Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation

Abstract

Medical devices made from poly(dimethylsiloxane) (PDMS)‐based silicone implants have been broadly used owing to their inert properties, biocompatibility, and low toxicity. However, long‐term implantation is usually associated with complications, such as capsular contracture due to excessive local inflammatory response, subsequently requiring implant removal. Therefore, modification of the silicone surface to reduce a risk of capsular contracture has attracted increasing attention. Human adipose‐derived stem cells (hASCs) are known to provide potentially therapeutic applications for tissue engineering, regenerative medicine, and reconstructive surgery. Herein, hASCs coating on a PDMS (hASC‐PDMS) or itaconic acid (IA)‐conjugated PDMS (hASC‐IA‐PDMS) surface is examined to determine its biocompatibility for reducing capsular contracture on the PDMS surface. In vitro cell cytotoxicity evaluation showed that hASCs on IA‐PDMS exhibit higher cell viability than hASCs on PDMS. A lower release of proinflammatory cytokines is observed in hASC‐PDMS and hASC‐IA‐PDMS compared to the cells on plate. Multiple factors, including in vivo mRNA expression levels of cytokines related to fibrosis; number of inflammatory cells; number of macrophages and myofibroblasts; capsule thickness; and collagen density following implantation in rats for 60 days, indicate that incorporated coating hASCs on PDMSs most effectively reduces capsular contracture. This study demonstrates the potential of hASCs coating for the modification of PDMS surfaces in enhancing surface biocompatibility for reducing capsular contracture of PDMS‐based medical devices.

Porous Polymer Structures with Tunable Mechanical Properties Using a Water Emulsion Ink

“…The ability to tune porosity, mechanical properties, and rheology implies a significant and broad impact of this work across diverse industries where PDMS is used frequently. This work stands to benefit the state-of-the-art in medical use, combining tunable mechanical properties with improvements in antimicrobial properties, antibacterial properties, and reducing capsular contracture that leads to implant failure [ 42 , 43 , 44 ]. The potential of adding self-replenishing hydrophobic coatings to this tunable material is notable to the space industry, especially considering the current resistance of these materials in a low earth orbit environment [ 45 , 46 ].…”

Effective wound healing on diabetic mice by adhesive antibacterial GNPs-lysine composited hydrogel

“…Isolation and characterization methods of hASCs are describe in our previous publication. 70 hASCs were cultivated utilizing Dulbecco’s modified Eagle’s medium low-glucose (Low-DMEM) (obtained from HyClone Laboratories, Logan, UT), supplemented with 10% fetal bovine serum (FBS) (HyClone) and 1% antibiotic/antimycotic solution (HyClone). The harvested cultured cells were detached using a 0.25% trypsin solution (HyClone) at a concentration of 1×.…”

Effect of ASC Injection in the Inflammatory Reaction in Silicone Implant Capsule: Animal Model

“…On the other hand, in clinical research, a few studies analyzed the effect of fat transfer to prevent capsular contraction, 13 , 14 with promising results. Sutthiwanjampa et al 15 have analyzed the effect of mesenchymal stem cells as a therapy to prevent capsular contracture in an in vitro scenario. In light of these factors, our group hypothesized the use of adipocyte stem cells (ASC) as an autologous therapy to prevent capsular contracture in an in vivo model.…”