induced in a programme-specific manner could also be identified, and two of them (G35, G36) were further characterized. One of these cDNA fragments, G35, corresponds to an mRNA that is down-regulated much earlier in callus- (day 2) than in shoot-determined explants (day 6). The other, G36, identifies an mRNA that is transiently expressed in shoot-determined explants only, well before any macroscopic signs of differentiation become apparent, and thus exhibits typical features of a morphogenetic marker.

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Section: Discussionsupporting

confidence: 78%

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

New potential markers of in vitro tomato morphogenesis identified by mRNA differential display

et al. 1996

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“…These are proteins 6,7,33,34 (Fig. 4) germination phase-abundant category in the in-vitro-germinating embryos, and thus declined in relative rate of synthesis after d 3 to d 5.…”

Section: Changes In Protein Composition and Synthesis In Embryos Germmentioning

confidence: 99%

Changes in Protein Synthesis upon Cytokinin-Mediated Adventitious Bud Induction and during Seedling Development in Norway Spruce, Picea abies

Stabel¹,

Eriksson²,

Engström³

1990

Plant Physiol.

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A pulse-treatment of embryos of Picea abies (L.) Karst with cytokinin efficiently and reproducibly induces the coordinate de novo formation of bud primordia from subepidermal cells. The cytokinin treatment also affects the germinative development of the embryo; chloroplast maturation is delayed, and cell elongation is completely suppressed. We have analyzed the protein patterns in developing spruce embryos with the aim of identifying proteins which are differentially synthesized during early bud-differentiation and germination. In addition to a set of major seed storage proteins and a large set of constitutively synthesized proteins, we distinguish two sets of proteins that showed different patterns of synthesis in relation to germination. One was synthesized at high rates during germination, and the second set during postgerminative seedling development. Twenty-two proteins were differentially synthesized in the bud-induced versus the germinating embryos. Interestingly, all 22 belonged to either the germination phase-abundant or the seedling protein sets, whereas the constitutively synthesized proteins were unaffected by the treatment. Proteins synthesized exclusively in bud-induced embryos were not found. In total, the bud-induction treatment caused a maintenance of a protein synthesis pattem typical for the germination phase in the nontreated embryos, and the de novo formation of buds was not preceded by a major change in gene expression in the tissue.

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Temporal Competence of Embryonic Pinus Ponderosa Cotyledons to Form Multiple Buds in Vitro

Ellis

Bilderback

1989

American J of Botany

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To investigate temporal changes occurring during tissue maturation and loss of organogenic competence, embryos of Pinus ponderosa Laws, were excised from seed and placed horizontally on medium in sterile culture. On a hormone‐free basal medium, cotyledons differentiated and failed to form multiple buds. When placed on a benzyladenine (BA)‐containing medium, the lower cotyledons in contact with the medium formed multiple buds while those elevated above the medium did not. Cotyledons became incompetent to form buds when embryos were initially placed on basal medium for 2 days before being transferred to the BA‐supplemented medium. In order to initiate buds, cotyledons of newly excised embryos had to be exposed to BA for at least 3 days. Exposure to BA for longer than 7 days did not significantly increase the number of cotyledonary buds. Cotyledons elevated above the BA‐supplemented medium retained organogenic competence to form buds for up to 14 days but did not form buds unless the cotyledons were placed in contact with the medium. The presence of BA is required to retard cotyledon maturation and sustain tissue competence to initiate buds.

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SDS-PAGE analysis of bud-forming cotyledons of Pinus ponderosa

Cited by 25 publications

References 16 publications

New potential markers of in vitro tomato morphogenesis identified by mRNA differential display

New potential markers of in vitro tomato morphogenesis identified by mRNA differential display

Changes in Protein Synthesis upon Cytokinin-Mediated Adventitious Bud Induction and during Seedling Development in Norway Spruce, Picea abies

Temporal Competence of Embryonic Pinus Ponderosa Cotyledons to Form Multiple Buds in Vitro

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