Advantage of fat-derived CD73 positive cells from multiple human tissues, prospective isolated mesenchymal stromal cells

Abstract

Somatic stem cells have been isolated from multiple human tissues for their potential usefulness in cell therapy. Currently, mesenchymal stromal cells (MSCs) are prepared after several passages requiring a few months of cell culture. In this study, we used a prospective isolation method of somatic stem cells from gestational or fat tissues, which were identified using CD73 antibody. CD73-positive population from various tissues existed individually in flowcytometric pattern, especially subcutaneous fat- and amniotic-derived cells showed the highest enrichment of CD73-positive cells. Moreover, the cell populations isolated with the prospective method showed higher proliferative capacity and stem cell marker expression, compared to the cell populations which isolated through several passages of culturing whole living cells: which we named “conventional method” in this paper. Furthermore, the therapeutic potential of CD73-positive cells was evaluated in vivo using a mouse model of pulmonary fibrosis. After intranasal administration, murine CD73-positive cells reduced macrophage infiltration and inhibited fibrosis development. These results suggest that further testing using CD73-positive cells may be beneficial to help establish the place in regenerative medicine use.

Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation

“…We then characterized the phenotype of hASCs via fluorescenceactivated cell sorting analysis in accordance with the low expression of surface makers including CD14, CD34, and CD45 and high expression of major surface marker including CD73 and CD105. [53][54][55][56] As predicted, high expression of CD73 and CD105 and low expression of CD14, CD34, and CD45 were observed (Figure S3), consistent with that in prior studies. 53,57,58 To observe cell viability and the adhesion pattern of hASCs grown on a culture plate, PDMS, and IA-PDMS, the Live/Dead assay was used.…”

Polychromatic Flow Cytometric Analysis of Stromal Vascular Fraction from Lipoaspirate and Microfragmented Counterparts Reveals Sex-Related Immunophenotype Differences

“…However, CD73 expression on MSC has been associated with their anti-inflammatory effects and reparative properties [ 28 ], and on unpassaged subcutaneous adipose tissue MSC, the expression of CD73 gave a higher proliferative capacity and increased stem cell marker expression [ 29 ].…”

CD73-positive cell spheroid transplantation attenuates colonic atrophy

“…The copyright holder for this preprint this version posted August 24, 2022. ; https://doi.org/10. 1101/2022 We previously demonstrated that cluster of differentiation (CD)73 molecules can be used to efficiently isolate cell populations with MSC characteristics from human and rodent adipose tissues (Suto et al, 2017;Suto et al, 2020). However, the specific characteristics of CD73 + cells remain unclear.…”