Advantages and limitations of coupling isotachophoresis and comprehensive isotachophoresis–capillary electrophoresis to time-of-flight mass spectrometry

Peterson, Zlatuše D.; Bowerbank, Christopher R.; Collins, David C.; Graves, Steven W.; Lee, Milton L.

doi:10.1016/s0021-9673(03)00235-8

Cited by 47 publications

(60 citation statements)

References 37 publications

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“…The influence of flow rates of nebulizing and drying gas has been evaluated separately for coaxial sheath flow interfaces in different setups [95]. Especially elevated flow rates of nebulizing gas are known to cause a hydrodynamic flow inside the CE separation capillary due to an aspiration effect caused by a pressure drop at the capillary tip [26,[95][96][97]. Consequently, resolution of proteins and peptides gets impaired and sometimes is completely abolished (Figs.…”

Section: Influence Of Instrumental Parameters On Esi Processmentioning

confidence: 99%

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

2005

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High throughput, outstanding certainty in peptide/protein identification, exceptional resolution, and quantitative information are essential pillars in proteome research. Capillary electrophoresis (CE) coupled to mass spectrometry (MS) has proven to meet these requirements. Soft ionization techniques, such as matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI), have paved the way for the story of success of CE-MS in the analysis of biomolecules and both approaches are subject of discussion in this article. Meanwhile, CE-MS is far away from representing a homogeneous field. Therefore the review will cover a vast area including the coupling of different modes of CE (capillary zone electrophoresis, capillary isoelectric foscusing, capillary electrochromatography, micellar electrokinetic chromatography, nonaqueous capillary electrophoresis) to MS as well as on-line preconcentration techniques (transient capillary isotachophoresis, solid-phase extraction, membrane preconcentration) applied to compensate for restricted detection sensitivity. Special attention is given to improvements in interfacing, namely addressing nanospray and coaxial sheath liquid design. Peptide mapping, collision-induced dissociation with subsequent tandem MS, and amendments in mass accuracy of instruments improve information validity gained from MS data. With 2-D on-line coupling of liquid chromatography (LC) and CE a further topic will be discussed. A special section is dedicated to recent attempts in establishing CE-ESI-MS in proteomics, in the clinical and diagnostic field, and in the food sector.

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Section: Influence Of Instrumental Parameters On Esi Processmentioning

confidence: 99%

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

2005

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“…Thus, ITP is mainly used as an on-line preconcentration step prior to CZE (transient-ITP). This is clearly shown in a recent publication by Peterson et al [66]. For low concentration of peptides ITP caused discrimination in the ESI-MS, whereas the comprehensive ITP-CZE-MS approach offered a loadability of 10 mL in conjunction with separation in the CZE and on-line MS detection.…”

Section: Itp-msmentioning

confidence: 88%

“…The combination of two highly resolving and orthogonal separation techniques of CIEF and RPLC or CZE, together with analyte focusing and concentration, significantly enhances the dynamic range and sensitivity of conventional MS towards the identification of low-abundance ITP-CZE not only enables the increase of loadability but can be used in a comprehensive way, as shown in an ITP-CZE-MS approach by Peterson et al [66]. However, the ITP as the first dimension does not show the resolution as, e.g., the CIEF-approach.…”

Section: Applications To Multidimensional and Integrated Approaches Cmentioning

confidence: 99%

On‐line capillary electrophoresis‐mass spectrometry for the analysis of biomolecules

et al. 2004

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Mass spectrometry (MS) has become a key tool for the characterization of biologically relevant molecules in the last decade. Due to the complexity of most biological samples an upstream separation is essential. Capillary electrophoresis (CE) has gained much interest due to its high separation efficiency, speed, and often complementary selectivity to liquid chromatography. We describe the state-of-the-art of on-line CE-MS for the analysis of molecules of biological origin. The characterization of peptides, including the study of post-translational modifications, intact proteins, oligonucleotides, and related interaction studies are reviewed. Relevant publications are summarized in tables, including some important method parameters. Key applications are discussed with respect to the advantages and limitations of CE-MS. Coupling interfaces, preconcentration techniques, capillary coatings, and the different CE techniques, e.g., capillary zone electrophoresis, capillary isoelectric focusing, capillary gel electrophoresis, etc. are briefly discussed against the background of their bioanalytical applications.

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“…Comprehensive ITP-CZE was successfully coupled to electrospray ionization orthogonal acceleration time-of-flight mass spectrometry using angiotensin peptides as model analytes (Peterson et al, 2003). ITP-TOF-MS alone was adequate for the separation and detection of high concentration samples.…”

Section: Itp-czementioning

confidence: 99%

Column Coupling Electrophoresis in Biomedical Analysis

Mikuš¹,

Maráková²

2011

Biomedical Engineering - From Theory to Applications

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Advantages and limitations of coupling isotachophoresis and comprehensive isotachophoresis–capillary electrophoresis to time-of-flight mass spectrometry

Cited by 47 publications

References 37 publications

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

On‐line capillary electrophoresis‐mass spectrometry for the analysis of biomolecules

Column Coupling Electrophoresis in Biomedical Analysis

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