Advancing the Zebrafish embryo test for endocrine disruptor screening using micro‐injection: Ethinyl estradiol as a case study

Michiels, Ellen D. G.; Vergauwen, Lucia; Lai, Foon Yin; Town, Raewyn M.; Nuijs, Alexander L.N. van; Cruchten, Steven Van; Knapen, Dries

doi:10.1002/etc.4343

Cited by 7 publications

(5 citation statements)

References 44 publications

(69 reference statements)

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“…), and the diffusion coefficient of the target compound is invariant with time. In addition to the environmental aspects detailed herein, the conceptual framework is applicable in a range of contexts including, e.g., design of dosing systems for toxicity testing of poorly soluble compounds. , Our findings highlight the crucial role of the size of the plastic particle in determining the uptake/release kinetics of associated molecules. This fundamental aspect is currently ignored in the context of developing standard test procedures for assessing migration of contaminants in plastics, e.g., with regard to human exposure via food packaging or toys. − …”

Section: Resultsmentioning

confidence: 92%

Uptake and Release Kinetics of Organic Contaminants Associated with Micro- and Nanoplastic Particles

Town

Leeuwen

2020

Environ. Sci. Technol.

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A generic theoretical framework is presented for describing the kinetics of uptake and release of organic compounds that associate with plastic particles. The underlying concepts account for the physicochemical features of the target organic compounds and the plastic particles. The developed framework builds on concepts established for dynamic speciation analysis by solid-phase microextraction and the size-dependent reactivity features of particulate complexants. The theoretical framework is applied to interpretation of literature data, thereby providing more rigorous insights into previous observations. The presented concepts enable predictions of the sink/source functioning of plastic particles and their impact on the dynamic chemical speciation of organic compounds in aqueous environmental media and within biota. Our results highlight the fundamental influence of particle size on the uptake and release kinetics. The findings call for a comprehensive description of the physicochemical features of plastic particles to be provided in experimental studies on micro- and nanoplastics in different types of aquatic environmental media.

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Section: Resultsmentioning

confidence: 92%

Uptake and Release Kinetics of Organic Contaminants Associated with Micro- and Nanoplastic Particles

Town

Leeuwen

2020

Environ. Sci. Technol.

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“…However, most laboratories use aquatic exposure in screening campaigns with ZF larvae, thus neglecting the potentially hindered uptake and absorption of lipophilic compounds which, in turn, can lead to a rather high rate of false negatives. More recently, some groups proposed to administer lipophilic compounds via microinjection into yolk sac due to easy and straightforward microinjection protocols that can be automated if required [ 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Drug Administration Routes Impact the Metabolism of a Synthetic Cannabinoid in the Zebrafish Larvae Model

et al. 2020

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Zebrafish (Danio rerio) larvae have gained attention as a valid model to study in vivo drug metabolism and to predict human metabolism. The microinjection of compounds, oligonucleotides, or pathogens into zebrafish embryos at an early developmental stage is a well-established technique. Here, we investigated the metabolism of zebrafish larvae after microinjection of methyl 2-(1-(5-fluoropentyl)-1H-pyrrolo[2,3-b]pyridine-3-carboxamido)-3,3-dimethylbutanoate (7′N-5F-ADB) as a representative of recently introduced synthetic cannabinoids. Results were compared to human urine data and data from the in vitro HepaRG model and the metabolic pathway of 7′N-5F-ADB were reconstructed. Out of 27 metabolites detected in human urine samples, 19 and 15 metabolites were present in zebrafish larvae and HepaRG cells, respectively. The route of administration to zebrafish larvae had a major impact and we found a high number of metabolites when 7′N-5F-ADB was microinjected into the caudal vein, heart ventricle, or hindbrain. We further studied the spatial distribution of the parent compound and its metabolites by mass spectrometry imaging (MSI) of treated zebrafish larvae to demonstrate the discrepancy in metabolite profiles among larvae exposed through different administration routes. In conclusion, zebrafish larvae represent a superb model for studying drug metabolism, and when combined with MSI, the optimal administration route can be determined based on in vivo drug distribution.

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“…Six-month-old zebrafish (Danio rerio, AB strain) were reared in an automatic housing system (ZebTec standalone, Tecniplast, Buguggiate, Italy) with a 14:10 h light: dark cycle and recirculating, biologically filtered water (28 ± 0.2 • C, pH 7.5 ± 0.3 and conductivity 500 ± 15 µS/cm), as previously described [24]. Reconstituted freshwater (45 mg/L CaCO 3 ) was prepared by adding Instant Ocean Sea Salt (Instant Ocean) to reverse-osmosis water (RO 40; Werner).…”

Section: Sample Collectionmentioning

confidence: 99%

Lipidomics profiling of zebrafish liver through untargeted liquid chromatography‐high resolution mass spectrometry

Silva

Iturrospe

Boom

et al. 2022

J of Separation Science

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Lipidomics analysis of zebrafish tissues has shown promising results to understand disease‐related outcomes of exposure to toxic substances at a molecular level. However, knowledge about their lipidome is limited, as most untargeted studies only identify the lipids that are statistically significant in their setup. In this work, liquid chromatography‐high resolution mass spectrometry was used to study different aspects of the analytical workflow, that is, extraction solvents (methanol/chloroform/water (3/2/2, v/v/v), methanol/dichloromethane/water (2/3/2, v/v/v) and methanol/methyl‐tert‐butyl ether/water (3/10/2.5, v/v/v), instrumental response, and strategies used for lipid annotation. The number of high‐quality features (relative standard deviation of the intensity values ≤ 10% in the range 103−107 counts) was affected by the dilution of lipid extracts, indicating that it is an important parameter for developing untargeted methods. The workflows used allowed the selection of a dilution factor to annotate 712 lipid species (507 bulk lipids) in zebrafish liver using four software (LipidMatch, LipidHunter, MS‐DIAL, and Lipostar). Retention time mapping was a valuable tool to filter lipid annotations obtained from automatic software annotations. The lipid profiling of zebrafish livers will help in a better understanding of the true constitution of their lipidome at the species level, as well as in the use of zebrafish in toxicological studies.

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Advancing the Zebrafish embryo test for endocrine disruptor screening using micro‐injection: Ethinyl estradiol as a case study

Cited by 7 publications

References 44 publications

Uptake and Release Kinetics of Organic Contaminants Associated with Micro- and Nanoplastic Particles

Uptake and Release Kinetics of Organic Contaminants Associated with Micro- and Nanoplastic Particles

Drug Administration Routes Impact the Metabolism of a Synthetic Cannabinoid in the Zebrafish Larvae Model

Lipidomics profiling of zebrafish liver through untargeted liquid chromatography‐high resolution mass spectrometry

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