Advancing Towards a Tissue-engineered Tympanic Membrane: Silk Fibroin as a Substratum for Growing Human Eardrum Keratinocytes

Ghassemifar, Reza; Redmond, Sharon L.; Zainuddin, Zainuddin -; Chirilă, Traian V.

doi:10.1177/0885328209104289

Cited by 55 publications

(36 citation statements)

References 34 publications

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“…Therapeutic structures in the form of planar sheets or membranes represent a particularly attractive architecture for tissue repair, as these constructs are well suited towards applications such as wound healing and large area tissue regeneration. The formation of therapeutic films by solution casting or electrospinning of naturally derived silk fibroin and by multilayer complexation of charged polypeptides has been studied for over two decades [82-85]. These membranes have demonstrated the potential ability to act as scaffolds for cell growth as well as for drug release and protein immobilization but will not be discussed here, as they do not incorporate synthetic peptides.…”

Section: Peptide-based Membranes For Biological Applicationsmentioning

confidence: 99%

Peptide self-assembly for crafting functional biological materials

Matson

Zha²,

Stupp

2011

Current Opinion in Solid State and Materials Science

257

190

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Self-assembling, peptide-based scaffolds are frontrunners in the search for biomaterials with widespread impact in regenerative medicine. The inherent biocompatibility and cell signaling capabilities of peptides, in combination with control of secondary structure, has led to the development of a broad range of functional materials with potential for many novel therapies. More recently, membranes formed through complexation of peptide nanostructures with natural biopolymers have led to the development of hierarchically-structured constructs with potentially far-reaching applications in biology and medicine. In this review, we highlight recent advances in peptide-based gels and membranes, including work from our group and others. Specifically, we discuss the application of peptide-based materials in the regeneration of bone and enamel, cartilage, and the central nervous system, as well as the transplantation of islets, wound-healing, cardiovascular therapies, and treatment of erectile dysfunction after prostatectomy

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Section: Peptide-based Membranes For Biological Applicationsmentioning

confidence: 99%

Peptide self-assembly for crafting functional biological materials

Matson

Zha²,

Stupp

2011

Current Opinion in Solid State and Materials Science

257

190

View full text Add to dashboard Cite

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“…Following seminal studies by Minoura’s group (Minoura et al 1990,1995a, b), the silk proteins, especially the fibroin produced by the B. mori silkworm, have been widely investigated as potential biomaterials and considered for tissue engineering applications (Altman et al 2003; Wang et al 2006; Vepari and Kaplan 2007; Hakimi et al 2007; Kearns et al 2008; Kundu et al 2008; Wang et al 2009; Murphy and Kaplan 2009; Hardy and Scheibel 2010; Numata and Kaplan 2010; Ghassemifar et al 2010; Harkin et al 2011; Pritchard and Kaplan 2011; Wenk et al 2011; Gil et al 2013). …”

Section: Introductionmentioning

confidence: 99%

Evaluation of silk sericin as a biomaterial: in vitro growth of human corneal limbal epithelial cells on Bombyx mori sericin membranes

et al. 2013

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Sericin and fibroin are the two major proteins in the silk fibre produced by the domesticated silkworm, Bombyx mori. Fibroin has been extensively investigated as a biomaterial. We have previously shown that fibroin can function successfully as a substratum for growing cells of the eye. Sericin has been so far neglected as a biomaterial because of suspected allergenic activity. However, this misconception has now been dispelled, and sericin’s biocompatibility is currently indisputable. Aiming at promoting sericin as a possible substratum for the growth of corneal cells in order to make tissue-engineered constructs for the restoration of the ocular surface, in this study we investigated the attachment and growth in vitro of human corneal limbal epithelial cells (HLECs) on sericin-based membranes. Sericin was isolated and regenerated from the silkworm cocoons by an aqueous procedure, manufactured into membranes, and characterized (mechanical properties, structural analysis, contact angles). Primary cell cultures from two donors were established in serum-supplemented media in the presence of murine feeder cells. Membranes made of sericin and fibroin-sericin blends were assessed in vitro as substrata for HLECs in a serum-free medium, in a cell attachment assay and in a 3-day cell growth experiment. While the mechanical characteristics of sericin were found to be inferior to those of fibroin, its ability to enhance the attachment of HLECs was significantly superior to fibroin, as revealed by the PicoGreen® assay. Evidence was also obtained that cells can grow and differentiate on these substrata.Electronic supplementary materialThe online version of this article (doi:10.1186/2194-0517-2-14) contains supplementary material, which is available to authorized users.

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“…However, it is difficult to treat a chronic TM perforation due to decreased regenerative activity of the TM at the margin of the perforation. In an effort to overcome this drawback, the use of the novel adjuvant scaffolds such as collagen [5], calcium alginate [6], silk [7], and chitosan [8][9][10][11] have been tried as patch. However, only bioscaffold showed a limited rate of healing in a chronic TM perforation study, similar to the rate of the paper patch technique.…”

Section: Introductionmentioning

confidence: 99%